Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli

Roberto C. Molina-Quiroz, David E. Loyola, Waldo A. Díaz-Vásquez, Felipe A. Arenas, Ulises Urzúa, José M. Pérez-Donoso, Claudio C. Vásquez

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.

Idioma originalEnglish
Páginas (desde-hasta)566-570
Número de páginas5
PublicaciónResearch in Microbiology
Volumen165
N.º7
DOI
EstadoPublished - 1 ene 2014

Huella dactilar

Anaerobiosis
Escherichia coli
Respiration
Oxidative Stress
Protein Folding
Microarray Analysis
Heat-Shock Proteins
Oligonucleotide Array Sequence Analysis
Gram-Negative Bacteria
Sulfhydryl Compounds
Oxygen Consumption
Cell Wall
Genes
Oxidation-Reduction
Up-Regulation
Down-Regulation
Oxygen
Enzymes
tellurous acid

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Citar esto

Molina-Quiroz, R. C., Loyola, D. E., Díaz-Vásquez, W. A., Arenas, F. A., Urzúa, U., Pérez-Donoso, J. M., & Vásquez, C. C. (2014). Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli. Research in Microbiology, 165(7), 566-570. https://doi.org/10.1016/j.resmic.2014.07.003
Molina-Quiroz, Roberto C. ; Loyola, David E. ; Díaz-Vásquez, Waldo A. ; Arenas, Felipe A. ; Urzúa, Ulises ; Pérez-Donoso, José M. ; Vásquez, Claudio C. / Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli. En: Research in Microbiology. 2014 ; Vol. 165, N.º 7. pp. 566-570.
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abstract = "Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.",
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Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli. / Molina-Quiroz, Roberto C.; Loyola, David E.; Díaz-Vásquez, Waldo A.; Arenas, Felipe A.; Urzúa, Ulises; Pérez-Donoso, José M.; Vásquez, Claudio C.

En: Research in Microbiology, Vol. 165, N.º 7, 01.01.2014, p. 566-570.

Resultado de la investigación: Article

TY - JOUR

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AU - Molina-Quiroz, Roberto C.

AU - Loyola, David E.

AU - Díaz-Vásquez, Waldo A.

AU - Arenas, Felipe A.

AU - Urzúa, Ulises

AU - Pérez-Donoso, José M.

AU - Vásquez, Claudio C.

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Y1 - 2014/1/1

N2 - Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.

AB - Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corroborated that tellurite-exposed cells effectively consume oxygen at lower rates than untreated controls.

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