Heterologous expression of Escherichia coli porin genes in Salmonella typhi Ty2: regulation by medium osmolarity, temperature and oxygen availability

Inés Contreras, Lorena Munoz, Cecilia S. Toro, Guido C. Mora

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

Electrophoretic analysis of outer membrane proteins showed that Salmonella typhi OmpC expression is not reciprocally regulated relative to OmpF as described for Escherichia coli and S. typhimurium. When bacteria were grown in minimal media, both OmpC and OmpF were repressed as the osmolarity increased. However, in Luria broth, expression of OmpC was slightly induced by osmolarity up to 0.3 osmM. Plasmids bearing E. coli ompC-lacZ or ompF-lacZ gene fusions were studied for their expression in S. typhi and E. coli. Under anaerobic growth conditions, expression of ompC-lacZ in S. typhi was maximal at 0.16 osmM, while in E. coli expression was maximal at 0.7 osmM. ompF-lacZ expression was similarly repressed by medium osmolarity and anaerobiosis in both species. In contrast, a drastic difference in the regulation of OmpF by temperature was observed; at 37 °C ompF-lacZ expression was repressed in E. coli. while in S. typhi it was induced.

Idioma originalInglés
Páginas (desde-hasta)105-111
Número de páginas7
PublicaciónFEMS Microbiology Letters
Volumen133
N.º1-2
DOI
EstadoPublicada - 1 nov 1995

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular
  • Genética

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