Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin

F. Germán Venegas, Cristian Donoso, Camilo Arriaza, Omar Espinoza-Navarro, María Eugenia Castro, Cristian Torres, Felipe Lillo, Rodríguez B. Héctor

Resultado de la investigación: Article

Resumen

Cypermethrin is a Type II pyrethroid widely used for pests control in agriculture. The principal effect of this insecticide is to alter voltage-dependent sodium channels in the nervous system, but it also affects GABA levels in the CNS of mammals. The cerebellar circuit has as its principal neurotransmitter GABA for transportation of CI-, allowing its inhibitory and modulatory function. In this work, the effects of cypermethrin on the cerebellar cortex are studied. Thirty three adult CF-1 male mice were distributed in three groups: 1) untreated, 2) vehicle (oil) and 3) experimental (cypermethrin in oil at 1/5 DL50). The animals were euthanized at days 1, 8, 17, 25 and 34; then processed for histology (haematoxylin-eosine) and im munohis-tochemistry (Apaf-1 and Ki-67). The Purkinje cells and the im-munoreactive Purkinje and Granular cells were quantified. The results showed a significant decrease in Purkinje cells numbers at all the times studied; also, there was an increase in the expression of Apaf-1 and Ki-67 in the Purkinje and Granular cells in acute and chronic stages. This shows that cypermethrin at 1/5 DL50 produces damage to the cerebellar cortex and induces apoptosis in the Purkinje and Granular cells.

Idioma originalEnglish
Páginas (desde-hasta)816-820
Número de páginas5
PublicaciónInterciencia
Volumen39
N.º11
EstadoPublished - 1 ene 2014

Huella dactilar

cypermethrin
cortex
mice
dosage
gamma-aminobutyric acid
cells
oils
sodium channels
pyrethrins
neurotransmitters
pest control
nervous system
histology
insecticides
apoptosis
mammals
agriculture
animals

ASJC Scopus subject areas

  • General

Citar esto

Germán Venegas, F., Donoso, C., Arriaza, C., Espinoza-Navarro, O., Castro, M. E., Torres, C., ... Héctor, R. B. (2014). Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin. Interciencia, 39(11), 816-820.
Germán Venegas, F. ; Donoso, Cristian ; Arriaza, Camilo ; Espinoza-Navarro, Omar ; Castro, María Eugenia ; Torres, Cristian ; Lillo, Felipe ; Héctor, Rodríguez B. / Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin. En: Interciencia. 2014 ; Vol. 39, N.º 11. pp. 816-820.
@article{e3e0d52219e74d64a6b1682ab0fb00ce,
title = "Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin",
abstract = "Cypermethrin is a Type II pyrethroid widely used for pests control in agriculture. The principal effect of this insecticide is to alter voltage-dependent sodium channels in the nervous system, but it also affects GABA levels in the CNS of mammals. The cerebellar circuit has as its principal neurotransmitter GABA for transportation of CI-, allowing its inhibitory and modulatory function. In this work, the effects of cypermethrin on the cerebellar cortex are studied. Thirty three adult CF-1 male mice were distributed in three groups: 1) untreated, 2) vehicle (oil) and 3) experimental (cypermethrin in oil at 1/5 DL50). The animals were euthanized at days 1, 8, 17, 25 and 34; then processed for histology (haematoxylin-eosine) and im munohis-tochemistry (Apaf-1 and Ki-67). The Purkinje cells and the im-munoreactive Purkinje and Granular cells were quantified. The results showed a significant decrease in Purkinje cells numbers at all the times studied; also, there was an increase in the expression of Apaf-1 and Ki-67 in the Purkinje and Granular cells in acute and chronic stages. This shows that cypermethrin at 1/5 DL50 produces damage to the cerebellar cortex and induces apoptosis in the Purkinje and Granular cells.",
keywords = "Apoptosis, Cell proliferation, Cerebellar cortex, Cerebellum, Cypermethriu, Granular cell, Mouse, Purkinje cells, Toxicology",
author = "{Germ{\'a}n Venegas}, F. and Cristian Donoso and Camilo Arriaza and Omar Espinoza-Navarro and Castro, {Mar{\'i}a Eugenia} and Cristian Torres and Felipe Lillo and H{\'e}ctor, {Rodr{\'i}guez B.}",
year = "2014",
month = "1",
day = "1",
language = "English",
volume = "39",
pages = "816--820",
journal = "Interciencia",
issn = "0378-1844",
publisher = "Interciencia Association",
number = "11",

}

Germán Venegas, F, Donoso, C, Arriaza, C, Espinoza-Navarro, O, Castro, ME, Torres, C, Lillo, F & Héctor, RB 2014, 'Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin', Interciencia, vol. 39, n.º 11, pp. 816-820.

Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin. / Germán Venegas, F.; Donoso, Cristian; Arriaza, Camilo; Espinoza-Navarro, Omar; Castro, María Eugenia; Torres, Cristian; Lillo, Felipe; Héctor, Rodríguez B.

En: Interciencia, Vol. 39, N.º 11, 01.01.2014, p. 816-820.

Resultado de la investigación: Article

TY - JOUR

T1 - Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin

AU - Germán Venegas, F.

AU - Donoso, Cristian

AU - Arriaza, Camilo

AU - Espinoza-Navarro, Omar

AU - Castro, María Eugenia

AU - Torres, Cristian

AU - Lillo, Felipe

AU - Héctor, Rodríguez B.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Cypermethrin is a Type II pyrethroid widely used for pests control in agriculture. The principal effect of this insecticide is to alter voltage-dependent sodium channels in the nervous system, but it also affects GABA levels in the CNS of mammals. The cerebellar circuit has as its principal neurotransmitter GABA for transportation of CI-, allowing its inhibitory and modulatory function. In this work, the effects of cypermethrin on the cerebellar cortex are studied. Thirty three adult CF-1 male mice were distributed in three groups: 1) untreated, 2) vehicle (oil) and 3) experimental (cypermethrin in oil at 1/5 DL50). The animals were euthanized at days 1, 8, 17, 25 and 34; then processed for histology (haematoxylin-eosine) and im munohis-tochemistry (Apaf-1 and Ki-67). The Purkinje cells and the im-munoreactive Purkinje and Granular cells were quantified. The results showed a significant decrease in Purkinje cells numbers at all the times studied; also, there was an increase in the expression of Apaf-1 and Ki-67 in the Purkinje and Granular cells in acute and chronic stages. This shows that cypermethrin at 1/5 DL50 produces damage to the cerebellar cortex and induces apoptosis in the Purkinje and Granular cells.

AB - Cypermethrin is a Type II pyrethroid widely used for pests control in agriculture. The principal effect of this insecticide is to alter voltage-dependent sodium channels in the nervous system, but it also affects GABA levels in the CNS of mammals. The cerebellar circuit has as its principal neurotransmitter GABA for transportation of CI-, allowing its inhibitory and modulatory function. In this work, the effects of cypermethrin on the cerebellar cortex are studied. Thirty three adult CF-1 male mice were distributed in three groups: 1) untreated, 2) vehicle (oil) and 3) experimental (cypermethrin in oil at 1/5 DL50). The animals were euthanized at days 1, 8, 17, 25 and 34; then processed for histology (haematoxylin-eosine) and im munohis-tochemistry (Apaf-1 and Ki-67). The Purkinje cells and the im-munoreactive Purkinje and Granular cells were quantified. The results showed a significant decrease in Purkinje cells numbers at all the times studied; also, there was an increase in the expression of Apaf-1 and Ki-67 in the Purkinje and Granular cells in acute and chronic stages. This shows that cypermethrin at 1/5 DL50 produces damage to the cerebellar cortex and induces apoptosis in the Purkinje and Granular cells.

KW - Apoptosis

KW - Cell proliferation

KW - Cerebellar cortex

KW - Cerebellum

KW - Cypermethriu

KW - Granular cell

KW - Mouse

KW - Purkinje cells

KW - Toxicology

UR - http://www.scopus.com/inward/record.url?scp=84911465925&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84911465925

VL - 39

SP - 816

EP - 820

JO - Interciencia

JF - Interciencia

SN - 0378-1844

IS - 11

ER -

Germán Venegas F, Donoso C, Arriaza C, Espinoza-Navarro O, Castro ME, Torres C y otros. Evaluation of the effects on the cerebellar cortex of CF-1 mice exposed to a single dose of cypermethrin. Interciencia. 2014 ene 1;39(11):816-820.