Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile

Viviana Toledo, Henk C. Den Bakker, Juan Carlos Hormazábal, Gerardo González-Rocha, Helia Bello-Toledo, Magaly Toro, Andrea I. Moreno-Switt

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

Listeria monocytogenes is the causative agent of listeriosis, which is an uncommon but severe infection associated with high mortality rates in humans especially in high-risk groups. This bacterium survives a variety of stress conditions (e.g., high osmolality, low pH), which allows it to colonize different niches especially niches found in food processing environments. Additionally, a considerable heterogeneity in pathogenic potential has been observed in different strains. In this study, 38 isolates of L. monocytogenes collected in Chile from clinical samples (n = 22) and non-clinical samples (n = 16) were analyzed using whole genome sequencing (WGS) to determine their genomic diversity. A core genome Single Nucleotide Polymorphism (SNP) tree using 55 additional L. monocytogenes accessions classified the Chilean isolates in lineages I (n = 25) and II (n = 13). In silico, Multi-locus sequence typing (MLST) differentiated the isolates into 13 sequence types (ST) in which the most common were ST1 (15 isolates) and ST9 (6 isolates) and represented 55% of the isolates. Genomic elements associated with virulence (i.e., LIPI-1, LIPI-3, inlA, inlB, inlC, inlG, inlH, inlD, inlE, inlK, inlF, and inlJ) and stress survival (i.e., stress survival islet 1 and stress survival islet 2) were unevenly distributed among clinical and non-clinical isolates. In addition, one novel inlA premature stop codon (PMSC) was detected. Comparative analysis of L. monocytogenes circulating in Chile revealed the presence of globally distributed sequence types along with differences among the isolates analyzed at a genomic level specifically associated with virulence and stress survival.

Idioma originalEnglish
Número de artículo396
PublicaciónGenes
Volumen9
N.º8
DOI
EstadoPublished - 2 ago 2018

Huella dactilar

Chile
Listeria monocytogenes
Survival
Virulence
Genome
Listeriosis
Food Handling
Nonsense Codon
Computer Simulation
Osmolar Concentration
Single Nucleotide Polymorphism
Bacteria
Mortality
Infection

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Citar esto

Toledo, V., Den Bakker, H. C., Hormazábal, J. C., González-Rocha, G., Bello-Toledo, H., Toro, M., & Moreno-Switt, A. I. (2018). Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile. Genes, 9(8), [396]. https://doi.org/10.3390/genes9080396
Toledo, Viviana ; Den Bakker, Henk C. ; Hormazábal, Juan Carlos ; González-Rocha, Gerardo ; Bello-Toledo, Helia ; Toro, Magaly ; Moreno-Switt, Andrea I. / Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile. En: Genes. 2018 ; Vol. 9, N.º 8.
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abstract = "Listeria monocytogenes is the causative agent of listeriosis, which is an uncommon but severe infection associated with high mortality rates in humans especially in high-risk groups. This bacterium survives a variety of stress conditions (e.g., high osmolality, low pH), which allows it to colonize different niches especially niches found in food processing environments. Additionally, a considerable heterogeneity in pathogenic potential has been observed in different strains. In this study, 38 isolates of L. monocytogenes collected in Chile from clinical samples (n = 22) and non-clinical samples (n = 16) were analyzed using whole genome sequencing (WGS) to determine their genomic diversity. A core genome Single Nucleotide Polymorphism (SNP) tree using 55 additional L. monocytogenes accessions classified the Chilean isolates in lineages I (n = 25) and II (n = 13). In silico, Multi-locus sequence typing (MLST) differentiated the isolates into 13 sequence types (ST) in which the most common were ST1 (15 isolates) and ST9 (6 isolates) and represented 55{\%} of the isolates. Genomic elements associated with virulence (i.e., LIPI-1, LIPI-3, inlA, inlB, inlC, inlG, inlH, inlD, inlE, inlK, inlF, and inlJ) and stress survival (i.e., stress survival islet 1 and stress survival islet 2) were unevenly distributed among clinical and non-clinical isolates. In addition, one novel inlA premature stop codon (PMSC) was detected. Comparative analysis of L. monocytogenes circulating in Chile revealed the presence of globally distributed sequence types along with differences among the isolates analyzed at a genomic level specifically associated with virulence and stress survival.",
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Toledo, V, Den Bakker, HC, Hormazábal, JC, González-Rocha, G, Bello-Toledo, H, Toro, M & Moreno-Switt, AI 2018, 'Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile', Genes, vol. 9, n.º 8, 396. https://doi.org/10.3390/genes9080396

Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile. / Toledo, Viviana; Den Bakker, Henk C.; Hormazábal, Juan Carlos; González-Rocha, Gerardo; Bello-Toledo, Helia; Toro, Magaly; Moreno-Switt, Andrea I.

