Glycogen storage and muscle glucose transporters (GLUT-4) of mice selectively bred for high voluntary wheel running

Fernando R. Gomes, Enrico L. Rezende, Jessica L. Malisch, Sun K. Lee, Donato A. Rivas, Scott A. Kelly, Christian Lytle, Ben B. Yaspelkis, Theodore Garland

Resultado de la investigación: Article

39 Citas (Scopus)

Resumen

To examine the evolution of endurance-exercise behaviour, we have selectively bred four replicate lines of laboratory mice (Mus domesticus) for high voluntary wheel running ('high runner' or HR lines), while also maintaining four non-selected control (C) lines. By generation 16, HR mice ran ∼2.7-fold more than C mice, mainly by running faster (especially in females), a differential maintained through subsequent generations, suggesting an evolutionary limit of unknown origin. We hypothesized that HR mice would have higher glycogen levels before nightly running, show greater depletion of those depots during their more intense wheel running, and have increased glycogen synthase activity and GLUT-4 protein in skeletal muscle. We sampled females from generation 35 at three times (photophase 07:00 h-19:00 h) during days 5-6 of wheel access, as in the routine selection protocol: Group 1, day 5, 16:00 h-17:30 h, wheels blocked from 13:00 h; Group 2, day 6, 02:00 h-03:30 h (immediately after peak running); and Group 3, day 6, 07:00 h-08:30 h. An additional Group 4, sampled 16:00 h-17:30 h, never had wheels. HR individuals with the mini-muscle phenotype (50% reduced hindlimb muscle mass) were distinguished for statistical analyses comparing C, HR normal, and HR mini. HR mini ran more than HR normal, and at higher speeds, which might explain why they have been favored by the selective-breeding protocol. Plasma glucose was higher in Group 1 than in Group 4, indicating a training effect (phenotypic plasticity). Without wheels, no differences in gastrocnemius GLUT-4 were observed. After 5 days with wheels, all mice showed elevated GLUT-4, but HR normal and mini were 2.5-fold higher than C. At all times and irrespective of wheel access, HR mini showed approximately three-fold higher [glycogen] in gastrocnemius and altered glycogen synthase activity. HR mini also showed elevated glycogen in soleus when sampled during peak running. All mice showed some glycogen depletion during nightly wheel running, in muscles and/or liver, but the magnitude of this depletion was not large and hence does not seem to be limiting to the evolution of even-higher wheel running.

Idioma originalEnglish
Páginas (desde-hasta)238-248
Número de páginas11
PublicaciónJournal of Experimental Biology
Volumen212
N.º2
DOI
EstadoPublished - 15 ene 2009

Huella dactilar

glucose transporters
Facilitative Glucose Transport Proteins
wheels
Glycogen
Running
glycogen
glucose
muscle
breeds
Muscles
muscles
mice
fold
glycogen (starch) synthase
selective breeding
Glycogen Synthase
phenotypic plasticity
phenotype
Glucose Transporter Type 4
plasma

