Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans

María Cristina Ravanal, Yeison Espinosa, Lorena Rosa, Inmaculada Vaca, Rubén Polanco, Jaime Eyzaguirre, Gloria Levicán, Renato Chávez

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0. 72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.

Idioma originalEnglish
Páginas (desde-hasta)152-155
Número de páginas4
PublicaciónMycoscience
Volumen53
N.º2
DOI
EstadoPublished - 1 ene 2012

Huella dactilar

Talaromyces purpurogenus
Aspergillus nidulans
xylanases
clone
glucose
clones
plasmid
secretion
peptide
amplification
transform
glyceraldehyde-3-phosphate dehydrogenase
phosphate
signal peptide
Penicillium
plasmids
promoter regions

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Citar esto

Ravanal, María Cristina ; Espinosa, Yeison ; Rosa, Lorena ; Vaca, Inmaculada ; Polanco, Rubén ; Eyzaguirre, Jaime ; Levicán, Gloria ; Chávez, Renato. / Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans. En: Mycoscience. 2012 ; Vol. 53, N.º 2. pp. 152-155.
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abstract = "The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0. 72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.",
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Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans. / Ravanal, María Cristina; Espinosa, Yeison; Rosa, Lorena; Vaca, Inmaculada; Polanco, Rubén; Eyzaguirre, Jaime; Levicán, Gloria; Chávez, Renato.

En: Mycoscience, Vol. 53, N.º 2, 01.01.2012, p. 152-155.

Resultado de la investigación: Article

TY - JOUR

T1 - Glucose-induced production of a Penicillium purpurogenum xylanase by Aspergillus nidulans

AU - Ravanal, María Cristina

AU - Espinosa, Yeison

AU - Rosa, Lorena

AU - Vaca, Inmaculada

AU - Polanco, Rubén

AU - Eyzaguirre, Jaime

AU - Levicán, Gloria

AU - Chávez, Renato

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0. 72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.

AB - The heterologous secretion of xylanase B from Penicillium purpurogenum using glucose as inducer was performed in Aspergillus nidulans. For this purpose, plasmid pEVXB, containing the xylanase B cDNA (including its own signal peptide) under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter, was constructed and used to transform A. nidulans. Analysis of transformed clones showed that several of them secreted extracellular xylanase activity when grown in a medium containing glucose. The clone showing the highest xylanase activity was chosen for further work. When this clone was grown on glucose, xylanase activity (0. 72 U/ml), was detected in the culture supernatant. This was confirmed by a zymogram analysis and by the amplification of xynB cDNA from this clone. To our knowledge, this is the first example of the production of a xylanase from Penicillium in heterologous fungal hosts using glucose as inducer.

KW - Glucose induction

KW - Heterologous expression

KW - Penicillium purpurogenum

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