Expression of three recombinant homodimeric isoforms of PDGF in saccharomyces cerevisiae: Evidence for difference in receptor binding and functional activities

Arne Östman, Gudrun Bäckström, Noel Fong, Christer Betsholtz, Christer Wernstedt, Ulf Hellman, Bengt Westermark, Pablo Valenzuela, Carl Henrik Heldin

Resultado de la investigación: Contribución a una revistaArtículo

73 Citas (Scopus)

Resumen

Three recombinant homodimeric isoforms of platelet-derived growth factor (PDGF) were produced and purified in milligram quantities by expression of PDGF A-and B-chains in yeast cells. Structural analysis of the purified short and long variants of PDGF-AA (PDGF-AAS and PDGF-AAL) and PDGF-BB showed that they had been properly processed and assembled into dimers. PDGF-AAs and PDGF-AAL were found to bind only to the PDGF A-type receptor on human fibroblasts, with affinities of 0.1 and 0.2 uM, respectively. PDGF-BB bound to cells with A-and B-type receptors and to cells with B-type receptor only with affinities of 0.6 nM in both cases. Each fibroblast appeared to express about 4-5 times more B-type receptors than A-type receptors. The maximal mitogenic response to PDGF-BB of human fibroblasts was almost 2-fold higher than that induced by either of the two PDGF-AA forms. The three isoforms of PDGF also stimulated growth in soft agar of human fibroblasts with PDGF-BB inducing a higher maximal response.

Idioma originalInglés
Páginas (desde-hasta)271-281
Número de páginas11
PublicaciónGrowth Factors
Volumen1
N.º3
DOI
EstadoPublicada - 1 ene 1989

Áreas temáticas de ASJC Scopus

  • Endocrinología
  • Bioquímica clínica
  • Biología celular

Huella Profundice en los temas de investigación de 'Expression of three recombinant homodimeric isoforms of PDGF in saccharomyces cerevisiae: Evidence for difference in receptor binding and functional activities'. En conjunto forman una huella única.

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    Östman, A., Bäckström, G., Fong, N., Betsholtz, C., Wernstedt, C., Hellman, U., Westermark, B., Valenzuela, P., & Heldin, C. H. (1989). Expression of three recombinant homodimeric isoforms of PDGF in saccharomyces cerevisiae: Evidence for difference in receptor binding and functional activities. Growth Factors, 1(3), 271-281. https://doi.org/10.3109/08977198908998003