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Citar esto

Gomes, F. R., Rezende, E. L., Malisch, J. L., Lee, S. K., Rivas, D. A., Kelly, S. A., ... Garland, T. (2009). Glycogen storage and muscle glucose transporters (GLUT-4) of mice selectively bred for high voluntary wheel running. Journal of Experimental Biology, 212(2), 238-248. https://doi.org/10.1242/jeb.025296
Gomes, Fernando R. ; Rezende, Enrico L. ; Malisch, Jessica L. ; Lee, Sun K. ; Rivas, Donato A. ; Kelly, Scott A. ; Lytle, Christian ; Yaspelkis, Ben B. ; Garland, Theodore. / Glycogen storage and muscle glucose transporters (GLUT-4) of mice selectively bred for high voluntary wheel running. En: Journal of Experimental Biology. 2009 ; Vol. 212, N.º 2. pp. 238-248.
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abstract = "To examine the evolution of endurance-exercise behaviour, we have selectively bred four replicate lines of laboratory mice (Mus domesticus) for high voluntary wheel running ('high runner' or HR lines), while also maintaining four non-selected control (C) lines. By generation 16, HR mice ran ∼2.7-fold more than C mice, mainly by running faster (especially in females), a differential maintained through subsequent generations, suggesting an evolutionary limit of unknown origin. We hypothesized that HR mice would have higher glycogen levels before nightly running, show greater depletion of those depots during their more intense wheel running, and have increased glycogen synthase activity and GLUT-4 protein in skeletal muscle. We sampled females from generation 35 at three times (photophase 07:00 h-19:00 h) during days 5-6 of wheel access, as in the routine selection protocol: Group 1, day 5, 16:00 h-17:30 h, wheels blocked from 13:00 h; Group 2, day 6, 02:00 h-03:30 h (immediately after peak running); and Group 3, day 6, 07:00 h-08:30 h. An additional Group 4, sampled 16:00 h-17:30 h, never had wheels. HR individuals with the mini-muscle phenotype (50{\%} reduced hindlimb muscle mass) were distinguished for statistical analyses comparing C, HR normal, and HR mini. HR mini ran more than HR normal, and at higher speeds, which might explain why they have been favored by the selective-breeding protocol. Plasma glucose was higher in Group 1 than in Group 4, indicating a training effect (phenotypic plasticity). Without wheels, no differences in gastrocnemius GLUT-4 were observed. After 5 days with wheels, all mice showed elevated GLUT-4, but HR normal and mini were 2.5-fold higher than C. At all times and irrespective of wheel access, HR mini showed approximately three-fold higher [glycogen] in gastrocnemius and altered glycogen synthase activity. HR mini also showed elevated glycogen in soleus when sampled during peak running. All mice showed some glycogen depletion during nightly wheel running, in muscles and/or liver, but the magnitude of this depletion was not large and hence does not seem to be limiting to the evolution of even-higher wheel running.",
keywords = "Adaptive plasticity, Artificial selection, Experimental evolution, GLUT-4, Glycogen, Phenotypic plasticity, Selection limit, Voluntary exercise",
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Gomes, FR, Rezende, EL, Malisch, JL, Lee, SK, Rivas, DA, Kelly, SA, Lytle, C, Yaspelkis, BB & Garland, T 2009, 'Glycogen storage and muscle glucose transporters (GLUT-4) of mice selectively bred for high voluntary wheel running', Journal of Experimental Biology, vol. 212, n.º 2, pp. 238-248. https://doi.org/10.1242/jeb.025296

Glycogen storage and muscle glucose transporters (GLUT-4) of mice selectively bred for high voluntary wheel running. / Gomes, Fernando R.; Rezende, Enrico L.; Malisch, Jessica L.; Lee, Sun K.; Rivas, Donato A.; Kelly, Scott A.; Lytle, Christian; Yaspelkis, Ben B.; Garland, Theodore.

En: Journal of Experimental Biology, Vol. 212, N.º 2, 15.01.2009, p. 238-248.

Resultado de la investigación: Article

TY - JOUR

T1 - Glycogen storage and muscle glucose transporters (GLUT-4) of mice selectively bred for high voluntary wheel running

AU - Gomes, Fernando R.

AU - Rezende, Enrico L.

AU - Malisch, Jessica L.

AU - Lee, Sun K.

AU - Rivas, Donato A.

AU - Kelly, Scott A.

AU - Lytle, Christian

AU - Yaspelkis, Ben B.

