Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

Andrés Meynard, Javier Zapata, Nicolás Salas, Claudia Betancourtt, Gabriel Pérez-Lara, Francisco Castañeda, María Eliana Ramírez, Cristian Bulboa Contador, Marie Laure Guillemin, Loretto Contreras-Porcia

Resultado de la investigación: Article

Resumen

A recent molecular taxonomic study along the Chilean coast (18° S–53° S) described 18 candidate species of bladed Bangiales of which only two were formally described. Few studies focused on local genetic and morphological diversity of bladed Bangiales and attempted to determine their intertidal distribution in contrasting habitats, and none were performed in Chile. To delimit intertidal distributions of genetic species, 66 samples of bladed Bangiales were collected at Maitencillo (32° S) in four zones: a rocky platform, a rocky wall, and two boulders zones surrounded by sandy and rocky bottoms, respectively. These samples were identified based on sequences of the mitochondrial COI and chloroplast rbcL markers. We also collected 87 specimens for morphological characterization of the most common species, rapidly assessing their putative species identity using newly developed species-diagnostic (PCR-RFLP) markers. Eight microscopic and two macroscopic morphological traits were measured. We described and named three of four species that predominate in Maitencillo (including Pyropia orbicularis): Pyropia variabilis Zapata, Meynard, Ramírez, Contreras-Porcia, sp. nov., Porphyra luchea Meynard, Ramírez, Contreras-Porcia sp. nov., and Porphyra longissima Meynard, Ramírez, Contreras-Porcia, sp. nov. With the exception of Po. longissima restricted to boulders surrounded by sandy bottom, and a morphotype of Py. variabilis restricted to rocky walls, the other species/morphotypes have overlapping intertidal distributions. Except for Po. longissima, which is clearly differentiated morphologically (longest and thinnest blades), we conclude that morphology is not sufficient to differentiate bladed Bangiales. Our findings underscore the importance of refining our knowledge of intrinsic and environmental determinants on the distribution of bladed Bangiales.

Idioma originalEnglish
PublicaciónJournal of Phycology
DOI
EstadoPublished - 1 ene 2019

Huella dactilar

Bangiales
Porphyra
Rhodophyta
Chile
new species
morphotype
refining
restriction fragment length polymorphism
chloroplasts
Pyropia
chloroplast
coasts
sampling
habitats
distribution
coast
habitat

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Citar esto

Meynard, Andrés ; Zapata, Javier ; Salas, Nicolás ; Betancourtt, Claudia ; Pérez-Lara, Gabriel ; Castañeda, Francisco ; Ramírez, María Eliana ; Bulboa Contador, Cristian ; Guillemin, Marie Laure ; Contreras-Porcia, Loretto. / Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile. En: Journal of Phycology. 2019.
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title = "Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile",
abstract = "A recent molecular taxonomic study along the Chilean coast (18° S–53° S) described 18 candidate species of bladed Bangiales of which only two were formally described. Few studies focused on local genetic and morphological diversity of bladed Bangiales and attempted to determine their intertidal distribution in contrasting habitats, and none were performed in Chile. To delimit intertidal distributions of genetic species, 66 samples of bladed Bangiales were collected at Maitencillo (32° S) in four zones: a rocky platform, a rocky wall, and two boulders zones surrounded by sandy and rocky bottoms, respectively. These samples were identified based on sequences of the mitochondrial COI and chloroplast rbcL markers. We also collected 87 specimens for morphological characterization of the most common species, rapidly assessing their putative species identity using newly developed species-diagnostic (PCR-RFLP) markers. Eight microscopic and two macroscopic morphological traits were measured. We described and named three of four species that predominate in Maitencillo (including Pyropia orbicularis): Pyropia variabilis Zapata, Meynard, Ram{\'i}rez, Contreras-Porcia, sp. nov., Porphyra luchea Meynard, Ram{\'i}rez, Contreras-Porcia sp. nov., and Porphyra longissima Meynard, Ram{\'i}rez, Contreras-Porcia, sp. nov. With the exception of Po. longissima restricted to boulders surrounded by sandy bottom, and a morphotype of Py. variabilis restricted to rocky walls, the other species/morphotypes have overlapping intertidal distributions. Except for Po. longissima, which is clearly differentiated morphologically (longest and thinnest blades), we conclude that morphology is not sufficient to differentiate bladed Bangiales. Our findings underscore the importance of refining our knowledge of intrinsic and environmental determinants on the distribution of bladed Bangiales.",
keywords = "Bangiales, Chile, COI, intertidal distribution, rbcL, taxonomy",
author = "Andr{\'e}s Meynard and Javier Zapata and Nicol{\'a}s Salas and Claudia Betancourtt and Gabriel P{\'e}rez-Lara and Francisco Casta{\~n}eda and Ram{\'i}rez, {Mar{\'i}a Eliana} and {Bulboa Contador}, Cristian and Guillemin, {Marie Laure} and Loretto Contreras-Porcia",
year = "2019",
month = "1",
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doi = "10.1111/jpy.12829",
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Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile. / Meynard, Andrés; Zapata, Javier; Salas, Nicolás; Betancourtt, Claudia; Pérez-Lara, Gabriel; Castañeda, Francisco; Ramírez, María Eliana; Bulboa Contador, Cristian; Guillemin, Marie Laure; Contreras-Porcia, Loretto.

