Human preimplantation development in vivo: ultrastructural observations.

J. Pereda, H. B. Croxatto

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

The ultrastructure of a 2-cell, 4-cell, 7-cell and 16-cell human embryos fertilised and developed in vivo were compared using transmission electron microscopy. Among the structures which exhibited more notable changes throughout these developmental stages, were the mitochondria, ribosomes, nuclear envelope and nucleolus. Mitochondria which were initially round, dense with cristae oriented parallel to the surface changed to oval with less dense mitochondrial matrix and with cristae perpendicular to the mitochondrial membrane. Ribosomes and polyribosomes decreased in amount from 2 to 7-cell stages and increased again at the 16-cell stage. The nuclear envelope exhibited intense blebbing activity at the 4-cell stage, less at 7 and none at 16. The nucleolus initially very dense was progressively infiltrated by chromatin, became reticular at the 7-cell stage and appeared fully mature at the 16-cell stage. This study discloses structural details of human preimplantation embryos which may be of interest in the assessment of comparable material obtained in vitro. Some unique features that may have bearing on the ontogenesis of cell organelles and cell differentiation are discussed.

Idioma originalEnglish
Páginas (desde-hasta)480-488
Número de páginas9
PublicaciónAnnals of the Academy of Medicine Singapore
Volumen21
N.º4
EstadoPublished - jul 1992

Huella dactilar

Human Development
Nuclear Envelope
Ribosomes
Mitochondria
Polyribosomes
Mitochondrial Membranes
Blastocyst
Blister
Transmission Electron Microscopy
Organelles
Chromatin
Cell Differentiation
Embryonic Structures

ASJC Scopus subject areas

  • Medicine(all)

Citar esto

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Human preimplantation development in vivo : ultrastructural observations. / Pereda, J.; Croxatto, H. B.

En: Annals of the Academy of Medicine Singapore, Vol. 21, N.º 4, 07.1992, p. 480-488.

Resultado de la investigación: Article

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T1 - Human preimplantation development in vivo

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AU - Croxatto, H. B.

PY - 1992/7

Y1 - 1992/7

N2 - The ultrastructure of a 2-cell, 4-cell, 7-cell and 16-cell human embryos fertilised and developed in vivo were compared using transmission electron microscopy. Among the structures which exhibited more notable changes throughout these developmental stages, were the mitochondria, ribosomes, nuclear envelope and nucleolus. Mitochondria which were initially round, dense with cristae oriented parallel to the surface changed to oval with less dense mitochondrial matrix and with cristae perpendicular to the mitochondrial membrane. Ribosomes and polyribosomes decreased in amount from 2 to 7-cell stages and increased again at the 16-cell stage. The nuclear envelope exhibited intense blebbing activity at the 4-cell stage, less at 7 and none at 16. The nucleolus initially very dense was progressively infiltrated by chromatin, became reticular at the 7-cell stage and appeared fully mature at the 16-cell stage. This study discloses structural details of human preimplantation embryos which may be of interest in the assessment of comparable material obtained in vitro. Some unique features that may have bearing on the ontogenesis of cell organelles and cell differentiation are discussed.

AB - The ultrastructure of a 2-cell, 4-cell, 7-cell and 16-cell human embryos fertilised and developed in vivo were compared using transmission electron microscopy. Among the structures which exhibited more notable changes throughout these developmental stages, were the mitochondria, ribosomes, nuclear envelope and nucleolus. Mitochondria which were initially round, dense with cristae oriented parallel to the surface changed to oval with less dense mitochondrial matrix and with cristae perpendicular to the mitochondrial membrane. Ribosomes and polyribosomes decreased in amount from 2 to 7-cell stages and increased again at the 16-cell stage. The nuclear envelope exhibited intense blebbing activity at the 4-cell stage, less at 7 and none at 16. The nucleolus initially very dense was progressively infiltrated by chromatin, became reticular at the 7-cell stage and appeared fully mature at the 16-cell stage. This study discloses structural details of human preimplantation embryos which may be of interest in the assessment of comparable material obtained in vitro. Some unique features that may have bearing on the ontogenesis of cell organelles and cell differentiation are discussed.

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