Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural

Loretto Contreras-Porcia, Camilo López-Cristoffanini, Carlos Lovazzano, María Rosa Flores-Molina, Daniela Thomas, Alejandra Núñez, Camila Fierro, Eduardo Guajardo, Juan A. Correa, Michael Kube, Richard Reinhardt

Resultado de la investigación: Article

17 Citas (Scopus)

Resumen

In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.

Idioma originalSpanish
Páginas (desde-hasta)933-958
Número de páginas26
PublicaciónLatin American Journal of Aquatic Research
Volumen41
N.º5
DOI
EstadoPublished - 1 ene 2013

Huella dactilar

Bangiales
desiccation (plant physiology)
desiccation
hydration
Rhodophyta
gene expression
algae
tolerance
protein
transcriptome
cytochrome
biochemical pathways
alga
NADH dehydrogenase
rocky shore
littoral zone
heat shock
fronds
heat shock proteins
cytochrome P-450

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science

Citar esto

Contreras-Porcia, Loretto ; López-Cristoffanini, Camilo ; Lovazzano, Carlos ; Flores-Molina, María Rosa ; Thomas, Daniela ; Núñez, Alejandra ; Fierro, Camila ; Guajardo, Eduardo ; Correa, Juan A. ; Kube, Michael ; Reinhardt, Richard. / Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural. En: Latin American Journal of Aquatic Research. 2013 ; Vol. 41, N.º 5. pp. 933-958.
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title = "Expresi{\'o}n diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidrataci{\'o}n y desecaci{\'o}n natural",
abstract = "In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15{\%} appears involved in protein synthesis, processing and degradation, 14.4{\%} are related to photosynthesis and chloroplast, 13.1{\%} to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6{\%} to cell wall metabolism, and 7.5{\%} are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.",
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Contreras-Porcia, L, López-Cristoffanini, C, Lovazzano, C, Flores-Molina, MR, Thomas, D, Núñez, A, Fierro, C, Guajardo, E, Correa, JA, Kube, M & Reinhardt, R 2013, 'Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural', Latin American Journal of Aquatic Research, vol. 41, n.º 5, pp. 933-958. https://doi.org/10.3856/vol41-issue5-fulltext-13

Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural. / Contreras-Porcia, Loretto; López-Cristoffanini, Camilo; Lovazzano, Carlos; Flores-Molina, María Rosa; Thomas, Daniela; Núñez, Alejandra; Fierro, Camila; Guajardo, Eduardo; Correa, Juan A.; Kube, Michael; Reinhardt, Richard.

En: Latin American Journal of Aquatic Research, Vol. 41, N.º 5, 01.01.2013, p. 933-958.

Resultado de la investigación: Article

TY - JOUR

T1 - Expresión diferencial de genes en Pyropia columbina (Bangiales, Rhodophyta) bajo hidratación y desecación natural

AU - Contreras-Porcia, Loretto

AU - López-Cristoffanini, Camilo

AU - Lovazzano, Carlos

AU - Flores-Molina, María Rosa

AU - Thomas, Daniela

AU - Núñez, Alejandra

AU - Fierro, Camila

AU - Guajardo, Eduardo

AU - Correa, Juan A.

AU - Kube, Michael

AU - Reinhardt, Richard

PY - 2013/1/1

Y1 - 2013/1/1

N2 - In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.

AB - In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.

KW - Desiccation stress

KW - ESTs

KW - Proteins

KW - Pyropia

KW - Seaweeds

KW - Transcriptomics

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JF - Latin American Journal of Aquatic Research

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