Genotyping and Persistence of Candida albicans from Pregnant Women with Vulvovaginal Candidiasis

Cecilia V. Tapia, Germán Hermosilla, Paula Fortes, Claudio Alburquenque, Sergio Bucarey, Hugo Salinas, Paula I. Rodas, María Cristina Díaz, Fabien Magne

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

13 Citas (Scopus)

Resumen

Objective: To study Candida albicans genotypes using RAPD and their susceptibility to fluconazole in healthy pregnant women and in vulvovaginal candidiasis (VVC) patients after topical treatment with clotrimazole. Methods: Vaginal swabs were collected at t = 0 and t = 1 (1 month later) in pregnant women (control group, n = 33), and before (t = 0), at 1 month (t = 1) and at 2 months (t = 2) after clotrimazole treatment in pregnant women with VVC. Results: Candida albicans was isolated in 30% of healthy pregnant women and 80% of patients with VVC. A high genetic heterogeneity was observed in C. albicans genotypes between individuals. In patients with VVC, topical antifungal treatment with clotrimazole was clinically effective, but only in a 62% C. albicans was eradicated. In patients in which C. albicans was not eradicated, this microorganism persisted for 1 or 2 months after the antifungal treatment. The persistent colonies were not associated with a specific genotype, but they were associated with higher MICs in comparison with colonies isolated from the control group. Conclusions: Therapy with topical clotrimazole, despite a good clinical outcome, could not eradicate completely C. albicans allowing the persistence of genotypes, with higher MICs to fluconazole. More studies with higher number of patients are needed to validate this preliminary finding.

Idioma originalInglés
Páginas (desde-hasta)339-347
Número de páginas9
PublicaciónMycopathologia
Volumen182
N.º3-4
DOI
EstadoPublicada - 1 abr. 2017

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Microbiología y biotecnología aplicadas
  • Agronomía y cultivos
  • Veterinaria (miscelánea)

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