En: Journal of Phycology, 01.01.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Genetic and morphological differentiation of Porphyra and Pyropia species (Bangiales, Rhodophyta) coexisting in a rocky intertidal in Central Chile

AU - Meynard, Andrés

AU - Zapata, Javier

AU - Salas, Nicolás

AU - Betancourtt, Claudia

AU - Pérez-Lara, Gabriel

AU - Castañeda, Francisco

AU - Ramírez, María Eliana

AU - Bulboa Contador, Cristian

AU - Guillemin, Marie Laure

AU - Contreras-Porcia, Loretto

PY - 2019/1/1

Y1 - 2019/1/1

N2 - A recent molecular taxonomic study along the Chilean coast (18° S–53° S) described 18 candidate species of bladed Bangiales of which only two were formally described. Few studies focused on local genetic and morphological diversity of bladed Bangiales and attempted to determine their intertidal distribution in contrasting habitats, and none were performed in Chile. To delimit intertidal distributions of genetic species, 66 samples of bladed Bangiales were collected at Maitencillo (32° S) in four zones: a rocky platform, a rocky wall, and two boulders zones surrounded by sandy and rocky bottoms, respectively. These samples were identified based on sequences of the mitochondrial COI and chloroplast rbcL markers. We also collected 87 specimens for morphological characterization of the most common species, rapidly assessing their putative species identity using newly developed species-diagnostic (PCR-RFLP) markers. Eight microscopic and two macroscopic morphological traits were measured. We described and named three of four species that predominate in Maitencillo (including Pyropia orbicularis): Pyropia variabilis Zapata, Meynard, Ramírez, Contreras-Porcia, sp. nov., Porphyra luchea Meynard, Ramírez, Contreras-Porcia sp. nov., and Porphyra longissima Meynard, Ramírez, Contreras-Porcia, sp. nov. With the exception of Po. longissima restricted to boulders surrounded by sandy bottom, and a morphotype of Py. variabilis restricted to rocky walls, the other species/morphotypes have overlapping intertidal distributions. Except for Po. longissima, which is clearly differentiated morphologically (longest and thinnest blades), we conclude that morphology is not sufficient to differentiate bladed Bangiales. Our findings underscore the importance of refining our knowledge of intrinsic and environmental determinants on the distribution of bladed Bangiales.

AB - A recent molecular taxonomic study along the Chilean coast (18° S–53° S) described 18 candidate species of bladed Bangiales of which only two were formally described. Few studies focused on local genetic and morphological diversity of bladed Bangiales and attempted to determine their intertidal distribution in contrasting habitats, and none were performed in Chile. To delimit intertidal distributions of genetic species, 66 samples of bladed Bangiales were collected at Maitencillo (32° S) in four zones: a rocky platform, a rocky wall, and two boulders zones surrounded by sandy and rocky bottoms, respectively. These samples were identified based on sequences of the mitochondrial COI and chloroplast rbcL markers. We also collected 87 specimens for morphological characterization of the most common species, rapidly assessing their putative species identity using newly developed species-diagnostic (PCR-RFLP) markers. Eight microscopic and two macroscopic morphological traits were measured. We described and named three of four species that predominate in Maitencillo (including Pyropia orbicularis): Pyropia variabilis Zapata, Meynard, Ramírez, Contreras-Porcia, sp. nov., Porphyra luchea Meynard, Ramírez, Contreras-Porcia sp. nov., and Porphyra longissima Meynard, Ramírez, Contreras-Porcia, sp. nov. With the exception of Po. longissima restricted to boulders surrounded by sandy bottom, and a morphotype of Py. variabilis restricted to rocky walls, the other species/morphotypes have overlapping intertidal distributions. Except for Po. longissima, which is clearly differentiated morphologically (longest and thinnest blades), we conclude that morphology is not sufficient to differentiate bladed Bangiales. Our findings underscore the importance of refining our knowledge of intrinsic and environmental determinants on the distribution of bladed Bangiales.

KW - Bangiales

KW - Chile

KW - COI

KW - intertidal distribution

KW - rbcL

KW - taxonomy

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U2 - 10.1111/jpy.12829

DO - 10.1111/jpy.12829

M3 - Article

JO - Journal of Phycology

JF - Journal of Phycology

SN - 0022-3646

ER -