Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in Nicotiana tabacum plants

Claudia Stange, J. T. Matus, Alvaro Elorza, Patricio Arce-Johnson

Resultado de la investigación: Article

21 Citas (Scopus)

Resumen

Nicotiana tabacum cv. Xanthi nn plants are susceptible to infection by most tobamoviruses (TMV). However, such plants display a partial hypersensitive resistance response (HR-like response) to TMV-Cg. The genetic mechanism of the HR-like response has yet not been determined, but it may involve a gene with a function similar to that of a resistance gene, responsible for HR in resistant plants. We have cloned a gene homologous to the resistance N gene, named NH, from Nicotiana Xanthi nn plants. The coding region of NH is 5.028 base pairs (bp) long and has 82.6% nucleotide identity with the N gene. In contrast to the N gene, the NH gene lacks intron 4 and does not have sites for alternative splicing of intron 3. Analysis of its sequence revealed that NH belongs to the TIR/NSB/LRR gene class. We were able to detect stable levels of NH-transcript in Nicotiana Xanthi nn plants from 0 to 18 h post-inoculation (hpi) with TMV-Cg. Transcript levels increased slightly at 24 hpi and dropped below basal values at 48 hpi. The NH transcript was also detected in a range of resistant Nicotiana plants (N. tabacum Xanthi NN, N. glutinosa, N. glauca and N. rustica) suggesting that NH is a homologue of the N gene, rather than an allele. We have cloned and characterised the NH gene (GenBank ace. no. bankit598573 AY535010) from nn susceptible plants and postulate that this gene might be involved in the HR-like response seen in these plants.

Idioma originalEnglish
Páginas (desde-hasta)149-158
Número de páginas10
PublicaciónFunctional Plant Biology
Volumen31
N.º2
DOI
EstadoPublished - 2004

Huella dactilar

Tobacco mosaic virus
Nicotiana tabacum
genes
Tobamovirus
Nicotiana
introns
Nicotiana glauca
Nicotiana rustica
Nicotiana glutinosa
biological resistance
alternative splicing
sequence analysis
nucleotides
alleles

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Citar esto

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title = "Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in Nicotiana tabacum plants",
abstract = "Nicotiana tabacum cv. Xanthi nn plants are susceptible to infection by most tobamoviruses (TMV). However, such plants display a partial hypersensitive resistance response (HR-like response) to TMV-Cg. The genetic mechanism of the HR-like response has yet not been determined, but it may involve a gene with a function similar to that of a resistance gene, responsible for HR in resistant plants. We have cloned a gene homologous to the resistance N gene, named NH, from Nicotiana Xanthi nn plants. The coding region of NH is 5.028 base pairs (bp) long and has 82.6{\%} nucleotide identity with the N gene. In contrast to the N gene, the NH gene lacks intron 4 and does not have sites for alternative splicing of intron 3. Analysis of its sequence revealed that NH belongs to the TIR/NSB/LRR gene class. We were able to detect stable levels of NH-transcript in Nicotiana Xanthi nn plants from 0 to 18 h post-inoculation (hpi) with TMV-Cg. Transcript levels increased slightly at 24 hpi and dropped below basal values at 48 hpi. The NH transcript was also detected in a range of resistant Nicotiana plants (N. tabacum Xanthi NN, N. glutinosa, N. glauca and N. rustica) suggesting that NH is a homologue of the N gene, rather than an allele. We have cloned and characterised the NH gene (GenBank ace. no. bankit598573 AY535010) from nn susceptible plants and postulate that this gene might be involved in the HR-like response seen in these plants.",
keywords = "Disease resistance, HR-like, Hypersensitive response, N gene, TIR/NBS/LRR receptor, TMV-Cg",
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Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in Nicotiana tabacum plants. / Stange, Claudia; Matus, J. T.; Elorza, Alvaro; Arce-Johnson, Patricio.

En: Functional Plant Biology, Vol. 31, N.º 2, 2004, p. 149-158.

Resultado de la investigación: Article

TY - JOUR

T1 - Identification and characterization of a novel tobacco mosaic virus resistance N gene homologue in Nicotiana tabacum plants

AU - Stange, Claudia

AU - Matus, J. T.

AU - Elorza, Alvaro

AU - Arce-Johnson, Patricio

PY - 2004

Y1 - 2004

N2 - Nicotiana tabacum cv. Xanthi nn plants are susceptible to infection by most tobamoviruses (TMV). However, such plants display a partial hypersensitive resistance response (HR-like response) to TMV-Cg. The genetic mechanism of the HR-like response has yet not been determined, but it may involve a gene with a function similar to that of a resistance gene, responsible for HR in resistant plants. We have cloned a gene homologous to the resistance N gene, named NH, from Nicotiana Xanthi nn plants. The coding region of NH is 5.028 base pairs (bp) long and has 82.6% nucleotide identity with the N gene. In contrast to the N gene, the NH gene lacks intron 4 and does not have sites for alternative splicing of intron 3. Analysis of its sequence revealed that NH belongs to the TIR/NSB/LRR gene class. We were able to detect stable levels of NH-transcript in Nicotiana Xanthi nn plants from 0 to 18 h post-inoculation (hpi) with TMV-Cg. Transcript levels increased slightly at 24 hpi and dropped below basal values at 48 hpi. The NH transcript was also detected in a range of resistant Nicotiana plants (N. tabacum Xanthi NN, N. glutinosa, N. glauca and N. rustica) suggesting that NH is a homologue of the N gene, rather than an allele. We have cloned and characterised the NH gene (GenBank ace. no. bankit598573 AY535010) from nn susceptible plants and postulate that this gene might be involved in the HR-like response seen in these plants.

AB - Nicotiana tabacum cv. Xanthi nn plants are susceptible to infection by most tobamoviruses (TMV). However, such plants display a partial hypersensitive resistance response (HR-like response) to TMV-Cg. The genetic mechanism of the HR-like response has yet not been determined, but it may involve a gene with a function similar to that of a resistance gene, responsible for HR in resistant plants. We have cloned a gene homologous to the resistance N gene, named NH, from Nicotiana Xanthi nn plants. The coding region of NH is 5.028 base pairs (bp) long and has 82.6% nucleotide identity with the N gene. In contrast to the N gene, the NH gene lacks intron 4 and does not have sites for alternative splicing of intron 3. Analysis of its sequence revealed that NH belongs to the TIR/NSB/LRR gene class. We were able to detect stable levels of NH-transcript in Nicotiana Xanthi nn plants from 0 to 18 h post-inoculation (hpi) with TMV-Cg. Transcript levels increased slightly at 24 hpi and dropped below basal values at 48 hpi. The NH transcript was also detected in a range of resistant Nicotiana plants (N. tabacum Xanthi NN, N. glutinosa, N. glauca and N. rustica) suggesting that NH is a homologue of the N gene, rather than an allele. We have cloned and characterised the NH gene (GenBank ace. no. bankit598573 AY535010) from nn susceptible plants and postulate that this gene might be involved in the HR-like response seen in these plants.

KW - Disease resistance

KW - HR-like

KW - Hypersensitive response

KW - N gene

KW - TIR/NBS/LRR receptor

KW - TMV-Cg

UR - http://www.scopus.com/inward/record.url?scp=1842425002&partnerID=8YFLogxK

U2 - 10.1071/FP03160

DO - 10.1071/FP03160

M3 - Article

AN - SCOPUS:1842425002

VL - 31

SP - 149

EP - 158

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 2

ER -