Identification of biogenic dimethyl selenodisulfide in the headspace gases above genetically modified Escherichia coli

Jerry W. Swearingen, Danielle P. Frankel, Derie E. Fuentes, Claudia P. Saavedra, Claudio C. Vásquez, Thomas G. Chasteen

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

26 Citas (Scopus)

Resumen

Escherichia coli JM109 cells were modified to express the genes encoded in a 3.8-kb chromosomal DNA fragment from a metalloid-resistant thermophile, Geobacillus stearothermophilus V. Manual headspace extraction was used to collect the gases for gas chromatography with fluorine-induced sulfur chemiluminescence analysis while solid-phase microextraction was used for sample collection in gas chromatography/mass spectrometry (GC/MS) analysis. When grown in the presence of selenate or selenite, these bacteria produced both organo-sulfur and organo-selenium in the headspace gases above the cultures. Organo-sulfur compounds detected were methanethiol, dimethyl sulfide, dimethyl disulfide, and dimethyl trisulfide. Organo-selenium compounds detected were dimethyl selenide and dimethyl diselenide. Two mixed sulfur-selenium compounds, dimethyl selenenyl sulfide and a chromatographically late-eluting compound, were detected. Dimethyl selenodisulfide, CH3SeSSCH3, and dimethyl bis(thio)selenide, CH3SSeSCH3, were synthesized and analyzed by GC/MS and fluorine-induced chemiluminescence to determine which corresponded to the late-eluting compound that was bacterially produced. CH 3SeSSCH3 was positively identified as the compound detected in bacterial headspace above Se-amended cultures. Using GC retention times, the boiling point of CH3SeSSCH3 was estimated to be approximately 192°C. This is the first report of CH3SeSSCH 3 produced by bacterial cultures.

Idioma originalInglés
Páginas (desde-hasta)115-122
Número de páginas8
PublicaciónAnalytical Biochemistry
Volumen348
N.º1
DOI
EstadoPublicada - 1 ene. 2006

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular

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