Identification of woolliness response genes in peach fruit after post-harvest treatments

Mauricio González-Agüero, Leonardo Pavez, Freddy Ibáñez, Igor Pacheco, Reinaldo Campos-Vargas, Lee A. Meisel, Ariel Orellana, Julio Retamales, Herman Silva, Mauricio González, Verónica Cambiazo

Resultado de la investigación: Article

64 Citas (Scopus)

Resumen

Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21°C (juicy fruit) were compared with those of fruit stored for 15 d at 4°C and then ripened for 7 d at 21°C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.

Idioma originalEnglish
Páginas (desde-hasta)1973-1986
Número de páginas14
PublicaciónJournal of Experimental Botany
Volumen59
N.º8
DOI
EstadoPublished - 1 may 2008

Huella dactilar

woolliness
postharvest treatment
peaches
Fruit
fruits
Genes
genes
cinnamoyl CoA reductase
Prunus persica
Polygalacturonase
Gene Expression
Elapidae
after-ripening
gene expression
nectarines
shipping
Nylons
Expressed Sequence Tags
polygalacturonase
nylon

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Citar esto

González-Agüero, M., Pavez, L., Ibáñez, F., Pacheco, I., Campos-Vargas, R., Meisel, L. A., ... Cambiazo, V. (2008). Identification of woolliness response genes in peach fruit after post-harvest treatments. Journal of Experimental Botany, 59(8), 1973-1986. https://doi.org/10.1093/jxb/ern069
González-Agüero, Mauricio ; Pavez, Leonardo ; Ibáñez, Freddy ; Pacheco, Igor ; Campos-Vargas, Reinaldo ; Meisel, Lee A. ; Orellana, Ariel ; Retamales, Julio ; Silva, Herman ; González, Mauricio ; Cambiazo, Verónica. / Identification of woolliness response genes in peach fruit after post-harvest treatments. En: Journal of Experimental Botany. 2008 ; Vol. 59, N.º 8. pp. 1973-1986.
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title = "Identification of woolliness response genes in peach fruit after post-harvest treatments",
abstract = "Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21°C (juicy fruit) were compared with those of fruit stored for 15 d at 4°C and then ripened for 7 d at 21°C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90{\%}) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.",
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González-Agüero, M, Pavez, L, Ibáñez, F, Pacheco, I, Campos-Vargas, R, Meisel, LA, Orellana, A, Retamales, J, Silva, H, González, M & Cambiazo, V 2008, 'Identification of woolliness response genes in peach fruit after post-harvest treatments', Journal of Experimental Botany, vol. 59, n.º 8, pp. 1973-1986. https://doi.org/10.1093/jxb/ern069

Identification of woolliness response genes in peach fruit after post-harvest treatments. / González-Agüero, Mauricio; Pavez, Leonardo; Ibáñez, Freddy; Pacheco, Igor; Campos-Vargas, Reinaldo; Meisel, Lee A.; Orellana, Ariel; Retamales, Julio; Silva, Herman; González, Mauricio; Cambiazo, Verónica.

En: Journal of Experimental Botany, Vol. 59, N.º 8, 01.05.2008, p. 1973-1986.

Resultado de la investigación: Article

TY - JOUR

T1 - Identification of woolliness response genes in peach fruit after post-harvest treatments

AU - González-Agüero, Mauricio

AU - Pavez, Leonardo

AU - Ibáñez, Freddy

AU - Pacheco, Igor

AU - Campos-Vargas, Reinaldo

AU - Meisel, Lee A.

AU - Orellana, Ariel

AU - Retamales, Julio

AU - Silva, Herman

AU - González, Mauricio

AU - Cambiazo, Verónica

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21°C (juicy fruit) were compared with those of fruit stored for 15 d at 4°C and then ripened for 7 d at 21°C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.

AB - Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21°C (juicy fruit) were compared with those of fruit stored for 15 d at 4°C and then ripened for 7 d at 21°C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.

KW - Cell wall

KW - Endomembrane traffic

KW - Gene expression

KW - Peach

KW - Woolly fruit

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U2 - 10.1093/jxb/ern069

DO - 10.1093/jxb/ern069

M3 - Article

C2 - 18453640

AN - SCOPUS:44949196773

VL - 59

SP - 1973

EP - 1986

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 8

ER -