Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element

A. Molina, E. Di Martino, J. A. Martial, M. Muller

Resultado de la investigación: Article

11 Citas (Scopus)

Resumen

We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. - 800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.

Idioma originalEnglish
Páginas (desde-hasta)353-359
Número de páginas7
PublicaciónBiochemical Journal
Volumen356
N.º2
DOI
EstadoPublished - 1 jun 2001

Huella dactilar

Tilapia
Heat-Shock Response
HSP70 Heat-Shock Proteins
Shock
Hot Temperature
Zebrafish
Embryonic Structures
Fish Proteins
Carps
Response Elements
Functional analysis
Reporter Genes
Transcription Factors
Cell culture
Cell Culture Techniques
Fish
Carcinoma
Genes
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Molina, A. ; Di Martino, E. ; Martial, J. A. ; Muller, M. / Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element. En: Biochemical Journal. 2001 ; Vol. 356, N.º 2. pp. 353-359.
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abstract = "We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. - 800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.",
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Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element. / Molina, A.; Di Martino, E.; Martial, J. A.; Muller, M.

En: Biochemical Journal, Vol. 356, N.º 2, 01.06.2001, p. 353-359.

Resultado de la investigación: Article

TY - JOUR

T1 - Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element

AU - Molina, A.

AU - Di Martino, E.

AU - Martial, J. A.

AU - Muller, M.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. - 800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.

AB - We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. - 800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.

KW - HSP70

KW - Transcription

KW - Zebrafish

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U2 - 10.1042/0264-6021:3560353

DO - 10.1042/0264-6021:3560353

M3 - Article

C2 - 11368761

AN - SCOPUS:0035366742

VL - 356

SP - 353

EP - 359

JO - Biochemical Journal

JF - Biochemical Journal

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