Resumen
In the present study, we have examined the physiological properties of synaptic currents mediated by GlyRs and GABAARs after culturing spinal neurons with a gephyrin antisense oligonucleotide. Application of gephyrin antisense, but not the sense, reduced the glycinergic mIPSC amplitude (∼50%) and frequency (∼85%), indicating the importance of gephyrin for GlyR anchoring at postsynaptic sites. On the other hand, the glycine-evoked current amplitude was unchanged indicating that functional GlyRs were still located in the extrasynaptic membrane. The analysis of the GABAergic transmission in the same neurons revealed ∼70% reduction in the frequency of the GABAergic mIPSCs, without changes in the amplitude. Interestingly, the modulation of remaining GABAAR-mediated synaptic events by zinc and diazepam was significantly altered by the antisense. These results indicate that gephyrin is required for the membrane insertion/stabilization of the GABA AR γ2 subunit as well as for its subsequent localization in the postsynaptic membrane.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 40-47 |
| Número de páginas | 8 |
| Publicación | Brain Research |
| Volumen | 1050 |
| N.º | 1-2 |
| DOI | |
| Estado | Published - 19 jul 2005 |
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ASJC Scopus subject areas
- Neuroscience(all)
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Glycinergic and GABAergic synaptic transmission are differentially affected by gephyrin in spinal neurons. / Van Zundert, Brigitte; Castro, Patricio; Aguayo, Luis G.
En: Brain Research, Vol. 1050, N.º 1-2, 19.07.2005, p. 40-47.Resultado de la investigación: Article
TY - JOUR
T1 - Glycinergic and GABAergic synaptic transmission are differentially affected by gephyrin in spinal neurons
AU - Van Zundert, Brigitte
AU - Castro, Patricio
AU - Aguayo, Luis G.
PY - 2005/7/19
Y1 - 2005/7/19
N2 - In the present study, we have examined the physiological properties of synaptic currents mediated by GlyRs and GABAARs after culturing spinal neurons with a gephyrin antisense oligonucleotide. Application of gephyrin antisense, but not the sense, reduced the glycinergic mIPSC amplitude (∼50%) and frequency (∼85%), indicating the importance of gephyrin for GlyR anchoring at postsynaptic sites. On the other hand, the glycine-evoked current amplitude was unchanged indicating that functional GlyRs were still located in the extrasynaptic membrane. The analysis of the GABAergic transmission in the same neurons revealed ∼70% reduction in the frequency of the GABAergic mIPSCs, without changes in the amplitude. Interestingly, the modulation of remaining GABAAR-mediated synaptic events by zinc and diazepam was significantly altered by the antisense. These results indicate that gephyrin is required for the membrane insertion/stabilization of the GABA AR γ2 subunit as well as for its subsequent localization in the postsynaptic membrane.
AB - In the present study, we have examined the physiological properties of synaptic currents mediated by GlyRs and GABAARs after culturing spinal neurons with a gephyrin antisense oligonucleotide. Application of gephyrin antisense, but not the sense, reduced the glycinergic mIPSC amplitude (∼50%) and frequency (∼85%), indicating the importance of gephyrin for GlyR anchoring at postsynaptic sites. On the other hand, the glycine-evoked current amplitude was unchanged indicating that functional GlyRs were still located in the extrasynaptic membrane. The analysis of the GABAergic transmission in the same neurons revealed ∼70% reduction in the frequency of the GABAergic mIPSCs, without changes in the amplitude. Interestingly, the modulation of remaining GABAAR-mediated synaptic events by zinc and diazepam was significantly altered by the antisense. These results indicate that gephyrin is required for the membrane insertion/stabilization of the GABA AR γ2 subunit as well as for its subsequent localization in the postsynaptic membrane.
KW - Benzodiazepine
KW - GABAergic
KW - Gephyrin
KW - Glycinergic
KW - mIPSCs
KW - Strychnine
UR - http://www.scopus.com/inward/record.url?scp=21544482578&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2005.05.014
DO - 10.1016/j.brainres.2005.05.014
M3 - Article
C2 - 15963957
AN - SCOPUS:21544482578
VL - 1050
SP - 40
EP - 47
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -