Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans

María Cristina Ravanal, Yeison Espinosa, Lorena Rosa, Inmaculada Vaca, Rubén Polanco, Jaime Eyzaguirre, Gloria Levicán, Renato Chávez

Resultado de la investigación: Contribución a una revistaArtículo

4 Citas (Scopus)

Resumen

The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0. 72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.

Idioma originalInglés
Páginas (desde-hasta)152-155
Número de páginas4
PublicaciónMycoscience
Volumen53
N.º2
DOI
EstadoPublicada - mar 2012

Áreas temáticas de ASJC Scopus

  • Ecología, evolución, comportamiento y sistemática

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