HIV-1 mRNA 3′ End Processing Is Distinctively Regulated by eIF3f, CDK11, and Splice Factor 9G8

Susana T. Valente, Greg M. Gilmartin, Krishnan Venkatarama, Gloria Arriagada, Stephen P. Goff

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

40 Citas (Scopus)

Resumen

A genetic screen previously identified the N-terminal 91 amino acids of the eukaryotic initiation factor 3 subunit f (N91-eIF3f) as a potent inhibitor of HIV-1 replication. Overexpression of N91-eIF3f or full-length eIF3f reduced the level of HIV-1 mRNAs in the infected cell. Here we show that N91-eIF3f and eIF3f act by specifically blocking the 3′ end processing of the HIV-1 pre-mRNA both in vivo and in vitro. Furthermore, the results suggest that eIF3f mediates this restriction of HIV-1 expression through the previously unsuspected involvement of a set of factors that includes eIF3f, the SR protein 9G8, and the cyclin-dependent kinase 11 (CDK11). eIF3f affects HIV-1 3′ end processing by modulating the sequence-specific recognition of the HIV-1 pre-mRNA by 9G8.

Idioma originalInglés
Páginas (desde-hasta)279-289
Número de páginas11
PublicaciónMolecular Cell
Volumen36
N.º2
DOI
EstadoPublicada - 23 oct 2009

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Biología celular

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