Gαq negatively regulates the Wnt-β-catenin pathway and dorsal embryonic Xenopus laevis development

Ximena Soto, Roberto Mayor, Marcela Torrejón, Martín Montecino, María Victoria Hinrichs, Juan Olate

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

The non-canonical Wnt/Ca2+ signaling pathway has been implicated in the regulation of axis formation and gastrulation movements during early Xenopus laevis embryo development, by antagonizing the canonical Wnt/β-catenin dorsalizing pathway and specifying ventral cell fate. However, the molecular mechanisms involved in this antagonist crosstalk are not known. Since Gαq is the main regulator of Ca2+ signaling in vertebrates and from this perspective probably involved in the events elicited by the non-canonical Wnt/Ca2+ pathway, we decided to study the effect of wild-type Xenopus Gq (xGαq) in dorso-ventral axis embryo patterning. Overexpression of xGαq or its endogenous activation at the dorsal animal region of Xenopus embryo both induced a strong ventralized phenotype and inhibited the expression of dorsal-specific mesoderm markers goosecoid and chordin. Dorsal expression of an xGαq dominant-negative mutant reverted the xGαq-induced ventralized phenotype. Finally, we observed that the Wnt8-induced secondary axis formation is reverted by endogenous xGαq activation, indicating that it is negatively regulating the Wnt/β-catenin pathway.

Idioma originalEnglish
Páginas (desde-hasta)483-490
Número de páginas8
PublicaciónJournal of Cellular Physiology
Volumen214
N.º2
DOI
EstadoPublished - feb 2008

Huella dactilar

Catenins
Wnt Signaling Pathway
Xenopus laevis
Xenopus
Chemical activation
Crosstalk
Animals
Embryonic Structures
Phenotype
Gastrulation
Mesoderm
Embryonic Development
Vertebrates
chordin

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Citar esto

Soto, Ximena ; Mayor, Roberto ; Torrejón, Marcela ; Montecino, Martín ; Hinrichs, María Victoria ; Olate, Juan. / Gαq negatively regulates the Wnt-β-catenin pathway and dorsal embryonic Xenopus laevis development. En: Journal of Cellular Physiology. 2008 ; Vol. 214, N.º 2. pp. 483-490.
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Gαq negatively regulates the Wnt-β-catenin pathway and dorsal embryonic Xenopus laevis development. / Soto, Ximena; Mayor, Roberto; Torrejón, Marcela; Montecino, Martín; Hinrichs, María Victoria; Olate, Juan.

En: Journal of Cellular Physiology, Vol. 214, N.º 2, 02.2008, p. 483-490.

Resultado de la investigación: Article

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