Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing

Gerardo Nuñez-Lillo, Alejandra Cifuentes-Esquivel, Michela Troggio, D. Micheletti, Rodrigo Infante, Reinaldo Campos-Vargas, Ariel Orellana, Francisca Blanco-Herrera, Claudio Meneses

Resultado de la investigación: Article

17 Citas (Scopus)

Resumen

Peach and nectarine quality traits such as flavor, texture, and juiciness are important for consumer acceptance. Maturity date (MD) also plays a role in the fruit-ripening process and is an important factor for marketing fresh fruit. On the other hand, cold storage produces a physiological disorder known as chilling injury where the most important symptom is a lack of juice in the flesh or mealiness (M). In this study, we analyzed an F2 population obtained from a self-pollination of “Venus” nectarine that segregates for MD and M. We built a linkage map with 1,830 SNPs, 7 SSRs and two slow-ripening (SR) morphological markers, spanning 389.2 cM distributed over eight linkage groups (LGs). The SR trait was mapped to LG4 and we compared the whole genome sequences of a SR individual and “Venus” and identified a deletion of 26.6 kb containing ppa008301m (ANAC072) co-localized with the SR trait. Three Quantitative Trait Loci (QTL) for MD were detected; they all co-localize on LG4 between 31.0 and 42.0 cM. Four co-localizing QTLs on LG4 between 33.3 and 40.3 cM were detected for M, explaining 34 % of the phenotypic variation. We identified five and nine candidate genes (CGs) for MD and M from the QTL regions, respectively. Our results suggest that the transcription factors (TFs) ANAC072 and ppa010982m (ERF4) are CGs for both traits. LG4 contains a cluster for genetic factors that possibly regulate M and MD, but functional validation is necessary to unravel the complexity of genetic control responsible for fruit traits.

Idioma originalEnglish
Número de artículo86
PublicaciónTree Genetics and Genomes
Volumen11
N.º4
DOI
EstadoPublished - 3 ago 2015

Huella dactilar

High-Throughput Nucleotide Sequencing
Quantitative Trait Loci
ripening
Prunus persica
Genetic Association Studies
Venus
peaches
quantitative trait loci
Fruit
gene
nectarines
fruit
Pollination
genes
Marketing
Genes
Single Nucleotide Polymorphism
Transcription Factors
fruits
Genome

ASJC Scopus subject areas

  • Forestry
  • Molecular Biology
  • Genetics
  • Horticulture

Citar esto

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title = "Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing",
abstract = "Peach and nectarine quality traits such as flavor, texture, and juiciness are important for consumer acceptance. Maturity date (MD) also plays a role in the fruit-ripening process and is an important factor for marketing fresh fruit. On the other hand, cold storage produces a physiological disorder known as chilling injury where the most important symptom is a lack of juice in the flesh or mealiness (M). In this study, we analyzed an F2 population obtained from a self-pollination of “Venus” nectarine that segregates for MD and M. We built a linkage map with 1,830 SNPs, 7 SSRs and two slow-ripening (SR) morphological markers, spanning 389.2 cM distributed over eight linkage groups (LGs). The SR trait was mapped to LG4 and we compared the whole genome sequences of a SR individual and “Venus” and identified a deletion of 26.6 kb containing ppa008301m (ANAC072) co-localized with the SR trait. Three Quantitative Trait Loci (QTL) for MD were detected; they all co-localize on LG4 between 31.0 and 42.0 cM. Four co-localizing QTLs on LG4 between 33.3 and 40.3 cM were detected for M, explaining 34 {\%} of the phenotypic variation. We identified five and nine candidate genes (CGs) for MD and M from the QTL regions, respectively. Our results suggest that the transcription factors (TFs) ANAC072 and ppa010982m (ERF4) are CGs for both traits. LG4 contains a cluster for genetic factors that possibly regulate M and MD, but functional validation is necessary to unravel the complexity of genetic control responsible for fruit traits.",
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Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing. / Nuñez-Lillo, Gerardo; Cifuentes-Esquivel, Alejandra; Troggio, Michela; Micheletti, D.; Infante, Rodrigo; Campos-Vargas, Reinaldo; Orellana, Ariel; Blanco-Herrera, Francisca; Meneses, Claudio.

En: Tree Genetics and Genomes, Vol. 11, N.º 4, 86, 03.08.2015.

Resultado de la investigación: Article

TY - JOUR

T1 - Identification of candidate genes associated with mealiness and maturity date in peach [Prunus persica (L.) Batsch] using QTL analysis and deep sequencing

AU - Nuñez-Lillo, Gerardo

AU - Cifuentes-Esquivel, Alejandra

AU - Troggio, Michela

AU - Micheletti, D.

AU - Infante, Rodrigo

AU - Campos-Vargas, Reinaldo

AU - Orellana, Ariel

AU - Blanco-Herrera, Francisca

AU - Meneses, Claudio

PY - 2015/8/3

Y1 - 2015/8/3

N2 - Peach and nectarine quality traits such as flavor, texture, and juiciness are important for consumer acceptance. Maturity date (MD) also plays a role in the fruit-ripening process and is an important factor for marketing fresh fruit. On the other hand, cold storage produces a physiological disorder known as chilling injury where the most important symptom is a lack of juice in the flesh or mealiness (M). In this study, we analyzed an F2 population obtained from a self-pollination of “Venus” nectarine that segregates for MD and M. We built a linkage map with 1,830 SNPs, 7 SSRs and two slow-ripening (SR) morphological markers, spanning 389.2 cM distributed over eight linkage groups (LGs). The SR trait was mapped to LG4 and we compared the whole genome sequences of a SR individual and “Venus” and identified a deletion of 26.6 kb containing ppa008301m (ANAC072) co-localized with the SR trait. Three Quantitative Trait Loci (QTL) for MD were detected; they all co-localize on LG4 between 31.0 and 42.0 cM. Four co-localizing QTLs on LG4 between 33.3 and 40.3 cM were detected for M, explaining 34 % of the phenotypic variation. We identified five and nine candidate genes (CGs) for MD and M from the QTL regions, respectively. Our results suggest that the transcription factors (TFs) ANAC072 and ppa010982m (ERF4) are CGs for both traits. LG4 contains a cluster for genetic factors that possibly regulate M and MD, but functional validation is necessary to unravel the complexity of genetic control responsible for fruit traits.

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