Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis

Francisca Blanco, Virginia Garretón, Nicolas Frey, Calixto Dominguez, Tomás Pérez-Acle, Dominique Van Der Straeten, Xavier Jordana, Loreto Holuigue

Resultado de la investigación: Article

62 Citas (Scopus)

Resumen

Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.

Idioma originalEnglish
Páginas (desde-hasta)927-944
Número de páginas18
PublicaciónPlant Molecular Biology
Volumen59
N.º6
DOI
EstadoPublished - 1 dic 2005

Huella dactilar

Salicylic Acid
Arabidopsis
salicylic acid
Genes
genes
stress tolerance
Glycosyltransferases
glycosyltransferases
Social Identification
Plant Growth Regulators
Cytoprotection
plant stress
Glutathione Transferase
glutathione transferase
protein kinases
Computer Simulation
Protein Kinases
amplified fragment length polymorphism
Sequence Analysis
signal transduction

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Citar esto

Blanco, F., Garretón, V., Frey, N., Dominguez, C., Pérez-Acle, T., Van Der Straeten, D., ... Holuigue, L. (2005). Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis. Plant Molecular Biology, 59(6), 927-944. https://doi.org/10.1007/s11103-005-2227-x
Blanco, Francisca ; Garretón, Virginia ; Frey, Nicolas ; Dominguez, Calixto ; Pérez-Acle, Tomás ; Van Der Straeten, Dominique ; Jordana, Xavier ; Holuigue, Loreto. / Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis. En: Plant Molecular Biology. 2005 ; Vol. 59, N.º 6. pp. 927-944.
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title = "Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis",
abstract = "Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.",
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Blanco, F, Garretón, V, Frey, N, Dominguez, C, Pérez-Acle, T, Van Der Straeten, D, Jordana, X & Holuigue, L 2005, 'Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis', Plant Molecular Biology, vol. 59, n.º 6, pp. 927-944. https://doi.org/10.1007/s11103-005-2227-x

Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis. / Blanco, Francisca; Garretón, Virginia; Frey, Nicolas; Dominguez, Calixto; Pérez-Acle, Tomás; Van Der Straeten, Dominique; Jordana, Xavier; Holuigue, Loreto.

En: Plant Molecular Biology, Vol. 59, N.º 6, 01.12.2005, p. 927-944.

Resultado de la investigación: Article

TY - JOUR

T1 - Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in arabidopsis

AU - Blanco, Francisca

AU - Garretón, Virginia

AU - Frey, Nicolas

AU - Dominguez, Calixto

AU - Pérez-Acle, Tomás

AU - Van Der Straeten, Dominique

AU - Jordana, Xavier

AU - Holuigue, Loreto

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.

AB - Salicylic acid (SA) plays a crucial role in stress resistance in plants by modifying the expression of a battery of genes. In this paper, we report the identification of a group of early SA-regulated genes of Arabidopsis (activated between 0.5-2.5 h), using the cDNA-amplified fragment length polymorphism technique (cDNA-AFLP). Using 128 different primer combinations, we identified several genes based on their differential expression during SA treatment. Among these, we identified 12 genes up-regulated by SA whose patterns of induction were confirmed by Northern analysis. The identified genes can be grouped into two functional groups: Group 1: genes involved in cell protection (i.e. glycosyltransferases, glutathion S-transferases), and Group 2: genes involved in signal transduction (protein kinases and transcription factors). We also evaluated NPR1 requirement for the induction of the 12 up-regulated genes, and found that only those belonging to Group 2 require this co-activator for their expression. In silico analysis of the promoter sequences of the up-regulated genes, allowed us to identify putative cis-elements over-represented in these genes. Interestingly, as-1-like elements, previously characterized as SA-responsive elements, were specifically over-represented in Group 1 genes. The identification of early SA-regulated genes is an important step towards understanding the complex role of this hormone in plant stress resistance.

KW - As-1

KW - cDNA-AFLP

KW - Cell protection to stress

KW - Early activated genes

KW - NPR1

KW - Salicylic acid

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U2 - 10.1007/s11103-005-2227-x

DO - 10.1007/s11103-005-2227-x

M3 - Article

VL - 59

SP - 927

EP - 944

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 6

ER -