AU - Garland, Theodore

PY - 2009/1/15

Y1 - 2009/1/15

N2 - To examine the evolution of endurance-exercise behaviour, we have selectively bred four replicate lines of laboratory mice (Mus domesticus) for high voluntary wheel running ('high runner' or HR lines), while also maintaining four non-selected control (C) lines. By generation 16, HR mice ran ∼2.7-fold more than C mice, mainly by running faster (especially in females), a differential maintained through subsequent generations, suggesting an evolutionary limit of unknown origin. We hypothesized that HR mice would have higher glycogen levels before nightly running, show greater depletion of those depots during their more intense wheel running, and have increased glycogen synthase activity and GLUT-4 protein in skeletal muscle. We sampled females from generation 35 at three times (photophase 07:00 h-19:00 h) during days 5-6 of wheel access, as in the routine selection protocol: Group 1, day 5, 16:00 h-17:30 h, wheels blocked from 13:00 h; Group 2, day 6, 02:00 h-03:30 h (immediately after peak running); and Group 3, day 6, 07:00 h-08:30 h. An additional Group 4, sampled 16:00 h-17:30 h, never had wheels. HR individuals with the mini-muscle phenotype (50% reduced hindlimb muscle mass) were distinguished for statistical analyses comparing C, HR normal, and HR mini. HR mini ran more than HR normal, and at higher speeds, which might explain why they have been favored by the selective-breeding protocol. Plasma glucose was higher in Group 1 than in Group 4, indicating a training effect (phenotypic plasticity). Without wheels, no differences in gastrocnemius GLUT-4 were observed. After 5 days with wheels, all mice showed elevated GLUT-4, but HR normal and mini were 2.5-fold higher than C. At all times and irrespective of wheel access, HR mini showed approximately three-fold higher [glycogen] in gastrocnemius and altered glycogen synthase activity. HR mini also showed elevated glycogen in soleus when sampled during peak running. All mice showed some glycogen depletion during nightly wheel running, in muscles and/or liver, but the magnitude of this depletion was not large and hence does not seem to be limiting to the evolution of even-higher wheel running.

AB - To examine the evolution of endurance-exercise behaviour, we have selectively bred four replicate lines of laboratory mice (Mus domesticus) for high voluntary wheel running ('high runner' or HR lines), while also maintaining four non-selected control (C) lines. By generation 16, HR mice ran ∼2.7-fold more than C mice, mainly by running faster (especially in females), a differential maintained through subsequent generations, suggesting an evolutionary limit of unknown origin. We hypothesized that HR mice would have higher glycogen levels before nightly running, show greater depletion of those depots during their more intense wheel running, and have increased glycogen synthase activity and GLUT-4 protein in skeletal muscle. We sampled females from generation 35 at three times (photophase 07:00 h-19:00 h) during days 5-6 of wheel access, as in the routine selection protocol: Group 1, day 5, 16:00 h-17:30 h, wheels blocked from 13:00 h; Group 2, day 6, 02:00 h-03:30 h (immediately after peak running); and Group 3, day 6, 07:00 h-08:30 h. An additional Group 4, sampled 16:00 h-17:30 h, never had wheels. HR individuals with the mini-muscle phenotype (50% reduced hindlimb muscle mass) were distinguished for statistical analyses comparing C, HR normal, and HR mini. HR mini ran more than HR normal, and at higher speeds, which might explain why they have been favored by the selective-breeding protocol. Plasma glucose was higher in Group 1 than in Group 4, indicating a training effect (phenotypic plasticity). Without wheels, no differences in gastrocnemius GLUT-4 were observed. After 5 days with wheels, all mice showed elevated GLUT-4, but HR normal and mini were 2.5-fold higher than C. At all times and irrespective of wheel access, HR mini showed approximately three-fold higher [glycogen] in gastrocnemius and altered glycogen synthase activity. HR mini also showed elevated glycogen in soleus when sampled during peak running. All mice showed some glycogen depletion during nightly wheel running, in muscles and/or liver, but the magnitude of this depletion was not large and hence does not seem to be limiting to the evolution of even-higher wheel running.

KW - Adaptive plasticity

KW - Artificial selection

KW - Experimental evolution

KW - GLUT-4

KW - Glycogen

KW - Phenotypic plasticity

KW - Selection limit

KW - Voluntary exercise

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