Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD

Svenning Rune Møller, Xueying Yi, Silvia Melina Velásquez, Sascha Gille, Pernille Louise Munke Hansen, Christian P. Poulsen, Carl Erik Olsen, Martin Rejzek, Harriet Parsons, Yang Zhang, Hans H. Wandall, Henrik Clausen, Robert A. Field, Markus Pauly, Jose M. Estevez, Jesper Harholt, Peter Ulvskov, Bent Larsen Petersen

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae.

Idioma originalEnglish
Número de artículo45341
PublicaciónScientific Reports
Volumen7
DOI
EstadoPublished - 30 mar 2017

Huella dactilar

Plant Cells
Transferases
Cell Wall
Glycoproteins
Viridiplantae
Phenotype
Glycosyltransferases
Chlorophyta
Arabinose
Hydroxyproline
Galactose
Glycosylation
Arabidopsis
Serine
Polysaccharides
Tyrosine
Polymers
Carbohydrates
Enzymes
Genes

ASJC Scopus subject areas

  • General

Citar esto

Møller, S. R., Yi, X., Velásquez, S. M., Gille, S., Hansen, P. L. M., Poulsen, C. P., ... Petersen, B. L. (2017). Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD. Scientific Reports, 7, [45341]. https://doi.org/10.1038/srep45341
Møller, Svenning Rune ; Yi, Xueying ; Velásquez, Silvia Melina ; Gille, Sascha ; Hansen, Pernille Louise Munke ; Poulsen, Christian P. ; Olsen, Carl Erik ; Rejzek, Martin ; Parsons, Harriet ; Zhang, Yang ; Wandall, Hans H. ; Clausen, Henrik ; Field, Robert A. ; Pauly, Markus ; Estevez, Jose M. ; Harholt, Jesper ; Ulvskov, Peter ; Petersen, Bent Larsen. / Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD. En: Scientific Reports. 2017 ; Vol. 7.
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abstract = "Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae.",
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Møller, SR, Yi, X, Velásquez, SM, Gille, S, Hansen, PLM, Poulsen, CP, Olsen, CE, Rejzek, M, Parsons, H, Zhang, Y, Wandall, HH, Clausen, H, Field, RA, Pauly, M, Estevez, JM, Harholt, J, Ulvskov, P & Petersen, BL 2017, 'Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD', Scientific Reports, vol. 7, 45341. https://doi.org/10.1038/srep45341

Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD. / Møller, Svenning Rune; Yi, Xueying; Velásquez, Silvia Melina; Gille, Sascha; Hansen, Pernille Louise Munke; Poulsen, Christian P.; Olsen, Carl Erik; Rejzek, Martin; Parsons, Harriet; Zhang, Yang; Wandall, Hans H.; Clausen, Henrik; Field, Robert A.; Pauly, Markus; Estevez, Jose M.; Harholt, Jesper; Ulvskov, Peter; Petersen, Bent Larsen.

En: Scientific Reports, Vol. 7, 45341, 30.03.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD

AU - Møller, Svenning Rune

AU - Yi, Xueying

AU - Velásquez, Silvia Melina

AU - Gille, Sascha

AU - Hansen, Pernille Louise Munke

AU - Poulsen, Christian P.

AU - Olsen, Carl Erik

AU - Rejzek, Martin

AU - Parsons, Harriet

AU - Zhang, Yang

AU - Wandall, Hans H.

AU - Clausen, Henrik

AU - Field, Robert A.

AU - Pauly, Markus

AU - Estevez, Jose M.

AU - Harholt, Jesper

AU - Ulvskov, Peter

AU - Petersen, Bent Larsen

PY - 2017/3/30

Y1 - 2017/3/30

N2 - Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae.

AB - Extensins are plant cell wall glycoproteins that act as scaffolds for the deposition of the main wall carbohydrate polymers, which are interlocked into the supramolecular wall structure through intra- and inter-molecular iso-di-tyrosine crosslinks within the extensin backbone. In the conserved canonical extensin repeat, Ser-Hyp 4, serine and the consecutive C4-hydroxyprolines (Hyps) are substituted with an α-galactose and 1-5 β- or α-linked arabinofuranoses (Arafs), respectively. These modifications are required for correct extended structure and function of the extensin network. Here, we identified a single Arabidopsis thaliana gene, At3g57630, in clade E of the inverting Glycosyltransferase family GT47 as a candidate for the transfer of Araf to Hyp-arabinofuranotriose (Hyp-β1,4Araf-β1,2Araf-β1,2Araf) side chains in an α-linkage, to yield Hyp-Araf 4 which is exclusively found in extensins. T-DNA knock-out mutants of At3g57630 showed a truncated root hair phenotype, as seen for mutants of all hitherto characterized extensin glycosylation enzymes; both root hair and glycan phenotypes were restored upon reintroduction of At3g57630. At3g57630 was named Extensin Arabinose Deficient transferase, ExAD, accordingly. The occurrence of ExAD orthologs within the Viridiplantae along with its' product, Hyp-Araf 4, point to ExAD being an evolutionary hallmark of terrestrial plants and charophyte green algae.

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Møller SR, Yi X, Velásquez SM, Gille S, Hansen PLM, Poulsen CP y otros. Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD. Scientific Reports. 2017 mar 30;7. 45341. https://doi.org/10.1038/srep45341