Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia

Valentina Vio, Ana L. Riveros, Andrea Tapia-Bustos, Carolyne Lespay-Rebolledo, Ronald Perez-Lobos, Luis Muñoz, Paola Pismante, Paola Morales, Eyleen Araya, Natalia Hassan, Mario Herrera-Marschitz, Marcelo J. Kogan

Resultado de la investigación: Contribución a una revistaArtículo

5 Citas (Scopus)

Resumen

Background: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo.

Idioma originalInglés
Páginas (desde-hasta)6839-6854
Número de páginas16
PublicaciónInternational Journal of Nanomedicine
Volumen13
DOI
EstadoPublicada - 1 ene 2018

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioingeniería
  • Biomateriales
  • Ciencias farmacéuticas
  • Descubrimiento de medicamentos
  • Química orgánica

Huella Profundice en los temas de investigación de 'Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia'. En conjunto forman una huella única.

  • Citar esto

    Vio, V., Riveros, A. L., Tapia-Bustos, A., Lespay-Rebolledo, C., Perez-Lobos, R., Muñoz, L., Pismante, P., Morales, P., Araya, E., Hassan, N., Herrera-Marschitz, M., & Kogan, M. J. (2018). Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia. International Journal of Nanomedicine, 13, 6839-6854. https://doi.org/10.2147/IJN.S175076