Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia

Valentina Vio, Ana L. Riveros, Andrea Tapia-Bustos, Carolyne Lespay-Rebolledo, Ronald Perez-Lobos, Luis Muñoz, Paola Pismante, Paola Morales, Eyleen Araya, Natalia Hassan, Mario Herrera-Marschitz, Marcelo J. Kogan

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Background: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo.

Idioma originalEnglish
Páginas (desde-hasta)6839-6854
Número de páginas16
PublicaciónInternational Journal of Nanomedicine
Volumen13
DOI
EstadoPublished - 1 ene 2018

Huella dactilar

Administrative data processing
Nanotubes
Poly(ADP-ribose) Polymerases
Asphyxia
Nanorods
Gold
Small Interfering RNA
Nanosystems
Gene Silencing
Brain
Genes
Enzyme inhibition
Adenosinetriphosphate
PC12 Cells
Gene Expression Regulation
Gene expression
DNA Repair
Peptides
Rats
Animals

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Citar esto

Vio, V., Riveros, A. L., Tapia-Bustos, A., Lespay-Rebolledo, C., Perez-Lobos, R., Muñoz, L., ... Kogan, M. J. (2018). Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia. International Journal of Nanomedicine, 13, 6839-6854. https://doi.org/10.2147/IJN.S175076
Vio, Valentina ; Riveros, Ana L. ; Tapia-Bustos, Andrea ; Lespay-Rebolledo, Carolyne ; Perez-Lobos, Ronald ; Muñoz, Luis ; Pismante, Paola ; Morales, Paola ; Araya, Eyleen ; Hassan, Natalia ; Herrera-Marschitz, Mario ; Kogan, Marcelo J. / Gold nanorods/siRNA complex administration for knockdown of PARP-1 : a potential treatment for perinatal asphyxia. En: International Journal of Nanomedicine. 2018 ; Vol. 13. pp. 6839-6854.
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title = "Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia",
abstract = "Background: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo.",
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author = "Valentina Vio and Riveros, {Ana L.} and Andrea Tapia-Bustos and Carolyne Lespay-Rebolledo and Ronald Perez-Lobos and Luis Mu{\~n}oz and Paola Pismante and Paola Morales and Eyleen Araya and Natalia Hassan and Mario Herrera-Marschitz and Kogan, {Marcelo J.}",
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Vio, V, Riveros, AL, Tapia-Bustos, A, Lespay-Rebolledo, C, Perez-Lobos, R, Muñoz, L, Pismante, P, Morales, P, Araya, E, Hassan, N, Herrera-Marschitz, M & Kogan, MJ 2018, 'Gold nanorods/siRNA complex administration for knockdown of PARP-1: a potential treatment for perinatal asphyxia', International Journal of Nanomedicine, vol. 13, pp. 6839-6854. https://doi.org/10.2147/IJN.S175076

Gold nanorods/siRNA complex administration for knockdown of PARP-1 : a potential treatment for perinatal asphyxia. / Vio, Valentina; Riveros, Ana L.; Tapia-Bustos, Andrea; Lespay-Rebolledo, Carolyne; Perez-Lobos, Ronald; Muñoz, Luis; Pismante, Paola; Morales, Paola; Araya, Eyleen; Hassan, Natalia; Herrera-Marschitz, Mario; Kogan, Marcelo J.

En: International Journal of Nanomedicine, Vol. 13, 01.01.2018, p. 6839-6854.

Resultado de la investigación: Article

TY - JOUR

T1 - Gold nanorods/siRNA complex administration for knockdown of PARP-1

T2 - a potential treatment for perinatal asphyxia

AU - Vio, Valentina

AU - Riveros, Ana L.

AU - Tapia-Bustos, Andrea

AU - Lespay-Rebolledo, Carolyne

AU - Perez-Lobos, Ronald

AU - Muñoz, Luis

AU - Pismante, Paola

AU - Morales, Paola

AU - Araya, Eyleen

AU - Hassan, Natalia

AU - Herrera-Marschitz, Mario

AU - Kogan, Marcelo J.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo.

AB - Background: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo.

KW - gold nanorods

KW - in vivo administration

KW - neonatal hypoxia

KW - PARP-1 knockdown

KW - PC12

KW - rats

KW - siRNA delivery

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U2 - 10.2147/IJN.S175076

DO - 10.2147/IJN.S175076

M3 - Article

C2 - 30498346

AN - SCOPUS:85057553985

VL - 13

SP - 6839

EP - 6854

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

ER -