First description of atypical furunculosis in freshwater farmed Atlantic salmon, Salmo salar L., in Chile

M. Godoy, V. Gherardelli, A. Heisinger, J. Fernández, P. Olmos, L. Ovalle, P. Ilardi, R. Avendaño-Herrera

Resultado de la investigación: Article

12 Citas (Scopus)

Resumen

We report the first isolation, identification and characterization of a group of Chilean strains of atypical Aeromonas salmonicida isolated from freshwater farmed Atlantic salmon, Salmo salar. Affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. Outbreaks of the disease occurred in two farms in the south of Chile about 2200 km apart. Five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging to Aeromonas salmonicida. Although the bacterial isolates were phenotypically homogeneous, minor differences with the reference strain A. salmonicida subsp. salmonicida ATCC 33658 were noted. Three specific primer sets and partial 16S rRNA gene sequencing allowed the identification of the Chilean isolates as atypical A. salmonicida, with A. salmonicida subsp. achromogenes and A. salmonicida subsp. masoucida as their closest relatives (100% sequence similarity). Molecular typing indicated that the atypical isolates belong to two genetic groups that were associated with the geographical origin.

Idioma originalEnglish
Páginas (desde-hasta)441-449
Número de páginas9
PublicaciónJournal of Fish Diseases
Volumen33
N.º5
DOI
EstadoPublished - 1 may 2010

Huella dactilar

Aeromonas salmonicida
Furunculosis
furunculosis
Salmo salar
Chile
Fresh Water
assay
farm
gene
fish
Aeromonas salmonicida subsp. achromogenes
Aeromonas salmonicida subsp. salmonicida
fluorescent antibody technique
hemorrhage
Molecular Typing
ribosomal RNA
rRNA Genes
farms
liver
Ulcer

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Citar esto

Godoy, M. ; Gherardelli, V. ; Heisinger, A. ; Fernández, J. ; Olmos, P. ; Ovalle, L. ; Ilardi, P. ; Avendaño-Herrera, R. / First description of atypical furunculosis in freshwater farmed Atlantic salmon, Salmo salar L., in Chile. En: Journal of Fish Diseases. 2010 ; Vol. 33, N.º 5. pp. 441-449.
@article{cede645e9dae468d9c95fb5c5c505f3a,
title = "First description of atypical furunculosis in freshwater farmed Atlantic salmon, Salmo salar L., in Chile",
abstract = "We report the first isolation, identification and characterization of a group of Chilean strains of atypical Aeromonas salmonicida isolated from freshwater farmed Atlantic salmon, Salmo salar. Affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. Outbreaks of the disease occurred in two farms in the south of Chile about 2200 km apart. Five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging to Aeromonas salmonicida. Although the bacterial isolates were phenotypically homogeneous, minor differences with the reference strain A. salmonicida subsp. salmonicida ATCC 33658 were noted. Three specific primer sets and partial 16S rRNA gene sequencing allowed the identification of the Chilean isolates as atypical A. salmonicida, with A. salmonicida subsp. achromogenes and A. salmonicida subsp. masoucida as their closest relatives (100{\%} sequence similarity). Molecular typing indicated that the atypical isolates belong to two genetic groups that were associated with the geographical origin.",
keywords = "Aeromonas salmonicida, Atlantic salmon, Atypical furunculosis, Chile, Fresh water",
author = "M. Godoy and V. Gherardelli and A. Heisinger and J. Fern{\'a}ndez and P. Olmos and L. Ovalle and P. Ilardi and R. Avenda{\~n}o-Herrera",
year = "2010",
month = "5",
day = "1",
doi = "10.1111/j.1365-2761.2010.01142.x",
language = "English",
volume = "33",
pages = "441--449",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
number = "5",

}

First description of atypical furunculosis in freshwater farmed Atlantic salmon, Salmo salar L., in Chile. / Godoy, M.; Gherardelli, V.; Heisinger, A.; Fernández, J.; Olmos, P.; Ovalle, L.; Ilardi, P.; Avendaño-Herrera, R.

En: Journal of Fish Diseases, Vol. 33, N.º 5, 01.05.2010, p. 441-449.

Resultado de la investigación: Article

TY - JOUR

T1 - First description of atypical furunculosis in freshwater farmed Atlantic salmon, Salmo salar L., in Chile

AU - Godoy, M.

AU - Gherardelli, V.

AU - Heisinger, A.

AU - Fernández, J.

AU - Olmos, P.

AU - Ovalle, L.

AU - Ilardi, P.

AU - Avendaño-Herrera, R.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - We report the first isolation, identification and characterization of a group of Chilean strains of atypical Aeromonas salmonicida isolated from freshwater farmed Atlantic salmon, Salmo salar. Affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. Outbreaks of the disease occurred in two farms in the south of Chile about 2200 km apart. Five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging to Aeromonas salmonicida. Although the bacterial isolates were phenotypically homogeneous, minor differences with the reference strain A. salmonicida subsp. salmonicida ATCC 33658 were noted. Three specific primer sets and partial 16S rRNA gene sequencing allowed the identification of the Chilean isolates as atypical A. salmonicida, with A. salmonicida subsp. achromogenes and A. salmonicida subsp. masoucida as their closest relatives (100% sequence similarity). Molecular typing indicated that the atypical isolates belong to two genetic groups that were associated with the geographical origin.

AB - We report the first isolation, identification and characterization of a group of Chilean strains of atypical Aeromonas salmonicida isolated from freshwater farmed Atlantic salmon, Salmo salar. Affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. Outbreaks of the disease occurred in two farms in the south of Chile about 2200 km apart. Five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging to Aeromonas salmonicida. Although the bacterial isolates were phenotypically homogeneous, minor differences with the reference strain A. salmonicida subsp. salmonicida ATCC 33658 were noted. Three specific primer sets and partial 16S rRNA gene sequencing allowed the identification of the Chilean isolates as atypical A. salmonicida, with A. salmonicida subsp. achromogenes and A. salmonicida subsp. masoucida as their closest relatives (100% sequence similarity). Molecular typing indicated that the atypical isolates belong to two genetic groups that were associated with the geographical origin.

KW - Aeromonas salmonicida

KW - Atlantic salmon

KW - Atypical furunculosis

KW - Chile

KW - Fresh water

UR - http://www.scopus.com/inward/record.url?scp=77952814483&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2761.2010.01142.x

DO - 10.1111/j.1365-2761.2010.01142.x

M3 - Article

C2 - 20298453

AN - SCOPUS:77952814483

VL - 33

SP - 441

EP - 449

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 5

ER -