Glycine receptors involved in synaptic transmission are selectively regulated by the cytoskeleton in mouse spinal neurons

Brigitte Van Zundert, Francisco J. Alvarez, Gonzalo E. Yevenes, Juan G. Cárcamo, Juan Carlos Vera, Luis G. Aguayo

Resultado de la investigación: Contribución a una revistaArtículo

20 Citas (Scopus)

Resumen

Using whole cell patchclamp recordings, we examined the effect of colchicine, a microtubule disrupter, on the properties of glycine receptors (GlyRs) in cultured spinal cord neurons. Confocal microscopy revealed that colchicine treatment effectively altered microtubule bundles and neuronal morphology. Application of colchicine via the culture media or the patchpipette, however, did not affect the whole cell current rundown (73 ± 6% of control after 1 h), the sensitivity of the GlyR to glycine (EC50 = 29 ± 1 μM), or strychnine inhibition (47 ± 5% of control after 100 nM strychnine). On the other hand, colchicine dialyzed for 25 min via the patch pipette selectively reduced the quantal amplitude of spontaneous glycinergic miniature inhibitory postsynaptic currents (mIP-SCs) to 68 ± 5% of control. This effect was specific for GlyRs since synaptic events mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and GABAA receptors were unchanged. In conclusion, this study indicates that microtubules can regulate the function of GlyRs involved in inhibitory synaptic transmission.

Idioma originalInglés
Páginas (desde-hasta)640-644
Número de páginas5
PublicaciónJournal of Neurophysiology
Volumen87
N.º1
DOI
EstadoPublicada - 1 ene 2002

Áreas temáticas de ASJC Scopus

  • Neurociencia (todo)
  • Fisiología

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