Gαq negatively regulates the Wnt-β-catenin pathway and dorsal embryonic Xenopus laevis development

Ximena Soto, Roberto Mayor, Marcela Torrejón, Martín Montecino, María Victoria Hinrichs, Juan Olate

Resultado de la investigación: Contribución a una revistaArtículo

7 Citas (Scopus)

Resumen

The non-canonical Wnt/Ca2+ signaling pathway has been implicated in the regulation of axis formation and gastrulation movements during early Xenopus laevis embryo development, by antagonizing the canonical Wnt/β-catenin dorsalizing pathway and specifying ventral cell fate. However, the molecular mechanisms involved in this antagonist crosstalk are not known. Since Gαq is the main regulator of Ca2+ signaling in vertebrates and from this perspective probably involved in the events elicited by the non-canonical Wnt/Ca2+ pathway, we decided to study the effect of wild-type Xenopus Gq (xGαq) in dorso-ventral axis embryo patterning. Overexpression of xGαq or its endogenous activation at the dorsal animal region of Xenopus embryo both induced a strong ventralized phenotype and inhibited the expression of dorsal-specific mesoderm markers goosecoid and chordin. Dorsal expression of an xGαq dominant-negative mutant reverted the xGαq-induced ventralized phenotype. Finally, we observed that the Wnt8-induced secondary axis formation is reverted by endogenous xGαq activation, indicating that it is negatively regulating the Wnt/β-catenin pathway.

Idioma originalInglés
Páginas (desde-hasta)483-490
Número de páginas8
PublicaciónJournal of Cellular Physiology
Volumen214
N.º2
DOI
EstadoPublicada - feb 2008

Áreas temáticas de ASJC Scopus

  • Biología celular
  • Bioquímica clínica
  • Fisiología

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