Gene structure and promoter function of a teleost ribosomal protein: A tilapia (Oreochromis mossambicus) L18 gene

Alfredo Molina, Arati Iyengar, Luis F. Marins, Frédéric Biemar, Sean Hanley, Norman Maclean, Terry J. Smith, Joseph A. Martial, Marc Muller

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.

Idioma originalEnglish
Páginas (desde-hasta)195-202
Número de páginas8
PublicaciónBiochimica et Biophysica Acta - Gene Structure and Expression
Volumen1520
N.º3
DOI
EstadoPublished - 21 sep 2001

Huella dactilar

Tilapia
Genes
Tissue
Gene expression
Fish
Nucleotides
Complementary DNA
Cichlids
Zebrafish
Reporter Genes
ribosomal protein L7-L12
Fishes
Embryonic Structures
Experiments
Gene Expression

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Citar esto

Molina, Alfredo ; Iyengar, Arati ; Marins, Luis F. ; Biemar, Frédéric ; Hanley, Sean ; Maclean, Norman ; Smith, Terry J. ; Martial, Joseph A. ; Muller, Marc. / Gene structure and promoter function of a teleost ribosomal protein : A tilapia (Oreochromis mossambicus) L18 gene. En: Biochimica et Biophysica Acta - Gene Structure and Expression. 2001 ; Vol. 1520, N.º 3. pp. 195-202.
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abstract = "We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.",
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Gene structure and promoter function of a teleost ribosomal protein : A tilapia (Oreochromis mossambicus) L18 gene. / Molina, Alfredo; Iyengar, Arati; Marins, Luis F.; Biemar, Frédéric; Hanley, Sean; Maclean, Norman; Smith, Terry J.; Martial, Joseph A.; Muller, Marc.

En: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1520, N.º 3, 21.09.2001, p. 195-202.

Resultado de la investigación: Article

TY - JOUR

T1 - Gene structure and promoter function of a teleost ribosomal protein

T2 - A tilapia (Oreochromis mossambicus) L18 gene

AU - Molina, Alfredo

AU - Iyengar, Arati

AU - Marins, Luis F.

AU - Biemar, Frédéric

AU - Hanley, Sean

AU - Maclean, Norman

AU - Smith, Terry J.

AU - Martial, Joseph A.

AU - Muller, Marc

PY - 2001/9/21

Y1 - 2001/9/21

N2 - We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.

AB - We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.

KW - Fish

KW - L18

KW - Promoter

KW - Ribosomal protein

KW - Tilapia

KW - Transcription

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