Expression and splice variant analysis of the zebrafish tcf4 transcription factor

Rodrigo M. Young, Ariel E. Reyes, Miguel L. Allende

Resultado de la investigación: Article

35 Citas (Scopus)

Resumen

Wnt signalling has been implicated in antero-posterior patterning of the vertebrate embryonic body axis and in a number of other developmental processes. One of the downstream effectors of Wnt signalling is the β-catenin protein which complexes with members of the Lef/tcf transcription factor family. In the zebrafish, specification of the head has been shown to be dependent on the Tcf3 protein which acts as a repressor of the posteriorizing activity of Wnt (Nature 407 (2000) 913). Here, we report the cloning and expression pattern of the zebrafish tcf4 gene. In embryos, we find that the tcf4 gene is highly regulated at the level of RNA splicing such that the variant proteins that are produced contain or lack domains proposed to be essential in repression or activation of transcription. Expression of tcf4 mRNA is first detected in a graded fashion in the anterior brain and subsequently becomes restricted to the dorsal diencephalon and anterior midbrain. There is also transient expression in the anterior rhombomeres of the hindbrain and in the developing gut.

Idioma originalEnglish
Páginas (desde-hasta)269-273
Número de páginas5
PublicaciónMechanisms of Development
Volumen117
N.º1-2
DOI
EstadoPublished - 1 sep 2002

Huella dactilar

Zebrafish
Transcription Factors
RNA Splicing
Catenins
Diencephalon
Rhombencephalon
Mesencephalon
Transcriptional Activation
Genes
Vertebrates
Organism Cloning
Proteins
Embryonic Structures
Head
Messenger RNA
Brain

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Citar esto

Young, Rodrigo M. ; Reyes, Ariel E. ; Allende, Miguel L. / Expression and splice variant analysis of the zebrafish tcf4 transcription factor. En: Mechanisms of Development. 2002 ; Vol. 117, N.º 1-2. pp. 269-273.
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Expression and splice variant analysis of the zebrafish tcf4 transcription factor. / Young, Rodrigo M.; Reyes, Ariel E.; Allende, Miguel L.

En: Mechanisms of Development, Vol. 117, N.º 1-2, 01.09.2002, p. 269-273.

Resultado de la investigación: Article

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