Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection

Janyra A. Espinoza, Miguel A. León, Pablo F. Céspedes, Roberto S. Gómez, Gisela Canedo-Marroquín, Sebastían A. Riquelme, Francisco J. Salazar-Echegarai, Phillipe Blancou, Thomas Simon, Ignacio Anegon, Margarita K. Lay, Pablo A. González, Claudia A. Riedel, Susan M. Bueno, Alexis M. Kalergis

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

Human respiratory syncytial virus (hRSV) is the leading cause of severe lower respiratory tract infections in children. The development of novel prophylactic and therapeutic antiviral drugs against hRSV is imperative to control the burden of disease in the susceptible population. In this study, we examined the effects of inducing the activity of the host enzyme heme oxygenase-1 (HO-1) on hRSV replication and pathogenesis on lung inflammation induced by this virus. Our results show that after hRSV infection, HO-1 induction with metalloporphyrin cobalt protoporphyrin IX significantly reduces the loss of body weight due to hRSV-induced disease. Further, HO-1 induction also decreased viral replication and lung inflammation, as evidenced by a reduced neutrophil infiltration into the airways, with diminished cytokine and chemokine production and reduced T cell function. Concomitantly, upon cobalt protoporphyrin IX treatment, there is a significant upregulation in the production of IFN-α/β mRNAs in the lungs. Furthermore, similar antiviral and protective effects occur by inducing the expression of human HO-1 in MHC class II+ cells in transgenic mice. Finally, in vitro data suggest that HO-1 induction can modulate the susceptibility of cells, especially the airway epithelial cells, to hRSV infection.

Idioma originalEnglish
Páginas (desde-hasta)212-223
Número de páginas12
PublicaciónJournal of Immunology
Volumen199
N.º1
DOI
EstadoPublished - 1 jul 2017

Huella dactilar

Human respiratory syncytial virus
Heme Oxygenase-1
Virus Replication
Lung
Infection
Respiratory Syncytial Virus Infections
Antiviral Agents
Pneumonia
Metalloporphyrins
Neutrophil Infiltration
Virus Diseases
Chemokines
Respiratory Tract Infections
Transgenic Mice
Up-Regulation
Epithelial Cells
Body Weight
Cytokines
Viruses
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Citar esto

Espinoza, J. A., León, M. A., Céspedes, P. F., Gómez, R. S., Canedo-Marroquín, G., Riquelme, S. A., ... Kalergis, A. M. (2017). Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection. Journal of Immunology, 199(1), 212-223. https://doi.org/10.4049/jimmunol.1601414
Espinoza, Janyra A. ; León, Miguel A. ; Céspedes, Pablo F. ; Gómez, Roberto S. ; Canedo-Marroquín, Gisela ; Riquelme, Sebastían A. ; Salazar-Echegarai, Francisco J. ; Blancou, Phillipe ; Simon, Thomas ; Anegon, Ignacio ; Lay, Margarita K. ; González, Pablo A. ; Riedel, Claudia A. ; Bueno, Susan M. ; Kalergis, Alexis M. / Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection. En: Journal of Immunology. 2017 ; Vol. 199, N.º 1. pp. 212-223.
@article{7797cea05de5433a8085672ebd589ea9,
title = "Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection",
abstract = "Human respiratory syncytial virus (hRSV) is the leading cause of severe lower respiratory tract infections in children. The development of novel prophylactic and therapeutic antiviral drugs against hRSV is imperative to control the burden of disease in the susceptible population. In this study, we examined the effects of inducing the activity of the host enzyme heme oxygenase-1 (HO-1) on hRSV replication and pathogenesis on lung inflammation induced by this virus. Our results show that after hRSV infection, HO-1 induction with metalloporphyrin cobalt protoporphyrin IX significantly reduces the loss of body weight due to hRSV-induced disease. Further, HO-1 induction also decreased viral replication and lung inflammation, as evidenced by a reduced neutrophil infiltration into the airways, with diminished cytokine and chemokine production and reduced T cell function. Concomitantly, upon cobalt protoporphyrin IX treatment, there is a significant upregulation in the production of IFN-α/β mRNAs in the lungs. Furthermore, similar antiviral and protective effects occur by inducing the expression of human HO-1 in MHC class II+ cells in transgenic mice. Finally, in vitro data suggest that HO-1 induction can modulate the susceptibility of cells, especially the airway epithelial cells, to hRSV infection.",
author = "Espinoza, {Janyra A.} and Le{\'o}n, {Miguel A.} and C{\'e}spedes, {Pablo F.} and G{\'o}mez, {Roberto S.} and Gisela Canedo-Marroqu{\'i}n and Riquelme, {Sebast{\'i}an A.} and Salazar-Echegarai, {Francisco J.} and Phillipe Blancou and Thomas Simon and Ignacio Anegon and Lay, {Margarita K.} and Gonz{\'a}lez, {Pablo A.} and Riedel, {Claudia A.} and Bueno, {Susan M.} and Kalergis, {Alexis M.}",
year = "2017",
month = "7",
day = "1",
doi = "10.4049/jimmunol.1601414",
language = "English",
volume = "199",
pages = "212--223",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

