Expression of the yggE gene protects Escherichia coli from potassium tellurite-generated oxidative stress

Lillian G. Acuña, Iván L. Calderón, Alex O. Elías, Miguel E. Castro, Claudio C. Vásquez

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

Potassium tellurite is highly toxic to most forms of life and specific bacterial tellurite defense mechanisms are not fully understood to date. Recent evidence suggests that tellurite would exert its toxic effects, at least in part, through the generation of superoxide anion that occurs concomitantly with intracellular tellurite (Te4+) reduction to elemental tellurium (Teo). In this work the putative antioxidant role of YggE from Escherichia coli, a highly conserved protein in several bacterial species and whose function is still a matter of speculation, was studied. When exposed to tellurite, E. coli lacking yggE exhibited increased activity of superoxide dismutase and fumarase C, augmented levels of reactive oxygen species and high concentration of carbonyl groups in proteins. Upon genetic complementation with the homologous yggE gene these values were restored to those observed in the parental, isogenic, wild type strain, suggesting a direct participation of YggE in E. coli tolerance to tellurite.

Idioma originalEnglish
Páginas (desde-hasta)473-476
Número de páginas4
PublicaciónArchives of Microbiology
Volumen191
N.º5
DOI
EstadoPublished - 1 may 2009

Huella dactilar

Oxidative stress
Escherichia coli
Oxidative Stress
Genes
Gene Expression
Poisons
Tellurium
Superoxides
Superoxide Dismutase
Reactive Oxygen Species
Proteins
Antioxidants
potassium tellurate(IV)
tellurous acid

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Citar esto

Acuña, Lillian G. ; Calderón, Iván L. ; Elías, Alex O. ; Castro, Miguel E. ; Vásquez, Claudio C. / Expression of the yggE gene protects Escherichia coli from potassium tellurite-generated oxidative stress. En: Archives of Microbiology. 2009 ; Vol. 191, N.º 5. pp. 473-476.
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Expression of the yggE gene protects Escherichia coli from potassium tellurite-generated oxidative stress. / Acuña, Lillian G.; Calderón, Iván L.; Elías, Alex O.; Castro, Miguel E.; Vásquez, Claudio C.

En: Archives of Microbiology, Vol. 191, N.º 5, 01.05.2009, p. 473-476.

Resultado de la investigación: Article

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AU - Vásquez, Claudio C.

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