Glucocorticoid regulation of the vitamin D receptor

Alejandro A. Hidalgo, Donald L. Trump, Candace S. Johnson

Resultado de la investigación: Article

23 Citas (Scopus)

Resumen

Many studies indicate calcitriol has potent anti-tumor activity in different types of cancers. However, high levels of vitamin D can produce hypercalcemia in some patients. Glucocorticoids are used to ameliorate hypercalcemia and to enhance calcitriol anti-tumor activity. Calcitriol in combination with the glucocorticoid dexamethasone (Dex) increased vitamin D receptor (VDR) protein levels and ligand binding in squamous cell carcinoma VII (SCC). In this study we found that both calcitriol and Dex induce VDR- and glucocorticoid receptor (GR)-mediated transcription respectively, indicating both hormone receptors are active in SCC. Pre-treatment with Dex increases VDR-mediated transcription at the human CYP24A1 promoter. Whereas, pre-treatment with other steroid hormones, including dihydrotestosterone and R1881, has no effect on VDR-mediated transcription. Real-time PCR indicates treatment with Dex increases Vdr transcripts in a time-dependent manner, suggesting Dex may directly regulate expression of Vdr. Numerous putative glucocorticoid response elements (GREs) were found in the Vdr gene. Chromatin immuno-precipitation (ChIP) assay demonstrated GR binding at several putative GREs located within the mouse Vdr gene. However, none of the putative GREs studied increase GR-mediated transcription in luciferase reporter assays. In an attempt to identify the response element responsible for Vdr transcript regulation, future studies will continue to analyze newly identified GREs more distal from the Vdr gene promoter.

Idioma originalEnglish
Páginas (desde-hasta)372-375
Número de páginas4
PublicaciónJournal of Steroid Biochemistry and Molecular Biology
Volumen121
N.º1-2
DOI
EstadoPublished - 1 ene 2010

Huella dactilar

Calcitriol Receptors
Glucocorticoids
Response Elements
Dexamethasone
Calcitriol
Transcription
Glucocorticoid Receptors
Genes
Hypercalcemia
Tumors
Squamous Cell Carcinoma
Assays
Steroid hormones
Metribolone
Hormones
Neoplasms
Dihydrotestosterone
Luciferases
Vitamin D
Chromatin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Citar esto

Hidalgo, Alejandro A. ; Trump, Donald L. ; Johnson, Candace S. / Glucocorticoid regulation of the vitamin D receptor. En: Journal of Steroid Biochemistry and Molecular Biology. 2010 ; Vol. 121, N.º 1-2. pp. 372-375.
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Glucocorticoid regulation of the vitamin D receptor. / Hidalgo, Alejandro A.; Trump, Donald L.; Johnson, Candace S.

En: Journal of Steroid Biochemistry and Molecular Biology, Vol. 121, N.º 1-2, 01.01.2010, p. 372-375.

Resultado de la investigación: Article

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