TY - JOUR
T1 - Identification of LOC101927355 as a Novel Biomarker for Preeclampsia
AU - Peñailillo, Reyna
AU - Monteiro, Lara J.
AU - Acuña-Gallardo, Stephanie
AU - García, Felipe
AU - Velásquez, Victoria
AU - Correa, Paula
AU - Díaz, Pilar
AU - Valdebenito, Patricia P.
AU - Navarro, Cristina
AU - Romero, Roberto
AU - Sánchez, Mario
AU - Illanes, Sebastián E.
AU - Nardocci, Gino
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6
Y1 - 2022/6
N2 - Preeclampsia, a disorder with a heterogeneous physiopathology, can be attributed to mater-nal, fetal, and/or placental factors. Long non-coding RNAs (lncRNAs) refer to a class of non-coding RNAs, the essential regulators of biological processes; their differential expression has been associated with the pathogenesis of multiple diseases. The study aimed to identify lncRNAs, expressed in the placentas and plasma of patients who presented with preeclampsia, as potential putative biomarkers of the disease. In silico analysis was performed to determine lncRNAs differentially expressed in the placentas of patients with preeclampsia, using a previously published RNA-Seq dataset. Seven placentas and maternal plasma samples collected at delivery from preterm preeclamptic patients (≤37 gestational weeks of gestation), and controls were used to validate the expression of lncRNAs by qRT-PCR. Six lncRNAs were validated and differentially expressed (p < 0.05) in the preeclampsia and control placentas: UCA1 and HCG4 were found upregulated, and LOC101927355, LINC00551, PART1, and NRAD1 downregulated. Two of these lncRNAs, HCG4 and LOC101927355, were also detected in maternal plasma, the latter showing a significant decrease (p = 0.03) in preeclamptic patients compared to the control group. In silico analyses showed the cytoplasmic location of LOC101927355, which suggests a role in post-transcriptional gene regulation. The detection of LOC101927355 in the placenta and plasma opens new possibilities for understanding the pathogenesis of preeclampsia and for its potential use as a biomarker.
AB - Preeclampsia, a disorder with a heterogeneous physiopathology, can be attributed to mater-nal, fetal, and/or placental factors. Long non-coding RNAs (lncRNAs) refer to a class of non-coding RNAs, the essential regulators of biological processes; their differential expression has been associated with the pathogenesis of multiple diseases. The study aimed to identify lncRNAs, expressed in the placentas and plasma of patients who presented with preeclampsia, as potential putative biomarkers of the disease. In silico analysis was performed to determine lncRNAs differentially expressed in the placentas of patients with preeclampsia, using a previously published RNA-Seq dataset. Seven placentas and maternal plasma samples collected at delivery from preterm preeclamptic patients (≤37 gestational weeks of gestation), and controls were used to validate the expression of lncRNAs by qRT-PCR. Six lncRNAs were validated and differentially expressed (p < 0.05) in the preeclampsia and control placentas: UCA1 and HCG4 were found upregulated, and LOC101927355, LINC00551, PART1, and NRAD1 downregulated. Two of these lncRNAs, HCG4 and LOC101927355, were also detected in maternal plasma, the latter showing a significant decrease (p = 0.03) in preeclamptic patients compared to the control group. In silico analyses showed the cytoplasmic location of LOC101927355, which suggests a role in post-transcriptional gene regulation. The detection of LOC101927355 in the placenta and plasma opens new possibilities for understanding the pathogenesis of preeclampsia and for its potential use as a biomarker.
KW - cellular localization
KW - lncRNAs
KW - placenta
KW - RNA-Seq
UR - http://www.scopus.com/inward/record.url?scp=85131542649&partnerID=8YFLogxK
U2 - 10.3390/biomedicines10061253
DO - 10.3390/biomedicines10061253
M3 - Article
AN - SCOPUS:85131542649
SN - 2227-9059
VL - 10
JO - Biomedicines
JF - Biomedicines
IS - 6
M1 - 1253
ER -