Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes

Claudio Villota, América Campos, Soledad Vidaurre, Luciana Oliveira-Cruz, Enrique Boccardo, Verónica A. Burzio, Manuel Varas, Jaime Villegas, Luisa L. Villa, Pablo D T Valenzuela, Miguel Socías, Sally Roberts, Luis O. Burzio

Resultado de la investigación: Article

22 Citas (Scopus)

Resumen

The study of RNA and DNA oncogenic viruses has proved invaluable in the discovery of key cellular pathways that are rendered dysfunctional during cancer progression. An example is high risk human papillomavirus (HPV), the etiological agent of cervical cancer. The role of HPV oncogenes in cellular immortalization and transformation has been extensively investigated. We reported the differential expression of a family of human mitochondrial non-coding RNAs (ncRNAs) between normal and cancer cells. Normal cells express a sense mitochondrial ncRNA (SncmtRNA) that seems to be required for cell proliferation and two antisense transcripts (ASncmtRNAs). In contrast, the ASncmtRNAs are down-regulated in cancer cells. To shed some light on the mechanisms that trigger down-regulation of the ASncmtRNAs, we studied human keratinocytes (HFK) immortalized with HPV. Here we show that immortalization of HFK with HPV-16 or 18 causes down-regulation of the ASncmtRNAs and induces the expression of a new sense transcript named SncmtRNA-2. Transduction of HFK with both E6 and E7 is sufficient to induce expression of SncmtRNA-2. Moreover, E2 oncogene is involved in down-regulation of the ASncmtRNAs. Knockdown of E2 in immortalized cells reestablishes in a reversible manner the expression of the ASncmtRNAs, suggesting that endogenous cellular factors(s) could play functions analogous to E2 during non-HPV-induced oncogenesis.

Idioma originalEnglish
Páginas (desde-hasta)21303-21315
Número de páginas13
PublicaciónJournal of Biological Chemistry
Volumen287
N.º25
DOI
EstadoPublished - 15 jun 2012

Huella dactilar

Untranslated RNA
Oncogenes
Oncogenic viruses
Cells
Cell proliferation
Down-Regulation
RNA
DNA
Human papillomavirus 18
Neoplasms
Oncogenic Viruses
DNA Viruses
Human papillomavirus 16
Keratinocytes
Uterine Cervical Neoplasms
Carcinogenesis
Cell Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Citar esto

Villota, Claudio ; Campos, América ; Vidaurre, Soledad ; Oliveira-Cruz, Luciana ; Boccardo, Enrique ; Burzio, Verónica A. ; Varas, Manuel ; Villegas, Jaime ; Villa, Luisa L. ; Valenzuela, Pablo D T ; Socías, Miguel ; Roberts, Sally ; Burzio, Luis O. / Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes. En: Journal of Biological Chemistry. 2012 ; Vol. 287, N.º 25. pp. 21303-21315.
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title = "Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes",
abstract = "The study of RNA and DNA oncogenic viruses has proved invaluable in the discovery of key cellular pathways that are rendered dysfunctional during cancer progression. An example is high risk human papillomavirus (HPV), the etiological agent of cervical cancer. The role of HPV oncogenes in cellular immortalization and transformation has been extensively investigated. We reported the differential expression of a family of human mitochondrial non-coding RNAs (ncRNAs) between normal and cancer cells. Normal cells express a sense mitochondrial ncRNA (SncmtRNA) that seems to be required for cell proliferation and two antisense transcripts (ASncmtRNAs). In contrast, the ASncmtRNAs are down-regulated in cancer cells. To shed some light on the mechanisms that trigger down-regulation of the ASncmtRNAs, we studied human keratinocytes (HFK) immortalized with HPV. Here we show that immortalization of HFK with HPV-16 or 18 causes down-regulation of the ASncmtRNAs and induces the expression of a new sense transcript named SncmtRNA-2. Transduction of HFK with both E6 and E7 is sufficient to induce expression of SncmtRNA-2. Moreover, E2 oncogene is involved in down-regulation of the ASncmtRNAs. Knockdown of E2 in immortalized cells reestablishes in a reversible manner the expression of the ASncmtRNAs, suggesting that endogenous cellular factors(s) could play functions analogous to E2 during non-HPV-induced oncogenesis.",
author = "Claudio Villota and Am{\'e}rica Campos and Soledad Vidaurre and Luciana Oliveira-Cruz and Enrique Boccardo and Burzio, {Ver{\'o}nica A.} and Manuel Varas and Jaime Villegas and Villa, {Luisa L.} and Valenzuela, {Pablo D T} and Miguel Soc{\'i}as and Sally Roberts and Burzio, {Luis O.}",
year = "2012",
month = "6",
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doi = "10.1074/jbc.M111.326694",
language = "English",
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Villota, C, Campos, A, Vidaurre, S, Oliveira-Cruz, L, Boccardo, E, Burzio, VA, Varas, M, Villegas, J, Villa, LL, Valenzuela, PDT, Socías, M, Roberts, S & Burzio, LO 2012, 'Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes', Journal of Biological Chemistry, vol. 287, n.º 25, pp. 21303-21315. https://doi.org/10.1074/jbc.M111.326694

Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes. / Villota, Claudio; Campos, América; Vidaurre, Soledad; Oliveira-Cruz, Luciana; Boccardo, Enrique; Burzio, Verónica A.; Varas, Manuel; Villegas, Jaime; Villa, Luisa L.; Valenzuela, Pablo D T; Socías, Miguel; Roberts, Sally; Burzio, Luis O.

En: Journal of Biological Chemistry, Vol. 287, N.º 25, 15.06.2012, p. 21303-21315.

Resultado de la investigación: Article

TY - JOUR

T1 - Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes

AU - Villota, Claudio

AU - Campos, América

AU - Vidaurre, Soledad

AU - Oliveira-Cruz, Luciana

AU - Boccardo, Enrique

AU - Burzio, Verónica A.

AU - Varas, Manuel

AU - Villegas, Jaime

AU - Villa, Luisa L.

AU - Valenzuela, Pablo D T

AU - Socías, Miguel

AU - Roberts, Sally

AU - Burzio, Luis O.

PY - 2012/6/15

Y1 - 2012/6/15

N2 - The study of RNA and DNA oncogenic viruses has proved invaluable in the discovery of key cellular pathways that are rendered dysfunctional during cancer progression. An example is high risk human papillomavirus (HPV), the etiological agent of cervical cancer. The role of HPV oncogenes in cellular immortalization and transformation has been extensively investigated. We reported the differential expression of a family of human mitochondrial non-coding RNAs (ncRNAs) between normal and cancer cells. Normal cells express a sense mitochondrial ncRNA (SncmtRNA) that seems to be required for cell proliferation and two antisense transcripts (ASncmtRNAs). In contrast, the ASncmtRNAs are down-regulated in cancer cells. To shed some light on the mechanisms that trigger down-regulation of the ASncmtRNAs, we studied human keratinocytes (HFK) immortalized with HPV. Here we show that immortalization of HFK with HPV-16 or 18 causes down-regulation of the ASncmtRNAs and induces the expression of a new sense transcript named SncmtRNA-2. Transduction of HFK with both E6 and E7 is sufficient to induce expression of SncmtRNA-2. Moreover, E2 oncogene is involved in down-regulation of the ASncmtRNAs. Knockdown of E2 in immortalized cells reestablishes in a reversible manner the expression of the ASncmtRNAs, suggesting that endogenous cellular factors(s) could play functions analogous to E2 during non-HPV-induced oncogenesis.

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U2 - 10.1074/jbc.M111.326694

DO - 10.1074/jbc.M111.326694

M3 - Article

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JO - Journal of Biological Chemistry

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