Excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells

Tania S. Quiroz, Pamela A. Nieto, Hugo E. Tobar, Francisco J. Salazar-Echegarai, Rodrigo J. Lizana, Carolina P. Quezada, Carlos A. Santiviago, Daniela V. Araya, Claudia A. Riedel, Alexis M. Kalergis, Susan M. Bueno

Resultado de la investigación: Article

19 Citas (Scopus)

Resumen

The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. The whole genome sequence analysis for S. Enteritidis unveiled the presence of several genetic regions that are absent in other Salmonella serovars. These regions have been denominated "regions of difference" (ROD). In this study we show that ROD21, one of such regions, behaves as an unstable pathogenicity island. We observed that ROD21 undergoes spontaneous excision by two independent recombination events, either under laboratory growth conditions or during infection of murine cells. Importantly, we also found that one type of excision occurred at higher rates when S. Enteritidis was residing inside murine phagocytic cells. These data suggest that ROD21 is an unstable pathogenicity island, whose frequency of excision depends on the environmental conditions found inside phagocytic cells.

Idioma originalEnglish
Número de artículoe26031
PublicaciónPLoS ONE
Volumen6
N.º10
DOI
EstadoPublished - 2011

Huella dactilar

pathogenicity islands
Genomic Islands
Salmonella enteritidis
Salmonella
phagocytes
Salmonella Enteritidis
Phagocytes
serotypes
Cells
Bacteria
Genes
Infection
infection
mice
Genome
Prophages
Salmonella enterica
genome
bacteria
gastroenteritis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Citar esto

Quiroz, T. S., Nieto, P. A., Tobar, H. E., Salazar-Echegarai, F. J., Lizana, R. J., Quezada, C. P., ... Bueno, S. M. (2011). Excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells. PLoS ONE, 6(10), [e26031]. https://doi.org/10.1371/journal.pone.0026031
Quiroz, Tania S. ; Nieto, Pamela A. ; Tobar, Hugo E. ; Salazar-Echegarai, Francisco J. ; Lizana, Rodrigo J. ; Quezada, Carolina P. ; Santiviago, Carlos A. ; Araya, Daniela V. ; Riedel, Claudia A. ; Kalergis, Alexis M. ; Bueno, Susan M. / Excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells. En: PLoS ONE. 2011 ; Vol. 6, N.º 10.
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abstract = "The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. The whole genome sequence analysis for S. Enteritidis unveiled the presence of several genetic regions that are absent in other Salmonella serovars. These regions have been denominated {"}regions of difference{"} (ROD). In this study we show that ROD21, one of such regions, behaves as an unstable pathogenicity island. We observed that ROD21 undergoes spontaneous excision by two independent recombination events, either under laboratory growth conditions or during infection of murine cells. Importantly, we also found that one type of excision occurred at higher rates when S. Enteritidis was residing inside murine phagocytic cells. These data suggest that ROD21 is an unstable pathogenicity island, whose frequency of excision depends on the environmental conditions found inside phagocytic cells.",
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Quiroz, TS, Nieto, PA, Tobar, HE, Salazar-Echegarai, FJ, Lizana, RJ, Quezada, CP, Santiviago, CA, Araya, DV, Riedel, CA, Kalergis, AM & Bueno, SM 2011, 'Excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells', PLoS ONE, vol. 6, n.º 10, e26031. https://doi.org/10.1371/journal.pone.0026031

Excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells. / Quiroz, Tania S.; Nieto, Pamela A.; Tobar, Hugo E.; Salazar-Echegarai, Francisco J.; Lizana, Rodrigo J.; Quezada, Carolina P.; Santiviago, Carlos A.; Araya, Daniela V.; Riedel, Claudia A.; Kalergis, Alexis M.; Bueno, Susan M.

En: PLoS ONE, Vol. 6, N.º 10, e26031, 2011.

Resultado de la investigación: Article

TY - JOUR

T1 - Excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells

AU - Quiroz, Tania S.

AU - Nieto, Pamela A.

AU - Tobar, Hugo E.

AU - Salazar-Echegarai, Francisco J.

AU - Lizana, Rodrigo J.

AU - Quezada, Carolina P.

AU - Santiviago, Carlos A.

AU - Araya, Daniela V.

AU - Riedel, Claudia A.

AU - Kalergis, Alexis M.

AU - Bueno, Susan M.

PY - 2011

Y1 - 2011

N2 - The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. The whole genome sequence analysis for S. Enteritidis unveiled the presence of several genetic regions that are absent in other Salmonella serovars. These regions have been denominated "regions of difference" (ROD). In this study we show that ROD21, one of such regions, behaves as an unstable pathogenicity island. We observed that ROD21 undergoes spontaneous excision by two independent recombination events, either under laboratory growth conditions or during infection of murine cells. Importantly, we also found that one type of excision occurred at higher rates when S. Enteritidis was residing inside murine phagocytic cells. These data suggest that ROD21 is an unstable pathogenicity island, whose frequency of excision depends on the environmental conditions found inside phagocytic cells.

AB - The availability of the complete genome sequence of several Salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. This is the case of Salmonella enterica serovar Enteritidis (S. Enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. The whole genome sequence analysis for S. Enteritidis unveiled the presence of several genetic regions that are absent in other Salmonella serovars. These regions have been denominated "regions of difference" (ROD). In this study we show that ROD21, one of such regions, behaves as an unstable pathogenicity island. We observed that ROD21 undergoes spontaneous excision by two independent recombination events, either under laboratory growth conditions or during infection of murine cells. Importantly, we also found that one type of excision occurred at higher rates when S. Enteritidis was residing inside murine phagocytic cells. These data suggest that ROD21 is an unstable pathogenicity island, whose frequency of excision depends on the environmental conditions found inside phagocytic cells.

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