TY - JOUR
T1 - Expression of genes encoding laccase and manganese-dependent peroxidase in the fungus Ceriporiopsis subvermispora is mediated by an ACE1-like copper-fist transcription factor
AU - ALVAREZ HERRERA, JOSE MIGUEL
AU - Canessa, Paulo
AU - Mancilla, Rodrigo A.
AU - Polanco, Rubén
AU - Santibáñez, Paulina A.
AU - Vicuña, Rafael
N1 - Funding Information:
This work was financed by the Millenium Institute for Fundamental and Applied Biology and by Grant 1070588 from FONDECYT-Chile. P. Canessa is a predoctoral fellow supported by CONICYT-Chile.
PY - 2009/1
Y1 - 2009/1
N2 - The effect of copper on the expression of genes encoding the ligninolytic enzymes laccase (lcs) and manganese peroxidase (mnp) in Ceriporiopsis subvermispora was evaluated. This metal increased transcript levels of lcs, mnp1 and mnp2. This finding was not unexpected in the case of lcs, since its promoter contains a putative ACE element. Originally characterized in the yeast Saccharomyces cerevisiae, ACE is the target sequence of the ACE1 copper-responsive transcription factor in this microorganism. Analysis of the promoter regions of mnp genes revealed the presence of formerly unnoticed ACE elements. Based on the ace1 gene from Phanerochaete chrysosporium, we isolated and characterized an ACE1-like transcription factor from C. subvermispora (Cs-ACE1) through complementation of a S. cerevisiae ace1Δ strain. Surprisingly, ACE1 factors from both basidiomycetes exhibit substantial differences, not only structurally but also in their ability to complement the aforementioned yeast strain. Specific binding of Cs-ACE1 to its cognate DNA sequence was confirmed by electrophoretic mobility-shift assays.
AB - The effect of copper on the expression of genes encoding the ligninolytic enzymes laccase (lcs) and manganese peroxidase (mnp) in Ceriporiopsis subvermispora was evaluated. This metal increased transcript levels of lcs, mnp1 and mnp2. This finding was not unexpected in the case of lcs, since its promoter contains a putative ACE element. Originally characterized in the yeast Saccharomyces cerevisiae, ACE is the target sequence of the ACE1 copper-responsive transcription factor in this microorganism. Analysis of the promoter regions of mnp genes revealed the presence of formerly unnoticed ACE elements. Based on the ace1 gene from Phanerochaete chrysosporium, we isolated and characterized an ACE1-like transcription factor from C. subvermispora (Cs-ACE1) through complementation of a S. cerevisiae ace1Δ strain. Surprisingly, ACE1 factors from both basidiomycetes exhibit substantial differences, not only structurally but also in their ability to complement the aforementioned yeast strain. Specific binding of Cs-ACE1 to its cognate DNA sequence was confirmed by electrophoretic mobility-shift assays.
KW - ACE
KW - Ceriporiopsis subvermispora
KW - Copper
KW - Electrophoretic mobility-shift assays
KW - Laccase
KW - Ligninolytic
KW - Manganese peroxidase
KW - Multicopper oxidase
KW - Transcription factor
KW - copper-fist
UR - http://www.scopus.com/inward/record.url?scp=57649156230&partnerID=8YFLogxK
U2 - 10.1016/j.fgb.2008.10.002
DO - 10.1016/j.fgb.2008.10.002
M3 - Article
C2 - 18955150
AN - SCOPUS:57649156230
SN - 1087-1845
VL - 46
SP - 104
EP - 111
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
IS - 1
ER -