Expression of a novel non-coding mitochondrial RNA in human proliferating cells

Jaime Villegas, Veronica Burzio, Claudio Villota, Eduardo Landerer, Ronny Martinez, Marcela Santander, Rodrigo Martinez, Rodrigo Pinto, María I. Vera, Enrique Boccardo, Luisa L. Villa, Luis O. Burzio

Resultado de la investigación: Article

40 Citas (Scopus)

Resumen

Previously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5′ end of the mitochondrial 16S RNA (16S mtrRNA). Here, we report the structure of an equivalent transcript of 2374 nt which is over-expressed in human proliferating cells but not in resting cells. The transcript contains a hairpin structure comprising an IR of 815 nt linked to the 5′ end of the 16S mtrRNA and forming a long double-stranded structure or stem and a loop of 40 nt. The stem is resistant to RNase A and can be detected and isolated after digestion with the enzyme. This novel transcript is a non-coding RNA (ncRNA) and several evidences suggest that the transcript is synthesized in mitochondria. The expression of this transcript can be induced in resting lymphocytes stimulated with phytohaemagglutinin (PHA). Moreover, aphidicolin treatment of DU145 cells reversibly blocks proliferation and expression of the transcript. If the drug is removed, the cells re-assume proliferation and over-express the ncmtRNA. These results suggest that the expression of the ncmtRNA correlates with the replicative state of the cell and it may play a role in cell proliferation.

Idioma originalEnglish
Páginas (desde-hasta)7336-7347
Número de páginas12
PublicaciónNucleic Acids Research
Volumen35
N.º21
DOI
EstadoPublished - 1 dic 2007

Huella dactilar

Untranslated RNA
Nucleotides
Aphidicolin
Pancreatic Ribonuclease
Phytohemagglutinins
mitochondrial RNA
Digestion
Mitochondria
Cell Proliferation
Lymphocytes
Enzymes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Genetics

Citar esto

Villegas, J., Burzio, V., Villota, C., Landerer, E., Martinez, R., Santander, M., ... Burzio, L. O. (2007). Expression of a novel non-coding mitochondrial RNA in human proliferating cells. Nucleic Acids Research, 35(21), 7336-7347. https://doi.org/10.1093/nar/gkm863
Villegas, Jaime ; Burzio, Veronica ; Villota, Claudio ; Landerer, Eduardo ; Martinez, Ronny ; Santander, Marcela ; Martinez, Rodrigo ; Pinto, Rodrigo ; Vera, María I. ; Boccardo, Enrique ; Villa, Luisa L. ; Burzio, Luis O. / Expression of a novel non-coding mitochondrial RNA in human proliferating cells. En: Nucleic Acids Research. 2007 ; Vol. 35, N.º 21. pp. 7336-7347.
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abstract = "Previously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5′ end of the mitochondrial 16S RNA (16S mtrRNA). Here, we report the structure of an equivalent transcript of 2374 nt which is over-expressed in human proliferating cells but not in resting cells. The transcript contains a hairpin structure comprising an IR of 815 nt linked to the 5′ end of the 16S mtrRNA and forming a long double-stranded structure or stem and a loop of 40 nt. The stem is resistant to RNase A and can be detected and isolated after digestion with the enzyme. This novel transcript is a non-coding RNA (ncRNA) and several evidences suggest that the transcript is synthesized in mitochondria. The expression of this transcript can be induced in resting lymphocytes stimulated with phytohaemagglutinin (PHA). Moreover, aphidicolin treatment of DU145 cells reversibly blocks proliferation and expression of the transcript. If the drug is removed, the cells re-assume proliferation and over-express the ncmtRNA. These results suggest that the expression of the ncmtRNA correlates with the replicative state of the cell and it may play a role in cell proliferation.",
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Villegas, J, Burzio, V, Villota, C, Landerer, E, Martinez, R, Santander, M, Martinez, R, Pinto, R, Vera, MI, Boccardo, E, Villa, LL & Burzio, LO 2007, 'Expression of a novel non-coding mitochondrial RNA in human proliferating cells', Nucleic Acids Research, vol. 35, n.º 21, pp. 7336-7347. https://doi.org/10.1093/nar/gkm863

Expression of a novel non-coding mitochondrial RNA in human proliferating cells. / Villegas, Jaime; Burzio, Veronica; Villota, Claudio; Landerer, Eduardo; Martinez, Ronny; Santander, Marcela; Martinez, Rodrigo; Pinto, Rodrigo; Vera, María I.; Boccardo, Enrique; Villa, Luisa L.; Burzio, Luis O.

En: Nucleic Acids Research, Vol. 35, N.º 21, 01.12.2007, p. 7336-7347.

Resultado de la investigación: Article

TY - JOUR

T1 - Expression of a novel non-coding mitochondrial RNA in human proliferating cells

AU - Villegas, Jaime

AU - Burzio, Veronica

AU - Villota, Claudio

AU - Landerer, Eduardo

AU - Martinez, Ronny

AU - Santander, Marcela

AU - Martinez, Rodrigo

AU - Pinto, Rodrigo

AU - Vera, María I.

AU - Boccardo, Enrique

AU - Villa, Luisa L.

AU - Burzio, Luis O.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Previously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5′ end of the mitochondrial 16S RNA (16S mtrRNA). Here, we report the structure of an equivalent transcript of 2374 nt which is over-expressed in human proliferating cells but not in resting cells. The transcript contains a hairpin structure comprising an IR of 815 nt linked to the 5′ end of the 16S mtrRNA and forming a long double-stranded structure or stem and a loop of 40 nt. The stem is resistant to RNase A and can be detected and isolated after digestion with the enzyme. This novel transcript is a non-coding RNA (ncRNA) and several evidences suggest that the transcript is synthesized in mitochondria. The expression of this transcript can be induced in resting lymphocytes stimulated with phytohaemagglutinin (PHA). Moreover, aphidicolin treatment of DU145 cells reversibly blocks proliferation and expression of the transcript. If the drug is removed, the cells re-assume proliferation and over-express the ncmtRNA. These results suggest that the expression of the ncmtRNA correlates with the replicative state of the cell and it may play a role in cell proliferation.

AB - Previously, we reported the presence in mouse cells of a mitochondrial RNA which contains an inverted repeat (IR) of 121 nucleotides (nt) covalently linked to the 5′ end of the mitochondrial 16S RNA (16S mtrRNA). Here, we report the structure of an equivalent transcript of 2374 nt which is over-expressed in human proliferating cells but not in resting cells. The transcript contains a hairpin structure comprising an IR of 815 nt linked to the 5′ end of the 16S mtrRNA and forming a long double-stranded structure or stem and a loop of 40 nt. The stem is resistant to RNase A and can be detected and isolated after digestion with the enzyme. This novel transcript is a non-coding RNA (ncRNA) and several evidences suggest that the transcript is synthesized in mitochondria. The expression of this transcript can be induced in resting lymphocytes stimulated with phytohaemagglutinin (PHA). Moreover, aphidicolin treatment of DU145 cells reversibly blocks proliferation and expression of the transcript. If the drug is removed, the cells re-assume proliferation and over-express the ncmtRNA. These results suggest that the expression of the ncmtRNA correlates with the replicative state of the cell and it may play a role in cell proliferation.

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U2 - 10.1093/nar/gkm863

DO - 10.1093/nar/gkm863

M3 - Article

VL - 35

SP - 7336

EP - 7347

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 21

ER -