Gene transfer between related bacteria by electrotransformation: Mapping Salmonella typhi genes in Salmonella typhimurium

C. S. Toro, G. C. Mora, N. Figueroa-Bossi

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

13 Citas (Scopus)

Resumen

Transfer of newly isolated mutations into a fresh background is an essential step of genetic analysis and strain construction. Gene transfer is hampered in Salmonella typhi and in other pathogenic bacteria by the lack of a generalized transduction system. We show here that this problem can be partially circumvented by using electrotransformation as a means for delivering S. typhi DNA into suitable S. typhi or Salmonella typhimurium recipients. Transferred DNA can recombine with the homologous region in the host chromosome. In one application of the method, mutations isolated in S. typhi were genetically mapped in S. typhimurium.

Idioma originalInglés
Páginas (desde-hasta)4750-4752
Número de páginas3
PublicaciónJournal of Bacteriology
Volumen180
N.º17
DOI
EstadoPublicada - 1998

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular

Huella

Profundice en los temas de investigación de 'Gene transfer between related bacteria by electrotransformation: Mapping Salmonella typhi genes in Salmonella typhimurium'. En conjunto forman una huella única.

Citar esto