Improved ROS measurement in root hair cells

Silvina Paola Denita Juárez; Silvina Mangano; José M. Estevez

doi:10.1007/978-1-4939-1902-4_6

Improved ROS measurement in root hair cells

Silvina Paola Denita Juárez, Silvina Mangano, José M. Estevez

Facultad de Ciencias de la Vida

Producción científica: Contribución a los tipos de informe/libro › Capítulo › revisión exhaustiva

7 Citas (Scopus)

Resumen

Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA).

Idioma original	Inglés
Título de la publicación alojada	Plant Cell Expansion
Subtítulo de la publicación alojada	Methods and Protocols
Editorial	Springer New York LLC
Páginas	67-71
Número de páginas	5
ISBN (versión digital)	9781493919024
ISBN (versión impresa)	9781493919017
DOI	https://doi.org/10.1007/978-1-4939-1902-4_6
Estado	Publicada - 18 nov. 2014

Áreas temáticas de ASJC Scopus

Ciencias Agrícolas y Biológicas General
Medicina General

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10.1007/978-1-4939-1902-4_6

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abstract = "Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA).",

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AU - Juárez, Silvina Paola Denita

AU - Mangano, Silvina

AU - Estevez, José M.

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Improved ROS measurement in root hair cells

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