Extended-spectrum β-lactamases belonging to CTX-M group produced by Escherichia coli strains isolated from companion animals treated with enrofloxacin

Andrea Moreno, Helia Bello, Drago Guggiana, Mariana Domínguez, Gerardo González

Resultado de la investigación: Article

42 Citas (Scopus)

Resumen

We studied the antibiotic resistance among Escherichia coli isolates obtained from fecal samples of dogs and cats treated and untreated with enrofloxacin in veterinary clinics. Resistant patterns of 70 strains and the presence of extended-spectrum β-lactamase (ESBL) were studied. The genes encoding the following families of β-lactamases: CTX-M, GES, PER, TEM and SHV, were investigated by PCR-RFLP and sequencing. The strains isolated from enrofloxacin-treated animals were multi-drug-resistant exhibiting resistant patterns including fluorquinolones, β-lactams, aminoglycosides, tetracycline and phenicols. On the contrary, the strains obtained from the untreated group of animals exhibited narrower antibiotic resistant profiles. The synthesis of ESBL was detected in 14 strains (20%) isolated from treated animals. The ESBL encoded by genes blaCTX-M-1, blaCTX-M-9 group and blaPER-2 were detected by PCR. We believe that this is the first report on the presence of ESBL in E. coli strains isolated from small animals in Chile, and the first report of β-lactamase belonging to the CTX-M-9 group (CTX-M-14). The presence of these genes in bacteria isolated from pets is an important fact that constitutes a public health concern.

Idioma originalEnglish
Páginas (desde-hasta)203-208
Número de páginas6
PublicaciónVeterinary Microbiology
Volumen129
N.º1-2
DOI
EstadoPublished - 25 may 2008

Huella dactilar

enrofloxacin
Pets
pets
Escherichia coli
Genes
animals
Animal Hospitals
Lactams
Polymerase Chain Reaction
Chile
lactams
Aminoglycosides
Microbial Drug Resistance
Tetracycline
aminoglycosides
Restriction Fragment Length Polymorphisms
genes
veterinary clinics
Cats
tetracycline

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Citar esto

Moreno, Andrea ; Bello, Helia ; Guggiana, Drago ; Domínguez, Mariana ; González, Gerardo. / Extended-spectrum β-lactamases belonging to CTX-M group produced by Escherichia coli strains isolated from companion animals treated with enrofloxacin. En: Veterinary Microbiology. 2008 ; Vol. 129, N.º 1-2. pp. 203-208.
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abstract = "We studied the antibiotic resistance among Escherichia coli isolates obtained from fecal samples of dogs and cats treated and untreated with enrofloxacin in veterinary clinics. Resistant patterns of 70 strains and the presence of extended-spectrum β-lactamase (ESBL) were studied. The genes encoding the following families of β-lactamases: CTX-M, GES, PER, TEM and SHV, were investigated by PCR-RFLP and sequencing. The strains isolated from enrofloxacin-treated animals were multi-drug-resistant exhibiting resistant patterns including fluorquinolones, β-lactams, aminoglycosides, tetracycline and phenicols. On the contrary, the strains obtained from the untreated group of animals exhibited narrower antibiotic resistant profiles. The synthesis of ESBL was detected in 14 strains (20{\%}) isolated from treated animals. The ESBL encoded by genes blaCTX-M-1, blaCTX-M-9 group and blaPER-2 were detected by PCR. We believe that this is the first report on the presence of ESBL in E. coli strains isolated from small animals in Chile, and the first report of β-lactamase belonging to the CTX-M-9 group (CTX-M-14). The presence of these genes in bacteria isolated from pets is an important fact that constitutes a public health concern.",
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Extended-spectrum β-lactamases belonging to CTX-M group produced by Escherichia coli strains isolated from companion animals treated with enrofloxacin. / Moreno, Andrea; Bello, Helia; Guggiana, Drago; Domínguez, Mariana; González, Gerardo.

En: Veterinary Microbiology, Vol. 129, N.º 1-2, 25.05.2008, p. 203-208.

Resultado de la investigación: Article

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T1 - Extended-spectrum β-lactamases belonging to CTX-M group produced by Escherichia coli strains isolated from companion animals treated with enrofloxacin

AU - Moreno, Andrea

AU - Bello, Helia

AU - Guggiana, Drago

AU - Domínguez, Mariana

AU - González, Gerardo

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AB - We studied the antibiotic resistance among Escherichia coli isolates obtained from fecal samples of dogs and cats treated and untreated with enrofloxacin in veterinary clinics. Resistant patterns of 70 strains and the presence of extended-spectrum β-lactamase (ESBL) were studied. The genes encoding the following families of β-lactamases: CTX-M, GES, PER, TEM and SHV, were investigated by PCR-RFLP and sequencing. The strains isolated from enrofloxacin-treated animals were multi-drug-resistant exhibiting resistant patterns including fluorquinolones, β-lactams, aminoglycosides, tetracycline and phenicols. On the contrary, the strains obtained from the untreated group of animals exhibited narrower antibiotic resistant profiles. The synthesis of ESBL was detected in 14 strains (20%) isolated from treated animals. The ESBL encoded by genes blaCTX-M-1, blaCTX-M-9 group and blaPER-2 were detected by PCR. We believe that this is the first report on the presence of ESBL in E. coli strains isolated from small animals in Chile, and the first report of β-lactamase belonging to the CTX-M-9 group (CTX-M-14). The presence of these genes in bacteria isolated from pets is an important fact that constitutes a public health concern.

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