Espinoza, JA, León, MA, Céspedes, PF, Gómez, RS, Canedo-Marroquín, G, Riquelme, SA, Salazar-Echegarai, FJ, Blancou, P, Simon, T, Anegon, I, Lay, MK, González, PA, Riedel, CA, Bueno, SM & Kalergis, AM 2017, 'Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection', Journal of Immunology, vol. 199, n.º 1, pp. 212-223. https://doi.org/10.4049/jimmunol.1601414

Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection. / Espinoza, Janyra A.; León, Miguel A.; Céspedes, Pablo F.; Gómez, Roberto S.; Canedo-Marroquín, Gisela; Riquelme, Sebastían A.; Salazar-Echegarai, Francisco J.; Blancou, Phillipe; Simon, Thomas; Anegon, Ignacio; Lay, Margarita K.; González, Pablo A.; Riedel, Claudia A.; Bueno, Susan M.; Kalergis, Alexis M.

En: Journal of Immunology, Vol. 199, N.º 1, 01.07.2017, p. 212-223.

Resultado de la investigación: Article

TY - JOUR

T1 - Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection

AU - Espinoza, Janyra A.

AU - León, Miguel A.

AU - Céspedes, Pablo F.

AU - Gómez, Roberto S.

AU - Canedo-Marroquín, Gisela

AU - Riquelme, Sebastían A.

AU - Salazar-Echegarai, Francisco J.

AU - Blancou, Phillipe

AU - Simon, Thomas

AU - Anegon, Ignacio

AU - Lay, Margarita K.

AU - González, Pablo A.

AU - Riedel, Claudia A.

AU - Bueno, Susan M.

AU - Kalergis, Alexis M.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Human respiratory syncytial virus (hRSV) is the leading cause of severe lower respiratory tract infections in children. The development of novel prophylactic and therapeutic antiviral drugs against hRSV is imperative to control the burden of disease in the susceptible population. In this study, we examined the effects of inducing the activity of the host enzyme heme oxygenase-1 (HO-1) on hRSV replication and pathogenesis on lung inflammation induced by this virus. Our results show that after hRSV infection, HO-1 induction with metalloporphyrin cobalt protoporphyrin IX significantly reduces the loss of body weight due to hRSV-induced disease. Further, HO-1 induction also decreased viral replication and lung inflammation, as evidenced by a reduced neutrophil infiltration into the airways, with diminished cytokine and chemokine production and reduced T cell function. Concomitantly, upon cobalt protoporphyrin IX treatment, there is a significant upregulation in the production of IFN-α/β mRNAs in the lungs. Furthermore, similar antiviral and protective effects occur by inducing the expression of human HO-1 in MHC class II+ cells in transgenic mice. Finally, in vitro data suggest that HO-1 induction can modulate the susceptibility of cells, especially the airway epithelial cells, to hRSV infection.

AB - Human respiratory syncytial virus (hRSV) is the leading cause of severe lower respiratory tract infections in children. The development of novel prophylactic and therapeutic antiviral drugs against hRSV is imperative to control the burden of disease in the susceptible population. In this study, we examined the effects of inducing the activity of the host enzyme heme oxygenase-1 (HO-1) on hRSV replication and pathogenesis on lung inflammation induced by this virus. Our results show that after hRSV infection, HO-1 induction with metalloporphyrin cobalt protoporphyrin IX significantly reduces the loss of body weight due to hRSV-induced disease. Further, HO-1 induction also decreased viral replication and lung inflammation, as evidenced by a reduced neutrophil infiltration into the airways, with diminished cytokine and chemokine production and reduced T cell function. Concomitantly, upon cobalt protoporphyrin IX treatment, there is a significant upregulation in the production of IFN-α/β mRNAs in the lungs. Furthermore, similar antiviral and protective effects occur by inducing the expression of human HO-1 in MHC class II+ cells in transgenic mice. Finally, in vitro data suggest that HO-1 induction can modulate the susceptibility of cells, especially the airway epithelial cells, to hRSV infection.

UR - http://www.scopus.com/inward/record.url?scp=85021111444&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.1601414

DO - 10.4049/jimmunol.1601414

M3 - Article

AN - SCOPUS:85021111444

VL - 199

SP - 212

EP - 223

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -

Espinoza JA, León MA, Céspedes PF, Gómez RS, Canedo-Marroquín G, Riquelme SA y otros. Heme oxygenase-1 modulates human respiratory syncytial virus replication and lung pathogenesis during infection. Journal of Immunology. 2017 jul 1;199(1):212-223. https://doi.org/10.4049/jimmunol.1601414