Heparan sulfate proteoglycans exert positive and negative effects in Shh activity

Héctor Carrasco, Gonzalo H. Olivares, Fernando Faunes, Carlos Oliva, Juan Larraín

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

24 Citas (Scopus)


Hedgehog (Hh) proteins are morphogens involved in short-and long-range effects during early embryonic development. Genetic analysis in fly and vertebrate embryos showed that heparan sulfate proteoglycans (HSPGs) are required for Hh transport and signaling. To further understand how HSPGs regulate Sonic hedgehog (Shh), we performed experiments using cell culture and biochemical assays. When the synthesis of HSPGs was reduced, a decrease in Shh activity was observed. Contrary to that, addition of a peptide that competes the binding of Shh to HSPGs resulted in augmentation of Shh activity. From these results, we concluded that HSPGs exert positive and negative effects in Shh activity. This dual effect correlates with the finding that Shh interacts preferentially with two HSPGs. The current model for the role of HSPGs in Shh diffusion is discussed in view of our findings.

Idioma originalInglés
Páginas (desde-hasta)831-838
Número de páginas8
PublicaciónJournal of Cellular Biochemistry
EstadoPublicada - 1 nov. 2005

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología celular


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