Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers

Nicolas Pacheco, Matias Orellana-Saez, Marzena Pepczynska, Javier Enrione, Monica Bassas-Galia, Jose M. Borrero-de Acuña, Flavia C. Zacconi, Andrés E. Marcoleta, Ignacio Poblete-Castro

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.

Idioma originalEnglish
PublicaciónJournal of Industrial Microbiology and Biotechnology
DOI
EstadoPublished - 1 ene 2019

Huella dactilar

Biopolymers
Pseudomonas
Glycerol
Phenotype
Batch Cell Culture Techniques
Temperature
Bioreactors
Bioconversion
Chain length
Temperature control
Microorganisms
Bacteria
Costs and Cost Analysis
Growth
Substrates
Costs

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Citar esto

Pacheco, Nicolas ; Orellana-Saez, Matias ; Pepczynska, Marzena ; Enrione, Javier ; Bassas-Galia, Monica ; Borrero-de Acuña, Jose M. ; Zacconi, Flavia C. ; Marcoleta, Andrés E. ; Poblete-Castro, Ignacio. / Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains : from unique phenotypes to novel biopolymers. En: Journal of Industrial Microbiology and Biotechnology. 2019.
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abstract = "Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3{\%} and 34.5{\%} of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.",
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author = "Nicolas Pacheco and Matias Orellana-Saez and Marzena Pepczynska and Javier Enrione and Monica Bassas-Galia and {Borrero-de Acu{\~n}a}, {Jose M.} and Zacconi, {Flavia C.} and Marcoleta, {Andr{\'e}s E.} and Ignacio Poblete-Castro",
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Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains : from unique phenotypes to novel biopolymers. / Pacheco, Nicolas; Orellana-Saez, Matias; Pepczynska, Marzena; Enrione, Javier; Bassas-Galia, Monica; Borrero-de Acuña, Jose M.; Zacconi, Flavia C.; Marcoleta, Andrés E.; Poblete-Castro, Ignacio.

En: Journal of Industrial Microbiology and Biotechnology, 01.01.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains

T2 - from unique phenotypes to novel biopolymers

AU - Pacheco, Nicolas

AU - Orellana-Saez, Matias

AU - Pepczynska, Marzena

AU - Enrione, Javier

AU - Bassas-Galia, Monica

AU - Borrero-de Acuña, Jose M.

AU - Zacconi, Flavia C.

AU - Marcoleta, Andrés E.

AU - Poblete-Castro, Ignacio

PY - 2019/1/1

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N2 - Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.

AB - Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.

KW - Antarctic Pseudomonas

KW - Exopolysaccharide

KW - Glycerol

KW - Low temperature

KW - Poly(3-hydroxyalkanoates)

KW - Psychrophiles

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DO - 10.1007/s10295-019-02186-2

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JF - Journal of Industrial Microbiology and Biotechnology

SN - 1367-5435

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