Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2

Carolina Velasco-Aguirre, Francisco Morales-Zavala, Edison Salas-Huenuleo, Eduardo Gallardo-Toledo, Oscar Andonie, Luis Muñoz, Ximena Rojas, Gerardo Acosta, Macarena Sánchez-Navarro, Ernest Giralt, Eyleen Araya, Fernando Albericio, Marcelo Javier Kogan

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.

Idioma originalEnglish
Páginas (desde-hasta)2503-2517
Número de páginas15
PublicaciónNanomedicine
Volumen12
N.º20
DOI
EstadoPublished - 1 oct 2017

Huella dactilar

Nanotubes
Neurology
Nanorods
Gold
Central Nervous System
Polyethylene glycols
Brain
Angiopep-2
Endothelial cells
Cytotoxicity
Endocytosis
Blood-Brain Barrier
Nanoparticles
Peptides
Rats
Endothelial Cells

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

Citar esto

Velasco-Aguirre, C., Morales-Zavala, F., Salas-Huenuleo, E., Gallardo-Toledo, E., Andonie, O., Muñoz, L., ... Kogan, M. J. (2017). Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2. Nanomedicine, 12(20), 2503-2517. https://doi.org/10.2217/nnm-2017-0181
Velasco-Aguirre, Carolina ; Morales-Zavala, Francisco ; Salas-Huenuleo, Edison ; Gallardo-Toledo, Eduardo ; Andonie, Oscar ; Muñoz, Luis ; Rojas, Ximena ; Acosta, Gerardo ; Sánchez-Navarro, Macarena ; Giralt, Ernest ; Araya, Eyleen ; Albericio, Fernando ; Kogan, Marcelo Javier. / Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2. En: Nanomedicine. 2017 ; Vol. 12, N.º 20. pp. 2503-2517.
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abstract = "Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.",
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Velasco-Aguirre, C, Morales-Zavala, F, Salas-Huenuleo, E, Gallardo-Toledo, E, Andonie, O, Muñoz, L, Rojas, X, Acosta, G, Sánchez-Navarro, M, Giralt, E, Araya, E, Albericio, F & Kogan, MJ 2017, 'Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2', Nanomedicine, vol. 12, n.º 20, pp. 2503-2517. https://doi.org/10.2217/nnm-2017-0181

Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2. / Velasco-Aguirre, Carolina; Morales-Zavala, Francisco; Salas-Huenuleo, Edison; Gallardo-Toledo, Eduardo; Andonie, Oscar; Muñoz, Luis; Rojas, Ximena; Acosta, Gerardo; Sánchez-Navarro, Macarena; Giralt, Ernest; Araya, Eyleen; Albericio, Fernando; Kogan, Marcelo Javier.

En: Nanomedicine, Vol. 12, N.º 20, 01.10.2017, p. 2503-2517.

Resultado de la investigación: Article

TY - JOUR

T1 - Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2

AU - Velasco-Aguirre, Carolina

AU - Morales-Zavala, Francisco

AU - Salas-Huenuleo, Edison

AU - Gallardo-Toledo, Eduardo

AU - Andonie, Oscar

AU - Muñoz, Luis

AU - Rojas, Ximena

AU - Acosta, Gerardo

AU - Sánchez-Navarro, Macarena

AU - Giralt, Ernest

AU - Araya, Eyleen

AU - Albericio, Fernando

AU - Kogan, Marcelo Javier

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.

AB - Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.

KW - angiopep-2

KW - blood-brain barrier

KW - drug delivery

KW - gold nanoparticle

KW - gold nanorods

KW - photothermal effect

KW - theranostics

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U2 - 10.2217/nnm-2017-0181

DO - 10.2217/nnm-2017-0181

M3 - Article

AN - SCOPUS:85030643955

VL - 12

SP - 2503

EP - 2517

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 20

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Velasco-Aguirre C, Morales-Zavala F, Salas-Huenuleo E, Gallardo-Toledo E, Andonie O, Muñoz L y otros. Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2. Nanomedicine. 2017 oct 1;12(20):2503-2517. https://doi.org/10.2217/nnm-2017-0181