Identification of a Renibacterium salmoninarum DNA fragment associated with bacterial internalization into CHSE-cultured cells

Nancy P. Maulén, Pedro J. Morales, Dora Aruti, Jaime E. Figueroa, Margarita I. Concha, Manuel Krauskopf, Gloria León

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

We report here the isolation of a Renibacterium salmoninarum DNA sequence capable of transforming a non-invasive Escherichia coli strain into a microorganism able to enter the fish cell line, CHSE-214. Immunofluorescence and electron microscopy techniques were used to assess the acquired invasive phenotype by HB101 E. coli cells, upon transformation with pPMV-189. This plasmid carries a 2282-bp R. salmoninarum DNA segment. The invasive phenotype is conserved upon deletion of approximately 1000 bp at the 3' end of the insert. The remaining segment contains an ORF region encoding a putative protein of about 30 kDa.

Idioma originalInglés
Páginas (desde-hasta)37-43
Número de páginas7
PublicaciónFEMS Microbiology Letters
Volumen135
N.º1
DOI
EstadoPublicada - 1 ene 1996

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular
  • Genética

Huella

Profundice en los temas de investigación de 'Identification of a Renibacterium salmoninarum DNA fragment associated with bacterial internalization into CHSE-cultured cells'. En conjunto forman una huella única.

Citar esto