Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2

Carolina Velasco-Aguirre, Francisco Morales-Zavala, Edison Salas-Huenuleo, Eduardo Gallardo-Toledo, Oscar Andonie, Luis Muñoz, Ximena Rojas, Gerardo Acosta, Macarena Sánchez-Navarro, Ernest Giralt, Eyleen Araya, Fernando Albericio, Marcelo Javier Kogan

Resultado de la investigación: Contribución a una revistaArtículo

14 Citas (Scopus)

Resumen

Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.

Idioma originalInglés
Páginas (desde-hasta)2503-2517
Número de páginas15
PublicaciónNanomedicine
Volumen12
N.º20
DOI
EstadoPublicada - 1 oct 2017

Áreas temáticas de ASJC Scopus

  • Bioingeniería
  • Medicina (miscelánea)
  • Ingeniería biomédica
  • Ciencia de los materiales (todo)

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    Velasco-Aguirre, C., Morales-Zavala, F., Salas-Huenuleo, E., Gallardo-Toledo, E., Andonie, O., Muñoz, L., Rojas, X., Acosta, G., Sánchez-Navarro, M., Giralt, E., Araya, E., Albericio, F., & Kogan, M. J. (2017). Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2. Nanomedicine, 12(20), 2503-2517. https://doi.org/10.2217/nnm-2017-0181