Resumen
Three recombinant homodimeric isoforms of platelet-derived growth factor (PDGF) were produced and purified in milligram quantities by expression of PDGF A-and B-chains in yeast cells. Structural analysis of the purified short and long variants of PDGF-AA (PDGF-AAS and PDGF-AAL) and PDGF-BB showed that they had been properly processed and assembled into dimers. PDGF-AAs and PDGF-AAL were found to bind only to the PDGF A-type receptor on human fibroblasts, with affinities of 0.1 and 0.2 uM, respectively. PDGF-BB bound to cells with A-and B-type receptors and to cells with B-type receptor only with affinities of 0.6 nM in both cases. Each fibroblast appeared to express about 4-5 times more B-type receptors than A-type receptors. The maximal mitogenic response to PDGF-BB of human fibroblasts was almost 2-fold higher than that induced by either of the two PDGF-AA forms. The three isoforms of PDGF also stimulated growth in soft agar of human fibroblasts with PDGF-BB inducing a higher maximal response.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 271-281 |
| Número de páginas | 11 |
| Publicación | Growth Factors |
| Volumen | 1 |
| N.º | 3 |
| DOI | |
| Estado | Published - 1 ene 1989 |
Huella dactilar
ASJC Scopus subject areas
- Endocrinology
- Clinical Biochemistry
- Cell Biology
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Expression of three recombinant homodimeric isoforms of PDGF in saccharomyces cerevisiae : Evidence for difference in receptor binding and functional activities. / Östman, Arne; Bäckström, Gudrun; Fong, Noel; Betsholtz, Christer; Wernstedt, Christer; Hellman, Ulf; Westermark, Bengt; Valenzuela, Pablo; Heldin, Carl Henrik.
En: Growth Factors, Vol. 1, N.º 3, 01.01.1989, p. 271-281.Resultado de la investigación: Article
TY - JOUR
T1 - Expression of three recombinant homodimeric isoforms of PDGF in saccharomyces cerevisiae
T2 - Evidence for difference in receptor binding and functional activities
AU - Östman, Arne
AU - Bäckström, Gudrun
AU - Fong, Noel
AU - Betsholtz, Christer
AU - Wernstedt, Christer
AU - Hellman, Ulf
AU - Westermark, Bengt
AU - Valenzuela, Pablo
AU - Heldin, Carl Henrik
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Three recombinant homodimeric isoforms of platelet-derived growth factor (PDGF) were produced and purified in milligram quantities by expression of PDGF A-and B-chains in yeast cells. Structural analysis of the purified short and long variants of PDGF-AA (PDGF-AAS and PDGF-AAL) and PDGF-BB showed that they had been properly processed and assembled into dimers. PDGF-AAs and PDGF-AAL were found to bind only to the PDGF A-type receptor on human fibroblasts, with affinities of 0.1 and 0.2 uM, respectively. PDGF-BB bound to cells with A-and B-type receptors and to cells with B-type receptor only with affinities of 0.6 nM in both cases. Each fibroblast appeared to express about 4-5 times more B-type receptors than A-type receptors. The maximal mitogenic response to PDGF-BB of human fibroblasts was almost 2-fold higher than that induced by either of the two PDGF-AA forms. The three isoforms of PDGF also stimulated growth in soft agar of human fibroblasts with PDGF-BB inducing a higher maximal response.
AB - Three recombinant homodimeric isoforms of platelet-derived growth factor (PDGF) were produced and purified in milligram quantities by expression of PDGF A-and B-chains in yeast cells. Structural analysis of the purified short and long variants of PDGF-AA (PDGF-AAS and PDGF-AAL) and PDGF-BB showed that they had been properly processed and assembled into dimers. PDGF-AAs and PDGF-AAL were found to bind only to the PDGF A-type receptor on human fibroblasts, with affinities of 0.1 and 0.2 uM, respectively. PDGF-BB bound to cells with A-and B-type receptors and to cells with B-type receptor only with affinities of 0.6 nM in both cases. Each fibroblast appeared to express about 4-5 times more B-type receptors than A-type receptors. The maximal mitogenic response to PDGF-BB of human fibroblasts was almost 2-fold higher than that induced by either of the two PDGF-AA forms. The three isoforms of PDGF also stimulated growth in soft agar of human fibroblasts with PDGF-BB inducing a higher maximal response.
KW - Isoforms
KW - PDGF
KW - Yeast expression
UR - http://www.scopus.com/inward/record.url?scp=0024339109&partnerID=8YFLogxK
U2 - 10.3109/08977198908998003
DO - 10.3109/08977198908998003
M3 - Article
VL - 1
SP - 271
EP - 281
JO - Growth Factors
JF - Growth Factors
SN - 0897-7194
IS - 3
ER -