En: Genes, Vol. 9, N.º 8, 396, 02.08.2018.

Resultado de la investigación: Article

TY - JOUR

T1 - Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile

AU - Toledo, Viviana

AU - Den Bakker, Henk C.

AU - Hormazábal, Juan Carlos

AU - González-Rocha, Gerardo

AU - Bello-Toledo, Helia

AU - Toro, Magaly

AU - Moreno-Switt, Andrea I.

PY - 2018/8/2

Y1 - 2018/8/2

N2 - Listeria monocytogenes is the causative agent of listeriosis, which is an uncommon but severe infection associated with high mortality rates in humans especially in high-risk groups. This bacterium survives a variety of stress conditions (e.g., high osmolality, low pH), which allows it to colonize different niches especially niches found in food processing environments. Additionally, a considerable heterogeneity in pathogenic potential has been observed in different strains. In this study, 38 isolates of L. monocytogenes collected in Chile from clinical samples (n = 22) and non-clinical samples (n = 16) were analyzed using whole genome sequencing (WGS) to determine their genomic diversity. A core genome Single Nucleotide Polymorphism (SNP) tree using 55 additional L. monocytogenes accessions classified the Chilean isolates in lineages I (n = 25) and II (n = 13). In silico, Multi-locus sequence typing (MLST) differentiated the isolates into 13 sequence types (ST) in which the most common were ST1 (15 isolates) and ST9 (6 isolates) and represented 55% of the isolates. Genomic elements associated with virulence (i.e., LIPI-1, LIPI-3, inlA, inlB, inlC, inlG, inlH, inlD, inlE, inlK, inlF, and inlJ) and stress survival (i.e., stress survival islet 1 and stress survival islet 2) were unevenly distributed among clinical and non-clinical isolates. In addition, one novel inlA premature stop codon (PMSC) was detected. Comparative analysis of L. monocytogenes circulating in Chile revealed the presence of globally distributed sequence types along with differences among the isolates analyzed at a genomic level specifically associated with virulence and stress survival.

AB - Listeria monocytogenes is the causative agent of listeriosis, which is an uncommon but severe infection associated with high mortality rates in humans especially in high-risk groups. This bacterium survives a variety of stress conditions (e.g., high osmolality, low pH), which allows it to colonize different niches especially niches found in food processing environments. Additionally, a considerable heterogeneity in pathogenic potential has been observed in different strains. In this study, 38 isolates of L. monocytogenes collected in Chile from clinical samples (n = 22) and non-clinical samples (n = 16) were analyzed using whole genome sequencing (WGS) to determine their genomic diversity. A core genome Single Nucleotide Polymorphism (SNP) tree using 55 additional L. monocytogenes accessions classified the Chilean isolates in lineages I (n = 25) and II (n = 13). In silico, Multi-locus sequence typing (MLST) differentiated the isolates into 13 sequence types (ST) in which the most common were ST1 (15 isolates) and ST9 (6 isolates) and represented 55% of the isolates. Genomic elements associated with virulence (i.e., LIPI-1, LIPI-3, inlA, inlB, inlC, inlG, inlH, inlD, inlE, inlK, inlF, and inlJ) and stress survival (i.e., stress survival islet 1 and stress survival islet 2) were unevenly distributed among clinical and non-clinical isolates. In addition, one novel inlA premature stop codon (PMSC) was detected. Comparative analysis of L. monocytogenes circulating in Chile revealed the presence of globally distributed sequence types along with differences among the isolates analyzed at a genomic level specifically associated with virulence and stress survival.

KW - Chile

KW - Genomic diversity

KW - Listeria monocytogenes

KW - Single nucleotide polymorphism

KW - Whole genome sequencing

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U2 - 10.3390/genes9080396

DO - 10.3390/genes9080396

M3 - Article

VL - 9

JO - Genes

JF - Genes

SN - 2073-4425

IS - 8

M1 - 396

ER -

Toledo V, Den Bakker HC, Hormazábal JC, González-Rocha G, Bello-Toledo H, Toro M y otros. Genomic diversity of listeria monocytogenes isolated from clinical and non-clinical samples in Chile. Genes. 2018 ago 2;9(8). 396. https://doi.org/10.3390/genes9080396