Improved ROS measurement in root hair cells

Silvina Paola Denita Juárez, Silvina Mangano, José M. Estevez

Resultado de la investigación: Contribución a los tipos de informe/libroCapítulorevisión exhaustiva

5 Citas (Scopus)

Resumen

Reactive oxygen species (ROS) are recognized as important signaling components in various processes in plants. ROS are produced for NADPH oxidase in different subcellular compartments and they are involved for a wide range of stimuli, such as cell cycle, growth, plant defenses, abiotic stress responses, and abscisic acid signaling in guard cells. In Arabidopsis, root hairs ROS also play a key role in root hair growth and they control the activity of calcium channels required for polar growth (Takeda et al. Science 319:1241-1244, 2008). The production of reactive oxygen species is under a specific molecular control in order to avoid detrimental side effects. Here we describe a protocol to detect ROS by oxidation of a derivative of fluorescein: 2',7-dihidro dicloro fluorescein (H2DCFDA).

Idioma originalInglés
Título de la publicación alojadaPlant Cell Expansion
Subtítulo de la publicación alojadaMethods and Protocols
EditorialSpringer New York LLC
Páginas67-71
Número de páginas5
ISBN (versión digital)9781493919024
ISBN (versión impresa)9781493919017
DOI
EstadoPublicada - 18 nov 2014

Áreas temáticas de ASJC Scopus

  • Agricultura y biología (todo)
  • Medicina (todo)

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