Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol

Carla Klattenhoff, Martín Montecino, Ximena Soto, Leonardo Guzmán, Ximena Romo, María De Los Angeles García, Britt Mellstrom, José Ramón Naranjo, María Victoria Hinrichs, Juan Olate

Resultado de la investigación: Article

45 Citas (Scopus)

Resumen

Heterotrimeric G-proteins transduce signals from heptahelical transmembrane receptors to different effector systems, regulating diverse complex intracellular pathways and functions. In brain, facilitation of depolarization-induced neurotransmitter release for synaptic transmission is mediated by Gsα and Gqα. To identify effectors for Gα-proteins, we performed a yeast two-hybrid screening of a human brain cDNA library, using the human Gαs protein as a bait. We identified a protein member of the synembryn family as one of the interacting proteins. Extending the study to other Gα subunits, we found that Gqα also interacts with synembryn, and these interactions were confirmed by in vitro pull down studies and by in vivo confocal laser microscopy analysis. Furthermore, synembryn was shown to translocate to the plasma membrane in response to carbachol and isoproterenol. This study supports recent findings in C. elegans where, through genetic studies, synembryn was shown to act together with Gqα regulating neuronal transmitter release. Based on these observations, we propose that synembryn is playing a similar role in human neuronal cells.

Idioma originalEnglish
Páginas (desde-hasta)151-157
Número de páginas7
PublicaciónJournal of Cellular Physiology
Volumen195
N.º2
DOI
EstadoPublished - 1 may 2003

Huella dactilar

Carbachol
Cell membranes
Isoproterenol
Brain
Cell Membrane
Confocal Microscopy
Heterotrimeric GTP-Binding Proteins
Proteins
Depolarization
Gene Library
GTP-Binding Proteins
Synaptic Transmission
Yeast
Neurotransmitter Agents
Transmitters
Microscopic examination
Screening
Yeasts
Lasers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Citar esto

Klattenhoff, Carla ; Montecino, Martín ; Soto, Ximena ; Guzmán, Leonardo ; Romo, Ximena ; De Los Angeles García, María ; Mellstrom, Britt ; Naranjo, José Ramón ; Hinrichs, María Victoria ; Olate, Juan. / Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol. En: Journal of Cellular Physiology. 2003 ; Vol. 195, N.º 2. pp. 151-157.
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title = "Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol",
abstract = "Heterotrimeric G-proteins transduce signals from heptahelical transmembrane receptors to different effector systems, regulating diverse complex intracellular pathways and functions. In brain, facilitation of depolarization-induced neurotransmitter release for synaptic transmission is mediated by Gsα and Gqα. To identify effectors for Gα-proteins, we performed a yeast two-hybrid screening of a human brain cDNA library, using the human Gαs protein as a bait. We identified a protein member of the synembryn family as one of the interacting proteins. Extending the study to other Gα subunits, we found that Gqα also interacts with synembryn, and these interactions were confirmed by in vitro pull down studies and by in vivo confocal laser microscopy analysis. Furthermore, synembryn was shown to translocate to the plasma membrane in response to carbachol and isoproterenol. This study supports recent findings in C. elegans where, through genetic studies, synembryn was shown to act together with Gqα regulating neuronal transmitter release. Based on these observations, we propose that synembryn is playing a similar role in human neuronal cells.",
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Klattenhoff, C, Montecino, M, Soto, X, Guzmán, L, Romo, X, De Los Angeles García, M, Mellstrom, B, Naranjo, JR, Hinrichs, MV & Olate, J 2003, 'Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol', Journal of Cellular Physiology, vol. 195, n.º 2, pp. 151-157. https://doi.org/10.1002/jcp.10300

Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol. / Klattenhoff, Carla; Montecino, Martín; Soto, Ximena; Guzmán, Leonardo; Romo, Ximena; De Los Angeles García, María; Mellstrom, Britt; Naranjo, José Ramón; Hinrichs, María Victoria; Olate, Juan.

En: Journal of Cellular Physiology, Vol. 195, N.º 2, 01.05.2003, p. 151-157.

Resultado de la investigación: Article

TY - JOUR

T1 - Human brain synembryn interacts with Gsα and Gqα and is translocated to the plasma membrane in response to isoproterenol and carbachol

AU - Klattenhoff, Carla

AU - Montecino, Martín

AU - Soto, Ximena

AU - Guzmán, Leonardo

AU - Romo, Ximena

AU - De Los Angeles García, María

AU - Mellstrom, Britt

AU - Naranjo, José Ramón

AU - Hinrichs, María Victoria

AU - Olate, Juan

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Heterotrimeric G-proteins transduce signals from heptahelical transmembrane receptors to different effector systems, regulating diverse complex intracellular pathways and functions. In brain, facilitation of depolarization-induced neurotransmitter release for synaptic transmission is mediated by Gsα and Gqα. To identify effectors for Gα-proteins, we performed a yeast two-hybrid screening of a human brain cDNA library, using the human Gαs protein as a bait. We identified a protein member of the synembryn family as one of the interacting proteins. Extending the study to other Gα subunits, we found that Gqα also interacts with synembryn, and these interactions were confirmed by in vitro pull down studies and by in vivo confocal laser microscopy analysis. Furthermore, synembryn was shown to translocate to the plasma membrane in response to carbachol and isoproterenol. This study supports recent findings in C. elegans where, through genetic studies, synembryn was shown to act together with Gqα regulating neuronal transmitter release. Based on these observations, we propose that synembryn is playing a similar role in human neuronal cells.

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