Functional analysis of the subunits of the chromatin assembly factor RSF

Alejandra Loyola, Jing Yi Huang, Gary LeRoy, Sherrie Hu, Yuh Hwa Wang, Robert J. Donnelly, William S. Lane, Sheng Chung Lee, Danny Reinberg

Resultado de la investigación: Article

78 Citas (Scopus)

Resumen

The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.

Idioma originalEnglish
Páginas (desde-hasta)6759-6768
Número de páginas10
PublicaciónMolecular and Cellular Biology
Volumen23
N.º19
DOI
EstadoPublished - oct 2003

Huella dactilar

Chromatin Assembly and Disassembly
Nucleosomes
Hepatitis B virus
Genes
Adenosine Triphosphatases
Dissection
Organism Cloning
Proteins
Complementary DNA
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Citar esto

Loyola, A., Huang, J. Y., LeRoy, G., Hu, S., Wang, Y. H., Donnelly, R. J., ... Reinberg, D. (2003). Functional analysis of the subunits of the chromatin assembly factor RSF. Molecular and Cellular Biology, 23(19), 6759-6768. https://doi.org/10.1128/MCB.23.19.6759-6768.2003
Loyola, Alejandra ; Huang, Jing Yi ; LeRoy, Gary ; Hu, Sherrie ; Wang, Yuh Hwa ; Donnelly, Robert J. ; Lane, William S. ; Lee, Sheng Chung ; Reinberg, Danny. / Functional analysis of the subunits of the chromatin assembly factor RSF. En: Molecular and Cellular Biology. 2003 ; Vol. 23, N.º 19. pp. 6759-6768.
@article{99e36e2f3286428d8ee3ed2ef7881ce7,
title = "Functional analysis of the subunits of the chromatin assembly factor RSF",
abstract = "The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.",
author = "Alejandra Loyola and Huang, {Jing Yi} and Gary LeRoy and Sherrie Hu and Wang, {Yuh Hwa} and Donnelly, {Robert J.} and Lane, {William S.} and Lee, {Sheng Chung} and Danny Reinberg",
year = "2003",
month = "10",
doi = "10.1128/MCB.23.19.6759-6768.2003",
language = "English",
volume = "23",
pages = "6759--6768",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "19",

}

Loyola, A, Huang, JY, LeRoy, G, Hu, S, Wang, YH, Donnelly, RJ, Lane, WS, Lee, SC & Reinberg, D 2003, 'Functional analysis of the subunits of the chromatin assembly factor RSF', Molecular and Cellular Biology, vol. 23, n.º 19, pp. 6759-6768. https://doi.org/10.1128/MCB.23.19.6759-6768.2003

Functional analysis of the subunits of the chromatin assembly factor RSF. / Loyola, Alejandra; Huang, Jing Yi; LeRoy, Gary; Hu, Sherrie; Wang, Yuh Hwa; Donnelly, Robert J.; Lane, William S.; Lee, Sheng Chung; Reinberg, Danny.

En: Molecular and Cellular Biology, Vol. 23, N.º 19, 10.2003, p. 6759-6768.

Resultado de la investigación: Article

TY - JOUR

T1 - Functional analysis of the subunits of the chromatin assembly factor RSF

AU - Loyola, Alejandra

AU - Huang, Jing Yi

AU - LeRoy, Gary

AU - Hu, Sherrie

AU - Wang, Yuh Hwa

AU - Donnelly, Robert J.

AU - Lane, William S.

AU - Lee, Sheng Chung

AU - Reinberg, Danny

PY - 2003/10

Y1 - 2003/10

N2 - The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.

AB - The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.

UR - http://www.scopus.com/inward/record.url?scp=0141781112&partnerID=8YFLogxK

U2 - 10.1128/MCB.23.19.6759-6768.2003

DO - 10.1128/MCB.23.19.6759-6768.2003

M3 - Article

C2 - 12972596

AN - SCOPUS:0141781112

VL - 23

SP - 6759

EP - 6768

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 19

ER -