Generation and analysis of expressed sequence tags from Botrytis cinerea

Evelyn Silva, Jorge Valdés, David Holmes, Amir Shmaryahu, Pablo D.T. Valenzuela

Resultado de la investigación: Article

13 Citas (Scopus)

Resumen

Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen.

Idioma originalEnglish
Páginas (desde-hasta)367-376
Número de páginas10
PublicaciónBiological Research
Volumen39
N.º2
DOI
EstadoPublished - 1 ene 2006

Huella dactilar

Botrytis
Expressed Sequence Tags
Botrytis cinerea
expressed sequence tags
unigenes
Genes
Virulence
Pathogens
Magnaporthe
Aspergillus nidulans
Neurospora crassa
genes
Fusarium
Gene Library
Cell Communication
cell communication
Saccharomyces cerevisiae
Aspergillus
Homeostasis
Metabolites

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Silva, E., Valdés, J., Holmes, D., Shmaryahu, A., & Valenzuela, P. D. T. (2006). Generation and analysis of expressed sequence tags from Botrytis cinerea. Biological Research, 39(2), 367-376. https://doi.org/10.4067/S0716-97602006000200018
Silva, Evelyn ; Valdés, Jorge ; Holmes, David ; Shmaryahu, Amir ; Valenzuela, Pablo D.T. / Generation and analysis of expressed sequence tags from Botrytis cinerea. En: Biological Research. 2006 ; Vol. 39, N.º 2. pp. 367-376.
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abstract = "Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81{\%} of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50{\%} of the sequences code for genes involved in cellular metabolism, 12{\%} for transport of metabolites, and approximately 10{\%} for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23{\%}) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22{\%} and 24{\%}, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13{\%} and 19{\%}, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen.",
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Silva, E, Valdés, J, Holmes, D, Shmaryahu, A & Valenzuela, PDT 2006, 'Generation and analysis of expressed sequence tags from Botrytis cinerea', Biological Research, vol. 39, n.º 2, pp. 367-376. https://doi.org/10.4067/S0716-97602006000200018

Generation and analysis of expressed sequence tags from Botrytis cinerea. / Silva, Evelyn; Valdés, Jorge; Holmes, David; Shmaryahu, Amir; Valenzuela, Pablo D.T.

En: Biological Research, Vol. 39, N.º 2, 01.01.2006, p. 367-376.

Resultado de la investigación: Article

TY - JOUR

T1 - Generation and analysis of expressed sequence tags from Botrytis cinerea

AU - Silva, Evelyn

AU - Valdés, Jorge

AU - Holmes, David

AU - Shmaryahu, Amir

AU - Valenzuela, Pablo D.T.

PY - 2006/1/1

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N2 - Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen.

AB - Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen.

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