Histamine reduces gap junctional communication of human tonsil high endothelial cells in culture

Xavier F. Figueroa, Karina Alviña, Agustín D. Martínez, Gladys Garcés, Mario Rosemblatt, Mauricio P. Boric, Juan C. Sáez

Resultado de la investigación: Contribución a una revistaArtículo

12 Citas (Scopus)


The regulation of gap junctional communication by histamine was studied in primary cultures of human tonsil high endothelial cells (HUTECs). We evaluated intercellular communication, levels, state of phosphorylation, and cellular distribution of gap junction protein subunits, mainly connexin (Cx)43. Histamine induced a time-dependent reduction in dye coupling (Lucifer yellow) associated with reduction in connexin43 localized at cell-cell appositions (immunofluorescence), without changes in levels and phosphorylation state of connexin43 (immunoblots). These effects were prevented with chlorpheniramine, an H1 receptor blocker; indomethacin, a cyclooxygenase blocker; or GF109203X, a protein kinase C inhibitor. Treatment with phorbol myristate acetate, a protein kinase C activator, and 4bromo (4Br)-A23187, a calcium ionophore, mimicked the histamine-induced effects on dye coupling. 8Bromo-cAMP doubled the dye coupling extent and prevented the histamine-induced reduction in incidence of dye coupling. After 24-h histamine treatment, known to desensitize H1 receptors, reapplication of histamine increased cell coupling in a way prevented by ranitidine, an H2 receptor blocker. Thus, activation of H1 and H2 receptors, which increase intracellular levels of free Ca2+ and cAMP, respectively, may affect gap junctional communication in opposite ways. Stabilization of actin filaments with phalloidine diminished but did not totally prevent histamine-induced cell shape changes and reduction in dye coupling. Hence, the histamine-induced reduction in gap junctional communication between HUTEC is mediated by cytoskeleton-dependent and -independent mechanisms and might contribute to modulate endothelial function in lymphoid tissue.

Idioma originalInglés
Páginas (desde-hasta)247-257
Número de páginas11
PublicaciónMicrovascular Research
EstadoPublicada - 1 ene 2004

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Cardiología y medicina cardiovascular
  • Biología celular

Huella Profundice en los temas de investigación de 'Histamine reduces gap junctional communication of human tonsil high endothelial cells in culture'. En conjunto forman una huella única.

  • Citar esto

    Figueroa, X. F., Alviña, K., Martínez, A. D., Garcés, G., Rosemblatt, M., Boric, M. P., & Sáez, J. C. (2004). Histamine reduces gap junctional communication of human tonsil high endothelial cells in culture. Microvascular Research, 68(3), 247-257. https://doi.org/10.1016/j.mvr.2004.06.009