Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA1 Val on BD-cellulose

Eduardo Rosenmann, Manuel Krauskopf

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

The chromatographic behaviour of E.coli tRNA1Val, Val-tRNA1Val and Phe-tRNA1Val was studied on BD-cellulose columns. At pH 4.0 and 4°C the elution position of Phe-tRNA1Val was not affected by the presence or absence of Mg2+, whereas Val-tRNA1Val was slightly retarded when Mg2+ was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNA1Val eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.

Idioma originalEnglish
Páginas (desde-hasta)935-944
Número de páginas10
PublicaciónNucleic Acids Research
Volumen1
N.º8
DOI
EstadoPublished - 1 ago 1974

Huella dactilar

Cellulose
Fractionation
Transfer RNA
Purification
Escherichia coli
Escherichia Coli
Amino Acids
Amino acids
Influence
benzoylated DEAE-cellulose

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Citar esto

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Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E.coli phenylalanyl-tRNA1 Val on BD-cellulose. / Rosenmann, Eduardo; Krauskopf, Manuel.

En: Nucleic Acids Research, Vol. 1, N.º 8, 01.08.1974, p. 935-944.

Resultado de la investigación: Article

TY - JOUR

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AU - Rosenmann, Eduardo

AU - Krauskopf, Manuel

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