Features of basal and race-specific defences in photosynthetic Arabidopsis thaliana suspension cultured cells

Short communication

Nicolás Miguel Cecchini, Mariela Inés Monteoliva, Francisca Blanco, Loreto Holuigue, María Elena Álvarez

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

Plant suspension cell cultures display many features of the innate immune responses observed in planta and have been extensively applied to the study of basal and race-specific defences. However, no single model including photosynthetic cultured cells has been used for the exhaustive characterization of both basal and race-specific defences to date. In this article, we report the activation of basal and race-specific defences in green cultured cells from Arabidopsis thaliana. Inoculation of cultured cells with isogenic virulent or avirulent strains of Pseudomonas syringae pv. tomato DC3000 (Pst) was used to evaluate race-specific defences. The proliferation of avirulent Pst was found to be lower than that of virulent Pst in the inoculated cultures. Extracellular pH changes, sustained oxidative burst (5-13 h post-inoculation), enhancement of salicylic acid, and massive cell death were specifically stimulated by the avirulent bacterium. Neither avirulent nor virulent Pst induced markers of basal resistance, such as callose deposition or early oxidative burst (1-5 h post-inoculation). However, both basal defences were activated when cells were exposed to Pseudomonas syringae pv. phaseolicola or to the Pst mutant defective in the type III secretion system (TTSS), Pst-hrpL-. Thus, in these cells, basal defences may be inhibited by Pst in a TTSS-dependent manner. Recapitulation of classical defence features demonstrates the usefulness of this system for the fine characterization of plant innate immune components.

Idioma originalEnglish
Páginas (desde-hasta)305-310
Número de páginas6
PublicaciónMolecular Plant Pathology
Volumen10
N.º2
DOI
EstadoPublished - mar 2009

Huella dactilar

Arabidopsis
Cell Communication
cultured cells
Cultured Cells
Suspensions
Arabidopsis thaliana
communication
Pseudomonas syringae
type III secretion system
Respiratory Burst
inoculation
Pseudomonas syringae pv. phaseolicola
Pseudomonas syringae pv. tomato
Salicylic Acid
avirulent strains
callose
secretion
Plant Cells
Lycopersicon esculentum
Plantae

ASJC Scopus subject areas

  • Molecular Biology
  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

Citar esto

Cecchini, Nicolás Miguel ; Monteoliva, Mariela Inés ; Blanco, Francisca ; Holuigue, Loreto ; Álvarez, María Elena. / Features of basal and race-specific defences in photosynthetic Arabidopsis thaliana suspension cultured cells : Short communication. En: Molecular Plant Pathology. 2009 ; Vol. 10, N.º 2. pp. 305-310.
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Features of basal and race-specific defences in photosynthetic Arabidopsis thaliana suspension cultured cells : Short communication. / Cecchini, Nicolás Miguel; Monteoliva, Mariela Inés; Blanco, Francisca; Holuigue, Loreto; Álvarez, María Elena.

En: Molecular Plant Pathology, Vol. 10, N.º 2, 03.2009, p. 305-310.

Resultado de la investigación: Article

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