Glycinergic and GABAergic synaptic transmission are differentially affected by gephyrin in spinal neurons

Brigitte Van Zundert, Patricio Castro, Luis G. Aguayo

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

In the present study, we have examined the physiological properties of synaptic currents mediated by GlyRs and GABAARs after culturing spinal neurons with a gephyrin antisense oligonucleotide. Application of gephyrin antisense, but not the sense, reduced the glycinergic mIPSC amplitude (∼50%) and frequency (∼85%), indicating the importance of gephyrin for GlyR anchoring at postsynaptic sites. On the other hand, the glycine-evoked current amplitude was unchanged indicating that functional GlyRs were still located in the extrasynaptic membrane. The analysis of the GABAergic transmission in the same neurons revealed ∼70% reduction in the frequency of the GABAergic mIPSCs, without changes in the amplitude. Interestingly, the modulation of remaining GABAAR-mediated synaptic events by zinc and diazepam was significantly altered by the antisense. These results indicate that gephyrin is required for the membrane insertion/stabilization of the GABA AR γ2 subunit as well as for its subsequent localization in the postsynaptic membrane.

Idioma originalEnglish
Páginas (desde-hasta)40-47
Número de páginas8
PublicaciónBrain Research
Volumen1050
N.º1-2
DOI
EstadoPublished - 19 jul 2005

Huella dactilar

Synaptic Transmission
Neurons
Membranes
Antisense Oligonucleotides
Diazepam
Glycine
gamma-Aminobutyric Acid
Zinc
gephyrin

ASJC Scopus subject areas

  • Neuroscience(all)

Citar esto

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abstract = "In the present study, we have examined the physiological properties of synaptic currents mediated by GlyRs and GABAARs after culturing spinal neurons with a gephyrin antisense oligonucleotide. Application of gephyrin antisense, but not the sense, reduced the glycinergic mIPSC amplitude (∼50{\%}) and frequency (∼85{\%}), indicating the importance of gephyrin for GlyR anchoring at postsynaptic sites. On the other hand, the glycine-evoked current amplitude was unchanged indicating that functional GlyRs were still located in the extrasynaptic membrane. The analysis of the GABAergic transmission in the same neurons revealed ∼70{\%} reduction in the frequency of the GABAergic mIPSCs, without changes in the amplitude. Interestingly, the modulation of remaining GABAAR-mediated synaptic events by zinc and diazepam was significantly altered by the antisense. These results indicate that gephyrin is required for the membrane insertion/stabilization of the GABA AR γ2 subunit as well as for its subsequent localization in the postsynaptic membrane.",
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Glycinergic and GABAergic synaptic transmission are differentially affected by gephyrin in spinal neurons. / Van Zundert, Brigitte; Castro, Patricio; Aguayo, Luis G.

En: Brain Research, Vol. 1050, N.º 1-2, 19.07.2005, p. 40-47.

Resultado de la investigación: Article

TY - JOUR

T1 - Glycinergic and GABAergic synaptic transmission are differentially affected by gephyrin in spinal neurons

AU - Van Zundert, Brigitte

AU - Castro, Patricio

AU - Aguayo, Luis G.

PY - 2005/7/19

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KW - Benzodiazepine

KW - GABAergic

KW - Gephyrin

KW - Glycinergic

KW - mIPSCs

KW - Strychnine

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