Identification of S-alleles associated with self-incompatibility in apricots (Prunus armeniaca L.) using molecular markers

J. M. Donoso, D. Aros, C. Meneses, R. Infante

Resultado de la investigación: Article

6 Citas (Scopus)

Resumen

Gametophytic self-incompatibility, governed by the S-locus, functions in various Prunus species, including the apricot. Knowledge of the S-genotypes in apricot cultivars is essential to establishing productive orchards, defining combinations of compatible cultivars. The identification and cloning of RNases in related species has enabled the use of molecular techniques to characterize S-genotypes in apricot cultivars. To identify the S-alleles associated with self-incompatibility, three pairs of specific primers were tested using PCR, evaluating 31 apricot genotypes. Only the EM-PC2 + EM-PC3 combination produced satisfactory amplifications for the cultivars studied. The results enabled the characterization of 10 apricot cultivars. This PCR-based molecular tool allows S-genotypes to be characterized quickly and efficiently.

Idioma originalEnglish
Páginas (desde-hasta)270-273
Número de páginas4
PublicaciónJournal of Food, Agriculture and Environment
Volumen7
N.º3-4
EstadoPublished - jul 2009

Huella dactilar

self incompatibility
Prunus armeniaca
apricots
cultivar
allele
Alleles
alleles
genotype
genetic markers
Genotype
cultivars
Polymerase Chain Reaction
Prunus
Ribonucleases
orchard
Organism Cloning
molecular cloning
amplification
orchards
marker

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Environmental Science(all)

Citar esto

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Identification of S-alleles associated with self-incompatibility in apricots (Prunus armeniaca L.) using molecular markers. / Donoso, J. M.; Aros, D.; Meneses, C.; Infante, R.

En: Journal of Food, Agriculture and Environment, Vol. 7, N.º 3-4, 07.2009, p. 270-273.

Resultado de la investigación: Article

TY - JOUR

T1 - Identification of S-alleles associated with self-incompatibility in apricots (Prunus armeniaca L.) using molecular markers

AU - Donoso, J. M.

AU - Aros, D.

AU - Meneses, C.

AU - Infante, R.

PY - 2009/7

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AB - Gametophytic self-incompatibility, governed by the S-locus, functions in various Prunus species, including the apricot. Knowledge of the S-genotypes in apricot cultivars is essential to establishing productive orchards, defining combinations of compatible cultivars. The identification and cloning of RNases in related species has enabled the use of molecular techniques to characterize S-genotypes in apricot cultivars. To identify the S-alleles associated with self-incompatibility, three pairs of specific primers were tested using PCR, evaluating 31 apricot genotypes. Only the EM-PC2 + EM-PC3 combination produced satisfactory amplifications for the cultivars studied. The results enabled the characterization of 10 apricot cultivars. This PCR-based molecular tool allows S-genotypes to be characterized quickly and efficiently.

KW - Apricot

KW - Microsatellite

KW - PCR

KW - Prunus armeniaca

KW - Self-incompatibility

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