Germination response of spores of the pathogenic bacterium Clostridium perfringens and Clostridium difficile to cultured human epithelial cells

Daniel Paredes-Sabja, Mahfuzur R. Sarker

Resultado de la investigación: Article

25 Citas (Scopus)

Resumen

Spores of pathogenic Clostridium perfringens and Clostridium difficile must germinate in the food vehicle and/or host's intestinal tract to cause disease. In this work, we examined the germination response of spores of C. perfringens and C. difficile upon incubation with cultured human epithelial cell lines (Caco-2, HeLa and HT-29). C. perfringens spores of various sources were able to germinate to different extents; while spores of a non-food-borne isolate germinated very well, spores of food-borne and animal isolates germinated poorly in human epithelial cells. In contrast, no detectable spore germination (i.e., loss of spore heat resistance) was observed upon incubation of C. difficile spores with epithelial cells; instead, there was a significant (p < 0.01) increase in heat-resistant spore titers. In C. perfringens, the highest spore germination response observed with the HT-29 cell line, might be in part, due to the expression of germination factor with peptidoglycan cortex hydrolysis activity by HT-29 cells. Collectively, these findings might well have implications in understanding the mechanism of clostridial spore germination in vivo.

Idioma originalEnglish
Páginas (desde-hasta)78-84
Número de páginas7
PublicaciónAnaerobe
Volumen17
N.º2
DOI
EstadoPublished - 1 abr 2011

Huella dactilar

Clostridium perfringens
Clostridium difficile
Germination
Spores
Epithelial Cells
Bacteria
HT29 Cells
Hot Temperature
Cell Line
Food
Peptidoglycan
Hydrolysis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Citar esto

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