Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa

Luis Pastenes, Camilo Valdivieso, Alex Di Genova, Dante Travisany, Andrew Hart, Martín Montecino, Ariel Orellana, Mauricio Gonzalez, Rodrigo A. Gutiérrez, Miguel L. Allende, Alejandro Maass, Marco A. Méndez

Resultado de la investigación: Article

3 Citas (Scopus)

Resumen

The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 °C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.

Idioma originalEnglish
Número de artículo1966
PublicaciónScientific Reports
Volumen7
N.º1
DOI
EstadoPublished - 1 dic 2017

Huella dactilar

toads
tadpoles
gene expression
transcriptome
temperature
genes
bioinformatics
metamorphosis
seasonal variation
life history
Rhinella
developmental stages
RNA
genetic variation
habitats
animals

ASJC Scopus subject areas

  • General

Citar esto

Pastenes, Luis ; Valdivieso, Camilo ; Di Genova, Alex ; Travisany, Dante ; Hart, Andrew ; Montecino, Martín ; Orellana, Ariel ; Gonzalez, Mauricio ; Gutiérrez, Rodrigo A. ; Allende, Miguel L. ; Maass, Alejandro ; Méndez, Marco A. / Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa. En: Scientific Reports. 2017 ; Vol. 7, N.º 1.
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abstract = "The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 °C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.",
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Pastenes, L, Valdivieso, C, Di Genova, A, Travisany, D, Hart, A, Montecino, M, Orellana, A, Gonzalez, M, Gutiérrez, RA, Allende, ML, Maass, A & Méndez, MA 2017, 'Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa', Scientific Reports, vol. 7, n.º 1, 1966. https://doi.org/10.1038/s41598-017-01982-z

Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa. / Pastenes, Luis; Valdivieso, Camilo; Di Genova, Alex; Travisany, Dante; Hart, Andrew; Montecino, Martín; Orellana, Ariel; Gonzalez, Mauricio; Gutiérrez, Rodrigo A.; Allende, Miguel L.; Maass, Alejandro; Méndez, Marco A.

En: Scientific Reports, Vol. 7, N.º 1, 1966, 01.12.2017.

Resultado de la investigación: Article

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AU - Pastenes, Luis

AU - Valdivieso, Camilo

AU - Di Genova, Alex

AU - Travisany, Dante

AU - Hart, Andrew

AU - Montecino, Martín

AU - Orellana, Ariel

AU - Gonzalez, Mauricio

AU - Gutiérrez, Rodrigo A.

AU - Allende, Miguel L.

AU - Maass, Alejandro

AU - Méndez, Marco A.

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N2 - The anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 °C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.

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