Functional analysis of the subunits of the chromatin assembly factor RSF

Alejandra Loyola, Jing Yi Huang, Gary LeRoy, Sherrie Hu, Yuh Hwa Wang, Robert J. Donnelly, William S. Lane, Sheng Chung Lee, Danny Reinberg

Resultado de la investigación: Contribución a una revistaArtículo

79 Citas (Scopus)

Resumen

The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.

Idioma originalInglés
Páginas (desde-hasta)6759-6768
Número de páginas10
PublicaciónMolecular and Cellular Biology
Volumen23
N.º19
DOI
EstadoPublicada - oct 2003

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Biología celular

Huella Profundice en los temas de investigación de 'Functional analysis of the subunits of the chromatin assembly factor RSF'. En conjunto forman una huella única.

  • Citar esto

    Loyola, A., Huang, J. Y., LeRoy, G., Hu, S., Wang, Y. H., Donnelly, R. J., Lane, W. S., Lee, S. C., & Reinberg, D. (2003). Functional analysis of the subunits of the chromatin assembly factor RSF. Molecular and Cellular Biology, 23(19), 6759-6768. https://doi.org/10.1128/MCB.23.19.6759-6768.2003